Background:The non-ocular function(s) of myocilin, a glaucoma-associated protein, is not known. Results: Myocilin interacts with syntrophin, a component of dystrophin-associated protein complex (DAPC) and increases phosphorylation of several regulators of muscle size. Conclusion: Myocilin is involved in the muscle hypertrophy pathways acting through the components of DAPC. Significance: This is the first demonstration of myocilin functions in the skeletal muscles.