Myeloperoxidase in blood neutrophils during normal and abnormal menstrual cycles in women of reproductive age

Shibata, Takeo; Sakamoto, Jinichi; Osaka, Yasuhiro; Neyatani, N.; Fujita, Satoshi; Oka, Y.; Takagi, Hiroaki; H, Mori; Fujita, Haruyuki; Tanaka, Yuichiro; Sasagawa, Toshiyuki

doi:10.1111/ijlh.12599

Cited by 3 publications

(3 citation statements)

References 24 publications

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“… 25 , 26 Cluster 4 was annotated as neutrophil (NP) with high expression of CTSG, ELANE, PRTN3, AZU1, and MPO. 27 , 28 , 29 Cluster 6 was enriched expression of TLR2, ITGAM, CD163, and PECAM1, which was annotated as monocyte. 30 , 31 Cluster 8 was annotated as dendritic cell (DC) with high expression of CD1C, FCER1A, SIRPA, and ITGAX.…”

Section: Resultsmentioning

confidence: 99%

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Yang

Shi

et al. 2021

JOR Spine

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Backgrounds: Abnormal vertebral growth and development have been found in adolescent idiopathic scoliosis (AIS) patients, and the proliferation and differentiation of bone development-related cells play important roles in its pathogenesis. However, a comprehensive single-cell-level differentiation roadmap in AIS has not been achieved. Methods:The present study compared the single-cell level cellular landscapes of spinal cancellous bone tissues between AIS patients and healthy subjects using high throughput single-cell RNA sequencing (scRNA-seq), which covers multiple cellular lineages including osteoblast, chondrocyte, osteoclast and related immunocytes. We constructed the differentiation trajectories of bone development-related cell lineages through pseudotime analysis, and the intercellular-communication networks between bone development-related cells and immunocytes were further developed.Results: A total of 11 distinct cell clusters were identified according to the genome-wide transcriptome profiles. t-Distributed stochastic neighbor embedding (t-SNE) analysis showed that mesenchymal stem cells (MSC) were classified into three subtypes: MSC-LOXL2, MSC-IGFBP5, and MSC-GJA1. Gene ontology (GO) analysis showed that MSC-GJA1 might possess greater osteoblast differentiation potential than the others. MSC-IGFBP5 was the specific MSC subtype observed only in AIS. There were two distinct gene expression clusters: OB-DPT and OB-OLFML2B, and the counts of osteoblasts derived from AIS was significantly less than that of non-AIS subjects. In AIS patients, MSC-IGFBP5 failed to differentiate into osteoblasts and exhibited negative regulation of cell proliferation and enhanced cell death. CPC-PCNA was found to be the specific chondrocyte progenitor cell (CPC) subtype observed only in AIS patients. The cell counts of OC-BIRC3 in AIS were less than those in controls. Pseudotime analysis suggested two possible distinct osteoclast differentiation patterns in AIS and control subjects. Monocytes in AIS mainly differentiated into OC-CRISP3.

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Section: Resultsmentioning

confidence: 99%

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Yang

Shi

et al. 2021

JOR Spine

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“…The cyclical expression of MPO, as well as the total absence of glandular staining in endometrial cells in anestrus, is concordant with a protein expression regulated by steroid hormones. The activity of MPO in blood neutrophils seems to be regulated by the menstrual cycle in women [54,55] and an upregulation of MPO activity by estrogens has been suggested, probably through the direct stimulation of the expression of the MPO gene [55]. In uterine tissues of rats, MPO activity was reported to increase during the follicular phase of the cycle, decrease thereafter during the luteal phase, and reach minimal values in late diestrus samples [56].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Myeloperoxidase in the Healthy Equine Endometrium

