Myeloperoxidase, xanthine oxidase and superoxide dismutase in the gastric mucosa of helicobacter pylori positive and negative pediatric patients

Abstract

The aim of this study was determination and comparison of the levels of myeloperoxidase (MPO), xanthine oxidase (XO), and superoxide dismutase (SOD) in gastric mucosa of children who were infected and noninfected with Helicobacter pylori (HP). The MPO, and XO enzyme activities were detected via kinetic measurement, and the MPO, XO and SOD enzyme protein levels were detected via Western blot, in antral mucosa specimens of 43 patients who underwent upper gastrointestinal endoscopy with various indications. The diagnosis of HP infection was made with a positive rapid urease test and histopathologic detection. MPO activity and enzyme protein levels were measured in 14 [8 HP (+) and 6 HP (-)], and in 9 [5 HP (+) and 4 HP (-)] while XO activity and enzyme protein levels were measured in 16 [10 HP (+) and 6 HP (-)] and in 9 [5 HP (+) and 4 HP (-)] patients, respectively. SOD protein level was detected in 13 [7 HP (+) and 6 HP (-)] patients. Of 43 patients 25 were HP (+) and 18 were HP (-). MPO activities were 75.6 +/- 40.5 and 98.8 +/- 44.1 U/g. protein (p = 0.302) while XO activities were 0.5 +/- 0.3 and 0.4 +/- 0.2 U/g. protein in HP (+) and HP (-) patients, respectively (p = 0.625). Measured enzyme protein levels of MPO, XO and SOD were found statistically indifferent in HP (+) and HP (-) patients (p = 0.327, p = 0.086, and p = 0.775, respectively). The results of this study revealed that, MPO, XO and SOD conditions in gastric mucosa alone were not affected from HP presence. That's why MPO, XO, and SOD may not have important roles in the pathogenesis of HP related gastric disease in children.

A Study of Oxidative Stress Parameters in Anti-Helicobacter Pylorus Immunoglobulin G Positive and Negative Gastric Cancer Patients

“…Some previous studies have reported that no CAT activity was found in most gastric juices, probably because of their low pH levels, and in some antral tissue specimens 41,42. Akcan, et al2 reported no unchanged of MPO and SOD activity in gastric mucosa of childhood period with HP (+) and (-) subjects, and Bulbuloglu, et al42 also reported no unchanged of CAT activity in antral mucosal between HP (+) and (-) subjects. In the present study, MPO and CAT activities were similar between the subjects included in the study.…”

“…Gastric cancer is usually diagnosed after the 5th decade of life, but HP infection usually occurs in childhood and remains active lifelong unless treated. A cohort study has shown a link between early infection and increased cancer risk 2,33. In the present study, the level of AOOP was increased significantly in the gastric cancer patients as compared with non-gastric cancer patients.…”

“…HP infection is a worldwide pathogenic condition in the development of infection, causing different problems in the gastric mucosa 2. In addition, some chronic infections, acquired early in life, confer an increased risk of gastric cancer 3,4.…”

Saccharomyces Boulardii in Helicobacter Pylori Eradication in Children: A Randomized Trial From Iran

“…H. pylori is considered to be the major cause of chronic gastritis and duodenal ulcer in childhood and an important cofactor in the development of gastric cancer or mucosa-associated lymphoid tissue (MALT) lymphoma ( 7 , 8 ). Therefore, H. pylori eradication in childhood will not only result in peptic symptoms relief, but will also prevent late-term complications, such as cancer ( 9 ).…”

The effects of probiotics on treatment of Helicobacter pylori eradication in children

“… 2 , 3 The eradication of H. pylori in childhood is important not only to relieve peptic symptoms, but also to prevent late-term complications, such as cancer. 4 To date, numerous treatment regimens have been attempted to eradicate the organism; however, the success rates are far from ideal. The most widely accepted first line treatment regimen includes the combination of a proton pump inhibitor and 2 antibiotics (amoxicillin and clarithromycin).…”