Myeloperoxidase of bronchial leucocytes as a biomarker of osmotic bronchial hyperresponsiveness in asthmatics

Perelman, Juliy M.; Пирогов, А. Б.; Prikhodko, Anna; Zinoviev, S.V.; Кolosov, Victor P.; Мальцева, Т. А.

doi:10.1183/13993003.congress-2015.pa3872

Cited by 2 publications

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“…[10] In our previous studies we found the elevated level of MPO activity is associated with high intensiveness of degranulation and destruction of leukocytes in IS of asthma patients with AHR to hypoosmotic and cold triggers. [13,14] In light of a well-established connection between oxidative stress and inflammation in asthma, the relationship between airway inflammation and LP in the formation of excessive airway response to osmotic stimulus deserves special attention. The aim of our study was to evaluate the role of airway cellular inflammation and the LP level in the development of AHR to IHS.…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of Airway Inflammation and Lipid Peroxidation in the Development of Hyperosmotic Airway Hyperresponsiveness in Patients with Asthma

Pirogov¹,

Prikhodko²,

Perelman³

et al. 2016

Int J Biomed

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The aim of our study was to evaluate the role of airway cellular inflammation and the lipid peroxidation level in the development of airway hyperresponsiveness (AHR) to inhalation of hypertonic saline (IHS). Methods and Results:The study included the estimation of inflammatory-cellular composition, intracellular concentration of myeloperoxidase (MPO) in induced sputum (IS), serum levels of lipid hydroperoxides (LHP), ceruloplasmin, and vitamin E in 29 patients with asthma and 12 healthy persons. AHR to IHS was assessed by spirometry after 3-min IHS via ultrasonic nebulizer. Patients with asthma had higher indices of leukocytes destruction and cytolysis intensity with the increased leukocyte count in IS. Maximum values of neutrophils cytolysis intensity and leukocytic MPO were found in IS of the patients with AHR to IHS. After the bronchial provocation, serum concentration of LHP was higher in these patients in comparison with the patients without the AHR and control groups. In addition, patients with asthma had lower level of antioxidants than healthy subjects.Conclusion: Marked inflammation involving MPO-activated leukocytes and intensive lipid peroxidation underlie the excessive airway response to IHS. (Int J Biomed. 2016; 6(4):259-264.)

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Section: Introductionmentioning

confidence: 99%

Peculiarities of Airway Inflammation and Lipid Peroxidation in the Development of Hyperosmotic Airway Hyperresponsiveness in Patients with Asthma

Pirogov¹,

Prikhodko²,

Perelman³

et al. 2016

Int J Biomed

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Influence of Neutrophilic Component of Bronchial Inflammation on the Level of Disease Control and Lung Function in Patients With Asthma

Приходько¹,

Prikhodko²,

Пирогов³

et al. 2016

Bulletin Physiology and Pathology of Respiration

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The cell pattern of bronchi inflammatory infiltrate has a significant impact on the course of the disease and may affect the achievement and maintenance of the asthma control. The aim of the study was to determine the influence of neutrophilic component of the bronchial inflammation on the asthma control, lung function and airway responsiveness. 114 patients with mild persistent asthma were assessed upon the level of the asthma control by Asthma Control Test (ACT); lung function (FEV1) by spirometry; airway responsiveness (∆FEV1) to 3-minute isocapnic hyperventilation with cold air (-20ºС) (IHCA) and 3-minute ultrasound inhalation with distilled water (IDW). The cell composition of the induced sputum (IS) was also studied. The patients were divided into groups with low contents of neutrophils in IS (39 people; 1st group) and with high contents of neutrophils (75 people, 2nd group). The contents of neutrophils in the 1st group was 10.9±0.53%, eosinophils – 19.4±2.1%; in the 2nd group – 29.9±1.6% (р=0.0001) and 20.4±1.4% (р=0.66), respectively. By ACT data the people of the 2nd group managed their disease worse than in 1st group (15.0±0.6 and 19.0±0.8 points, respectively; р=0.0001). They also had lower FEV1 (88.0±2.0 and 96.7±2.4%; р=0.009) and more intensive airway response to IHCA and DW (-13.7±1.7% and -11.2±1.9%, respectively) in comparison with the 1st group (-2.7±0.86%; р=0.0001 and -5.3±1.90%; р=0.055). The patients of the 2nd group had a close correlation between baseline neutrophils in IS and ∆FEV1 in response to IHCA (r=-0.22; р=0.045) as well as the content of myeloperoxidase in IS (r=0.31; р=0.0008). A level of myeloperoxidase in IS correlated with ∆FEV1 in response to IDW (r=0.29; р=0.030) in these patients. The increase of neutrophilic component of inflammation in asthma patients worsens the asthma control, lung function and enhances airway responsiveness to exogenous stimuli.

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Myeloperoxidase of bronchial leucocytes as a biomarker of osmotic bronchial hyperresponsiveness in asthmatics

Cited by 2 publications

References 0 publications

Peculiarities of Airway Inflammation and Lipid Peroxidation in the Development of Hyperosmotic Airway Hyperresponsiveness in Patients with Asthma

Peculiarities of Airway Inflammation and Lipid Peroxidation in the Development of Hyperosmotic Airway Hyperresponsiveness in Patients with Asthma

Influence of Neutrophilic Component of Bronchial Inflammation on the Level of Disease Control and Lung Function in Patients With Asthma

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