Hernandez

Franck

Munaut

et al. 2023

Animals

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Myeloperoxidase (MPO), as a marker of neutrophil activation, has been associated with equine endometritis. However, in absence of inflammation, MPO is constantly detected in the uterine lumen of estrous mares. The aim of this study was to characterize MPO in the uterus of mares under physiological conditions as a first step to better understand the role of this enzyme in equine reproduction. Total and active MPO concentrations were determined, by ELISA and SIEFED assay, respectively, in low-volume lavages from mares in estrus (n = 26), diestrus (n = 18) and anestrus (n = 8) in absence of endometritis. Immunohistochemical analysis was performed on 21 endometrial biopsies randomly selected: estrus (n = 11), diestrus (n = 6) and anestrus (n = 4). MPO, although mostly enzymatically inactive, was present in highly variable concentrations in uterine lavages in all studied phases, with elevated concentrations in estrus and anestrus, while in diestrus, concentrations were much lower. Intracytoplasmic immunoexpression of MPO was detected in the endometrial epithelial cells, neutrophils and glandular secretions. Maximal expression was observed during estrus in mid and basal glands with a predominant intracytoplasmic apical reinforcement. In diestrus, immunopositive glands were sporadic. In anestrus, only the luminal epithelium showed residual MPO immunostaining. These results confirm a constant presence of MPO in the uterine lumen of mares in absence of inflammation, probably as part of the uterine mucosal immune system, and suggest that endometrial cells are a source of uterine MPO under physiological cyclic conditions.

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“…Consortium via the PRIDE partner repository 41 To verify proteomic and RNA-seq data quality, we tested expression of surface proteins used to isolate the various HSPC subpopulations as well as lineage markers with known expression in myeloid progenitors. CD34, CD38, the granulocytic marker MPO 42 and the erythroid precursor marker TFRC, a.k.a. CD71, 43 were detected at expected intensities in specific HSPC subpopulations on protein and RNA level (supplementary figure S3).…”

Section: Data Sharing Statement the Proteomics Data Have Been Deposited To The Proteomexchangementioning

confidence: 99%

Proteomic identification of proliferation and progression markers in human polycythemia vera stem and progenitor cells

et al. 2022

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Polycythemia vera (PV) is a stem cell disorder characterized by hyperproliferation of the myeloid lineages and the presence of an activating JAK2 mutation. To elucidate mechanisms controlling PV stem and progenitor cell biology, we applied a recently developed highly sensitive data-independent acquisition mass spectrometry workflow to purified hematopoietic stem and progenitor cell (HSPC) subpopulations of patients with chronic and progressed PV. We integrated proteomic data with genomic, transcriptomic, flow cytometry and in vitro colony formation data. Comparative analyses revealed added information gained by proteomic compared with transcriptomic data in 30% of proteins with changed expression in PV patients. Upregulated biological pathways in hematopoietic stem and multipotent progenitor cells (HSC/MPPs) of PV included MTOR, STAT and interferon signaling. We further identified a prominent reduction of clusterin (CLU) protein expression and a corresponding activation of NFĸB signaling in HSC/MPPs of untreated PV patients compared with controls. Reversing the reduction of CLU and inhibiting NFĸB signaling decreased proliferation and differentiation of PV HSC/MPPs in vitro. Upon progression of PV, we identified upregulation of LGALS9 and SOCS2 protein expression in HSC/MPPs. Treatment of patients with hydroxyurea normalized the expression of CLU and NFĸB2, but not of LGALS9 and SOCS2. These findings expand the current understanding of the molecular pathophysiology underlying PV and provide new potential targets (CLU and NFĸB) for antiproliferative therapy in PV patients.

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Myeloperoxidase in blood neutrophils during normal and abnormal menstrual cycles in women of reproductive age

Abstract: The difference in MPO between women with abnormal and normal menstrual cycles and the upregulation of MPO before ovulation suggest that neutrophils and MPO are closely related to ovulation.

Cited by 3 publications

References 24 publications

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Characterization of Myeloperoxidase in the Healthy Equine Endometrium

Proteomic identification of proliferation and progression markers in human polycythemia vera stem and progenitor cells

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