Myogenic stem cells.

Burdzińska, Anna; Gala, Kamila; Pączek, Leszek

doi:10.2478/v10042-008-0060-1

Cited by 13 publications

(9 citation statements)

References 87 publications

(135 reference statements)

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“…Therefore, it can be concluded that isolated population predominantly consisted of myoblasts. However, as heterogeneity of the population isolated from skeletal muscle tissue is well known [25], we’d rather call the obtained population "muscle-derived cells". Bone marrow-derived cells, typically for a MSC population, displayed multilineage differentiation capacity.…”

Section: Discussionmentioning

confidence: 99%

The Mutual Interactions between Mesenchymal Stem Cells and Myoblasts in an Autologous Co-Culture Model

et al. 2016

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Both myoblasts and mesenchymal stem cells (MSC) take part in the muscle tissue regeneration and have been used as experimental cellular therapy in muscular disorders treatment. It is possible that co-transplantation approach could improve the efficacy of this treatment. However, the relations between those two cell types are not clearly defined. The aim of this study was to determine the reciprocal interactions between myoblasts and MSC in vitro in terms of the features important for the muscle regeneration process. Primary caprine muscle-derived cells (MDC) and bone marrow-derived MSC were analysed in autologous settings. We found that MSC contribute to myotubes formation by fusion with MDC when co-cultured directly, but do not acquire myogenic phenotype if exposed to MDC-derived soluble factors only. Experiments with exposure to hydrogen peroxide showed that MSC are significantly more resistant to oxidative stress than MDC, but a direct co-culture with MSC does not diminish the cytotoxic effect of H2O2 on MDC. Cell migration assay demonstrated that MSC possess significantly greater migration ability than MDC which is further enhanced by MDC-derived soluble factors, whereas the opposite effect was not found. MSC-derived soluble factors significantly enhanced the proliferation of MDC, whereas MDC inhibited the division rate of MSC. To conclude, presented results suggest that myogenic precursors and MSC support each other during muscle regeneration and therefore myoblasts-MSC co-transplantation could be an attractive approach in the treatment of muscular disorders.

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Section: Discussionmentioning

confidence: 99%

The Mutual Interactions between Mesenchymal Stem Cells and Myoblasts in an Autologous Co-Culture Model

et al. 2016

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“…Long‐term culturing of human muscle‐derived cells promotes the expression of phenotypic and functional markers typical for mesenchymal stem cells (Lecourt et al, ). According to the expression of stem cell markers, CD34 and Sca‐1, our established cell lines may contain MDSC (Usas and Huard, ; Burdzińska et al, ; Wu et al, ). Moreover, distinct proportions of stem cell subpopulations at different maturation stages may exist at different times and under different conditions in culture.…”

Section: Discussionmentioning

confidence: 99%

Long‐term muscle‐derived cell culture: multipotency and susceptibility to cell death stimuli

Kalvelytė

Krestnikova

Stulpinas

et al. 2013

Cell Biology International

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Improvement in the yield of adult organism stem cells, and the ability to manage their differentiation and survival potential are the major goals in their application in regenerative medicine and in the adult stem cell research. We have demonstrated that adult rabbit muscle-derived cell lines with an unlimited proliferative potential in vitro can differentiate into myogenic, osteogenic, adipogenic and neurogenic lineages. Studies of cell survival in vitro showed that differentiated cells, except neurogenic ones, are more resistant to apoptosis inducers compared to proliferating cells. Resistance to death signals correlated with the level of protein kinase AKT phosphorylation. Skeletal muscle-derived cell lines can be multipurpose tools in therapy. Enhanced resistance of differentiated cells to certain types of damage shows their potential for long-term survival and maintenance in an organism. This article was published online on 29 January 2013. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 6 March 2013.

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“…Burdzinska et al [139] suggested a relationship between bone-marrow derived stem cells, multipotent muscle stem cells and satellite cells. In this proposed model, both MSC and HSC are released from the bone marrow to peripheral blood in response to certain stimuli such as exercise or skeletal muscle injury.…”

Section: Skeletal Musclementioning

confidence: 99%

Stem cell homing in musculoskeletal injury

2011

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The regenerative potential of injured adult tissue suggests the physiological existence of cells capable of participating in the reparative process. Recent studies indicate that stem-like cells residing in tissues contribute to tissue repair and are replenished by precursor bone marrowderived cells. Mesenchymal stromal cells (MSC) are among the candidates for reparative cells. These cells can potentially be mobilized into the circulation in response to injury signals and exert their reparative effects at the site of injury. Current therapies for musculoskeletal injuries pose unavoidable risks which can impede full recovery. Trafficking of MSC to the injury site and their subsequent participation in the regenerative process is thought to be a natural healing response that can be imitated or augmented by enhancing the endogenous MSC pool with exogenously administered MSC. Therefore, a promising alternative to the existing strategies employed in the treatment of musculoskeletal injuries is to reinforce the inherent reparative capacity of the body by delivering MSC harvested from the patient's own tissues to the site of injury. The aim of this review is to inform the reader of studies that have evaluated the intrinsic homing and regenerative abilities of MSC, with particular emphasis on the repair of musculoskeletal injuries. Research that supports the direct use of MSC (without in vitro differentiation into tissue-specific cells) will also be reported. Based on accruing evidence that the natural healing mechanism involves the recruitment of MSC and their subsequent reparative actions at the site of injury, as well as documented therapeutic response after the exogenous administration of MSC, the feasibility of the emerging strategy of instant stem-cell therapy will be proposed.

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Myogenic stem cells.

Cited by 13 publications

References 87 publications

The Mutual Interactions between Mesenchymal Stem Cells and Myoblasts in an Autologous Co-Culture Model

The Mutual Interactions between Mesenchymal Stem Cells and Myoblasts in an Autologous Co-Culture Model

Long‐term muscle‐derived cell culture: multipotency and susceptibility to cell death stimuli

Stem cell homing in musculoskeletal injury

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