Myelin transcription factor 1 (Myt1) modulates the proliferation and differentiation of oligodendrocyte lineage cells

Nielsen, Joseph A.; Berndt, Jo Ann; Hudson, Lynn D.; Armstrong, Regina C.

doi:10.1016/j.mcn.2003.10.001

Cited by 97 publications

(120 citation statements)

References 35 publications

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“…Retrovirus generation and infections were performed as previously described [16,25]. Dr. Kitamura (Tokyo University) provided the PMXs replication-incompetent retroviral vector containing a multiple cloning site followed by an internal ribosome entry site (IRES) sequence and enhanced GFP [10].…”

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

“…OP cultures were incubated with retrovirus for 6 hrs (approximately 3000 CFU/well which typically resulted in GFP expression among the majority of cells). The cells were maintained for 2 days in defined medium with PDGF and FGF2, and refed medium to promote differentiation (see above) over 3 days prior to fixation and immunostaining [16,25].…”

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

“…Cultures were immunostained for O1 or glial fibrillary acidic protein (GFAP) to monitor differentiation of infected OP cells into oligodendrocytes or astrocytes, respectively [16]. To estimate cell proliferation, bromodeoxyuridine (BrdU; 2 μM; Sigma) was added for 20 hrs of culture prior to fixing and immunostaining cells to detect BrdU incorporation [16,25].…”

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

“…To estimate cell proliferation, bromodeoxyuridine (BrdU; 2 μM; Sigma) was added for 20 hrs of culture prior to fixing and immunostaining cells to detect BrdU incorporation [16,25]. The Chisquare test with the Mantel-Haenzel subgroup analysis was used to compare immunostaining results among retrovirally labeled cell populations.…”

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

“…OP cells were co-transfected with pHes1 or pHes5 along with pRL-CMV (SV40 promoter drives expression of Renilla luciferase; Promega) to estimate transfection efficiency [16]. After incubation overnight (16 hrs), the cells were then transferred to defined medium with or without FGF2 (10 ng/ml) and grown for 10 hrs.…”

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

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Interaction of fibroblast growth factor 2 (FGF2) and notch signaling components in inhibition of oligodendrocyte progenitor (OP) differentiation

Zhou

Armstrong

2007

Neuroscience Letters

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Oligodendrocyte progenitor (OP) cell differentiation is a critical process of developmental myelination, tumor formation, and remyelination in the CNS. Activation of the fibroblast growth factor 2 (FGF2) or notch pathway can inhibit differentiation of OP cells. The current study examines the interaction of FGF2 and notch signaling components in regulating OP differentiation. Cultured neonatal rat brain OP cells were used for transfection-based promoter assays and for infection with retroviruses expressing a GFP reporter to monitor OP differentiation into oligodendrocytes or astrocytes. FGF2 treatment resulted in a 4-fold increase of transcriptional activity from the promoter region of Hes5, a notch pathway target gene. FGF2 inhibition of OP differentiation into oligodendrocytes was perturbed by retroviral expression of a dominant negative construct for mastermind-like 1, which is an important co-activator of transcription for notch target genes. OP differentiation into oligodendrocytes was reduced by co-culture with fibroblasts expressing Jagged1, a ligand for notch receptors. This Jagged1 inhibition of OP differentiation was not altered by retroviral expression of a dominant negative FGF receptor construct. Constitutive activation of notch signaling, by retroviral expression of the Notch1 intracellular domain, greatly reduced OP differentiation into either oligodendrocytes or astrocytes and did not require FGF2 signaling. These findings indicate that inhibition of OP differentiation through the Notch1 pathway was not influenced by FGF2 signaling. However, FGF2 signaling may interact with down stream components of the notch signaling pathway, including mastermind-like1 and Hes5, to inhibit OP differentiation into oligodendrocytes.

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Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

Section: Op Cultures Retroviral Vectors Infections and Immunocytocmentioning

confidence: 99%

See 3 more Smart Citations

Interaction of fibroblast growth factor 2 (FGF2) and notch signaling components in inhibition of oligodendrocyte progenitor (OP) differentiation

Zhou

Armstrong

2007

Neuroscience Letters

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Genome‐wide supported psychosis risk variant in ZNF804A gene and impact on cortico–limbic WM integrity in schizophrenia

