MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos

Hopwood, Nick; Pluck, A.; Gurdon, J. B.

doi:10.1016/0168-9525(89)90157-1

Cited by 114 publications

(166 citation statements)

References 23 publications

(44 reference statements)

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“…Experiments using knock-out mice showed that MyoD and Myf-5 play overlapping roles in myoblast specification, whereas myogenin and either MyoD or MRF4 are required for differentiation (Tajbakhsh and Buckingham, 2000). In contrast to what is observed in the murine model where the first expression of Myf-5 and MyoD is somitic, in Xenopus embryos, these two MRFs can both be detected in presomitic mesoderm at the transcript level (Hopwood et al, 1989Scales et al, 1990), and at the protein level for MyoD (Hopwood et al, 1992). XMyf-5 expression is first visible at the early gastrula stage in two symmetrical dorsolateral domains, whereas early gastrula expression of XMyoD occurs in the entire marginal zone with the exception of the dorsal domain (Frank and Harland, 1991;Dosch et al, 1997).…”

Section: Establishment Of Muscle Lineages During Early Embryogenesismentioning

confidence: 83%

“…The first activation of muscle-specific genes is observed during gastrulation and concerns the two myogenic regulatory factors XMyf-5 and XMyoD (Hopwood et al, 1989;Harvey, 1991), whose transcripts are detected at stages 9.5 and 10.5, respectively, quickly followed by the accumulation of transcripts of ␣ cardiac actin (Mohun et al, 1988(Mohun et al, , 1994, the first muscle structural gene expressed in vertebrates (Sassoon, 1993). The myogenic regulatory factors (MRFs) of the MyoD family (including MyoD itself, Myf-5, myogenin, and MRF4) are basic helix-l (bHLH) helix transcription factors whose ectopic expression is able to convert a wide range of cultured cells to a muscle phenotype and which can promote the transcription of several muscle-specific genes.…”

Section: Establishment Of Muscle Lineages During Early Embryogenesismentioning

confidence: 99%

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Xenopusmuscle development: From primary to secondary myogenesis

Chanoine

Hardy

2002

Developmental Dynamics

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Xenopus myogenesis is characterized by specific features, different from those of mammalian and avian systems both at the cellular level and in gene expression patterns. During early embryogenesis, after the initial molecular signals inducing mesoderm, the myogenic determination factors XMyoD and XMyf-5 are activated in presomitic mesoderm in response to mesoderm-inducing factors. After these first inductions of the myogenic program, forming muscles in Xenopus can have different destinies, some of these resulting in cell death before adulthood. In particular, it is quite characteristic of this species that, during metamorphosis, the primary myotomal myofibers completely die and are progressively replaced by secondary "adult" multinucleated myofibers. This feature offers the unique opportunity to totally separate the molecular analysis of these two distinct types of myogenesis. The aim of this review is to summarize our knowledge on the cellular and molecular events as well as the epigenetic regulations involved in the construction of Xenopus muscles during development. Developmental Dynamics 226:12-23, 2003.

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Section: Establishment Of Muscle Lineages During Early Embryogenesismentioning

confidence: 83%

Section: Establishment Of Muscle Lineages During Early Embryogenesismentioning

confidence: 99%

Xenopusmuscle development: From primary to secondary myogenesis

Chanoine

Hardy

2002

Developmental Dynamics

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“…RNA purified from 10 embryos was loaded into each lane. Northern blotting was performed as described (Hopwood et al, 1989). Ethidium bromide staining of ribosomal RNA was used as a loading control.…”

Section: Fig 1 Zebrafish Frizzled-2 Sequence Analysis and Expressiomentioning

confidence: 99%

Zebrafish frizzled‐2 morphant displays defects in body axis elongation

Sumanas

Kim

Hermanson

et al. 2001

Genesis

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“…3E). Besides ␣-actin, expression of other mesodermal markers, such as derriere (Sun et al, 1999a), brachyury (Cunliffe and Smith, 1992), and myoD (Hopwood et al, 1989), was elevated in the Neptune-injected caps (data not shown). Thus, it was shown that Neptune has an activity to induce mesoderm directly from animal cap cells even though Neptune is a DNAbinding protein and a factor related to erythropoietic differentiation (Kawahara and Dawid, 2000;Huber et al, 2001).…”

Section: Mesoderm-inducing Activity Of Neptune In Animal Cap Explantsmentioning

confidence: 93%

Neptune is involved in posterior axis and tail formation in Xenopus embryogenesis

Takeda

Kurauchi

Yamazaki

et al. 2005

Developmental Dynamics

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In order to elucidate the molecular mechanisms underlying the posterior axis and tail formation in embryogenesis, the function of Neptune, a zinc-finger transcription factor, in Xenopus laevis embryos was investigated. Injection of neptune mRNA into the animal pole area of embryos resulted in the formation of an additional tail structure that included a neural tube and muscle tissue. This activity required FGF signaling since coinjection of a dominant-negative FGF receptor RNA (XFD) completely blocked the formation of a tail structure. A loss-of-function experiment using a fusion construct of neptune and Drosophila engrailed (en-neptune) RNA showed that endogenous Neptune is necessary for formation of the posterior trunk and tail. Furthermore, activity of Neptune was necessary for the endogenous expression of brachyury and fgf-8 at the late gastrula stage. These findings demonstrate a novel function of Neptune in the process of anterior-posterior axis formation through the FGF and brachyury signaling cascades. An experiment using a combination explant with ventral and dorsal marginal tissues showed that cooperation of these two distinct tissues is important for the tail formation and that expression of Neptune in prospective ventral cells may be involved in the activation of the process of tail formation. Developmental Dynamics 234:63-73, 2005.

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MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos

Cited by 114 publications

References 23 publications

Xenopusmuscle development: From primary to secondary myogenesis

Xenopusmuscle development: From primary to secondary myogenesis

Zebrafish frizzled‐2 morphant displays defects in body axis elongation

Neptune is involved in posterior axis and tail formation in Xenopus embryogenesis

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