Myostimulating effect of <i>Sesamum radiatum</i> aqueous leaf extract in isolated guinea-pig <i>Tainia caeci</i> contractile activity

Abstract

This study was carried to examine the effects of the aqueous leaf extract of Sesamum radiatum,a laxative plant on the contractile activity of Taenia caeci, an intestinal smooth muscle. Strips of Taenia caeci were rapidly removed from guinea-pig and were suspended between two L-shaped stainless steel hooks in a 10 ml organ bath with Mac Ewen solution. The isometric contractile force of the Taenia caeci strips were recorded by using a strain gauge. S. radiatum aqueous leaf extract (ESera) is a spasmogenic substance. This myostimulant effect is characterized by the increase of the rhythm and the amplitude of isolated guinea-pig Taenia caeci smooth muscle in normal solution and by the development of contracture in modified solution and in solution without calcium. A similar effect was observed with ACh which caused a graded increase of the contractile activity of Taenia caeci. The effects induced by ESera and ACh were reversed in the presence of atropine. The spasmogenic effect induced by ESera could justify partially the use of S. radiatum as laxative in traditional medicine.

Isolement par partition bio guidé du principe actif myostimulant de l’extrait aqueux de <i>Mareya micrantha</i> (Benth) Mull. Arg. (Euphorbiaceae)

“…La perte des effets myostimulants de l'extrait aqueux de Mareya micrantha dans le milieu physiologique sans calcium et en présence de l'EDTA, laisse entrevoir que ces principes actifs myostimulants sont principalement liés au calcium extracellulaires dont l'entrée provoquerait secondairement la libération du calcium interne. Ces résultats rappellent ceux de Sesamum radiatum (Pedaliaceae) (KONAN et al, 2011 (TRAORE et al, 2004), les effets myostimulants de F5 diminuent partiellement en présence de ATR à 1,15.10 -7 µg.ml -1 , suggérant ainsi qu'elle agirait en partie selon le même mécanisme que celui-ci. Autrement, les principes actifs de la fraction F5 augmenteraient l'amplitude de contraction du duodénum de lapin en mobilisant le calcium externe puis interne, via la stimulation des récepteurs cholinergiques.…”

Oral and Intraperitoneal Acute Toxicity Studies of Sesamum radiatum Aqueous Leaf Extract in Female Wistar Rats

“…The effect of plants depending on several factors including soil, geography, climate, pharmacological and toxicological studies have been undertaken on Sesamum radiatum harvested in Côte d'Ivoire [20][21][22][23][24] . It is in this context that the present work has been carried out in order to study the acute toxicity of the aqueous extract of the leaves of this medicinal plant in the female Wistar rat.…”