Kuswanto

Woon

Zheng

et al. 2012

American J of Med Genetics Pt B

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Genome-wide association, case association genetic and meta-analytic studies have highlighted ZNF804A as a robust genome-wide supported susceptibility gene for schizophrenia (SCZ). In view of the possible involvement of ZNF804A gene in early neurodevelopment and cellular processes including oligodendrocyte proliferation and differentiation, we examined the effect of ZNF804A on brain WM (WM) integrity in patients with SCZ. Based on extant data in healthy controls (HC), we hypothesized that ZNF804A risk variant rs1344706 is associated with lower fractional anisotropy (FA) in brain regions within cortico-limbic circuits, namely frontal, parietal, medial temporal lobes, and cingulate gyri in SCZ. A total of 200 Chinese participants (125 patients with DSM-IV diagnosis of SCZ and 75 controls) were genotyped using blood samples, a subset of 153 participants (89 patients with DSM-IV diagnosis of SCZ and 64 controls) underwent structural magnetic resonance imaging and diffusion tensor imaging (DTI). There are significant effects of diagnosis (left cingulate gyrus: Adjusted F(1,149) = 9.36, P = 0.003) and diagnosis-genotype interactions (left parietal lobe: Adjusted F(1,147) = 7.39, P = 0.007; right parietal lobe: Adjusted F(1,147) = 6.95, P = 0.009; right medial temporal lobe: Adjusted F(1,147) = 8.79, P = 0.004; left cingulate gyrus: Adjusted F(1,147) = 8.02, P = 0.005). Specifically, we found that patients with SCZ who are risk T homozygotes have lower FA in bilateral parietal lobes, and left cingulate gyrus compared with G carriers. Compared with risk T homozygotes in HC, patients with SCZ who are risk T homozygotes have decreased FA in bilateral parietal lobes, and left cingulate gyrus as well as right medial temporal lobe. Our findings suggest that ZNF804A risk variant influence WM integrity involving cortico-limbic brain regions in SCZ and highlight the importance of investigating the impact of genome-wide supported risk factors on intermediate phenotypes with potential to shed light on the neurobiology of SCZ.

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Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development

Matsushita

Kameyama

Kadokawa

et al. 2013

Developmental Dynamics

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Background: Three members of the Myt/NZF family of transcription factors are involved in many processes of vertebrate development. Several studies have reported that Myt1/NZF-2 has a regulatory function in the development of cultured oligodendrocyte progenitors or in neuronal differentiation during Xenopus primary neurogenesis. However, little is known about the proper function of Myt/NZF family proteins during mammalian nervous system development. To assess the possible function of Myt/NZF transcription factors in mammalian neuronal differentiation, we determined the comparative spatial and temporal expression patterns of all three types of Myt/NZF family genes in the embryonic mouse nervous system using quantitative reverse transcriptase polymerase chain reaction and in situ hybridization. Results: All three Myt/NZF family genes were extensively expressed in developing mouse nervous tissues, and their expression was transient. NZF-1 was expressed later in post-mitotic neurons. NZF-2 was initially expressed in neuronal cells a little earlier than NZF-3. NZF-3 was initially expressed in neuronal cells, just after proliferation was complete. Conclusion: These expression patterns suggest that the expression of NZF family genes is spatially and temporally regulated, and each Myt/NZF family gene may have a regulatory function in a specific phase during neuronal differentiation. Developmental Dynamics 243:588-600, 2014. Developmental DynamicsABBREVIATIONS APa archipallium BAM Brn2, Ascl1, and Myt1l BAMN Brn2, Ascl1, Myt1l, and NeuroD1 BG basal ganglia BrdU bromodeoxyuridine CA1, CA3 cornu ammonis fields CBL cerebellum CNS central nervous system CP cortical plate cSp cervical spinal cord CTX cortex DG dentate gyrus DIG digoxigenin dpc days post coitum DRG dorsal root ganglion DTe dorsal telencephalon EGL external germinal layer of the cerebellum fIC fibers of the internal capsule HC hippocampus HT hypothalamus IC internal capsule iCo inferior colliculus iNBL inner neuroblastic layer of the neural retina Is isthmus ISH in situ hybridization IXg glossopharyngeal ganglia IZ intermediate zone LDN lateral deep nucleus LGE lateral ganglionic eminence Me medulla MGE medial ganglionic eminence ML mantle layer MN motor neuron Myt myelin transcription factor NZF neural zinc finger oNBL outer neuroblastic layer of the neural retina OpV optic vesicle OtV otic vesicle Pal pallidum PBS phosphate-buffered saline POA preoptic area PP preplate Ps pons sCo superior colliculus SVZ subventricular zone Str striatum sXg superior ganglion of the vagus nerve Teg tegmentum TH thalamus UTR untranslated region Vg trigeminal ganglion VIIg facial ganglion VIIIg vestibulocochlear ganglion VII-VIII facio-acoustic neural crest complex VTe ventral telencephalon VZ ventricular zone WM white matter Xg vagus ganglia.

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Myelin transcription factor 1 (Myt1) modulates the proliferation and differentiation of oligodendrocyte lineage cells

Cited by 97 publications

References 35 publications

Interaction of fibroblast growth factor 2 (FGF2) and notch signaling components in inhibition of oligodendrocyte progenitor (OP) differentiation

Interaction of fibroblast growth factor 2 (FGF2) and notch signaling components in inhibition of oligodendrocyte progenitor (OP) differentiation

Genome‐wide supported psychosis risk variant in ZNF804A gene and impact on cortico–limbic WM integrity in schizophrenia

Spatiotemporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development

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