Myotropic activity of allatostatins in tenebrionid beetles

Lubawy, Jan; Marciniak, Paweł; Kuczer, Mariola; Rosiński, Grzegorz

doi:10.1016/j.npep.2018.05.003

Cited by 19 publications

(28 citation statements)

References 77 publications

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“…To analyze the changes in the contractile activity of the oviduct and hindgut treated with the tested compounds, a video microscopy technique was used [54,55]. Four-week-old insects of T. molitor beetle were anesthetized for 8 min.…”

Section: Methodsmentioning

confidence: 99%

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Spochacz

Chowański

Szymczak

et al. 2018

Toxins

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Background: Solanaceae plants produce glycoalkaloids (GAs) that affect various physiological processes of herbivorous insects and they are being tested as potential alternatives for synthetic pesticides. They cause lethal and sublethal effects. Nevertheless, their mode of action remains unclear. Therefore, we examined the effects of Solanum nigrum fruit extracts and pure glycoalkaloids on a model beetle, Tenebrio molitor. Methods: Plant extracts or pure alkaloids were added to the food of the larvae for three days. The lipid, glycogen, and protein content in the fat body and the midgut were determined, and the contractility of the heart, hindgut, and oviduct muscles was tested using the video-microscopy technique. Finally, the ultrastructure of the fat body and the midgut was observed using electron microscopy. Results: No lethal effects were noted. Sublethal changes were observed in the content of biomolecules, malformations of organelles, chromatin condensation, and heart and oviduct contractility. The observed effects differed between the tested glycoalkaloids and the extract. Conclusions: Both the extract and pure GAs have a wide range of effects that may result in impaired development, food intake, and reproduction. Some early effects may be used as bioindicators of stress. The effects of the extract and pure alkaloids suggest that the substances produced by the plant may act additively or synergistically.

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Section: Methodsmentioning

confidence: 99%

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Spochacz

Chowański

Szymczak

et al. 2018

Toxins

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show abstract

“…The pharmacological properties of cardioactive compounds can be easily tested using semi-isolated beetle hearts and the microdensitometric technique described by Gäde and Rosiński (1990). Confirmation of the usefulness of this study model and technique comes from the results obtained, for example, by Marciniak et al (2009, 2010a), Chowański and Rosiński (2017), Chowański et al (2017b), Lubawy et al (2018) and others. Our group used the heart of Z. atratus as a non-mammalian model to detect changes caused by drugs.…”

Section: Beetles As Model Organisms In Biomedicinementioning

confidence: 60%

“…In insects, two groups of neuropeptides are proven to be involved in the regulation of growth, metamorphosis and reproduction by regulation of production and secretion of the juvenile hormone (JH) by CA (Feyereisen, 1985), stimulatory allatotropins or inhibitory allatostatins. Among allatostatins, three separate families can be distinguished, which vary structurally (Lubawy et al, 2018). The most interesting of these families for biomedical research seems to be the so-called PISCF/ASTC allatostatins.…”

Section: Neuroendocrinologymentioning

confidence: 99%

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Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies – A Review

et al. 2019

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Model organisms are often used in biological, medical and environmental research. Among insects, Drosophila melanogaster, Galleria mellonella, Apis mellifera, Bombyx mori, Periplaneta americana , and Locusta migratoria are often used. However, new model organisms still appear. In recent years, an increasing number of insect species has been suggested as model organisms in life sciences research due to their worldwide distribution and environmental significance, the possibility of extrapolating research studies to vertebrates and the relatively low cost of rearing. Beetles are the largest insect order, with their representative – Tribolium castaneum – being the first species with a completely sequenced genome, and seem to be emerging as new potential candidates for model organisms in various studies. Apart from T. castaneum , additional species representing various Coleoptera families, such as Nicrophorus vespilloides, Leptinotarsa decemlineata, Coccinella septempunctata, Poecilus cupreus, Tenebrio molitor and many others, have been used. They are increasingly often included in two major research aspects: biomedical and environmental studies. Biomedical studies focus mainly on unraveling mechanisms of basic life processes, such as feeding, neurotransmission or activity of the immune system, as well as on elucidating the mechanism of different diseases (neurodegenerative, cardiovascular, metabolic, or immunological) using beetles as models. Furthermore, pharmacological bioassays for testing novel biologically active substances in beetles have also been developed. It should be emphasized that beetles are a source of compounds with potential antimicrobial and anticancer activity. Environmental-based studies focus mainly on the development and testing of new potential pesticides of both chemical and natural origin. Additionally, beetles are used as food or for their valuable supplements. Different beetle families are also used as bioindicators. Another important research area using beetles as models is behavioral ecology studies, for instance, parental care. In this paper, we review the current knowledge regarding beetles as model organisms and their practical application in various fields of life science.

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“…Peptide Trica-sNPF (SGRSPSLRLRFa) and its truncated form Trica-sNPF (4–11) (SPSLRLRFa) were synthesized according to the Fmoc procedure, as described previously (Lubawy et al 2018 ; Marciniak et al 2008 ). The purity of peptides was checked by HPLC and found to exceed 98% in each case.…”

Section: Methodsmentioning

confidence: 99%

Short neuropeptide F signaling regulates functioning of male reproductive system in Tenebrio molitor beetle

et al. 2020

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Neuropeptides of short neuropeptides F family (sNPF) have been identified in various arthropods. They are pleiotropic neuromolecules which so far have been mainly associated with regulation of feeding and metabolism, as well as growth and development, locomotion, circadian rhythm or learning and memory. Here, we describe the effects of Tenebrionid sNPF peptide (SGRSPSLRLRFa) on various aspects of the male reproductive physiology in the Tenebrio molitor beetle. We identified in silico the putative sNPF receptor Tenmo-sNPFR. Based on RT-PCR technique, it was shown that the receptor might be present in the male reproductive tissues of this beetle. The analysis of receptor amino acid sequence showed that it is similar to other beetle sNPFRs, as well as other insect species, and belongs rhodopsin-like G-protein-coupled receptors (GPCRs). Injections of Trica-sNPF and its shorter form Trica-sNPF (4-11) caused differentiated effects in T. molitor male reproductive tissues. After 24 h post injections, the peptides decreased the concentration of the soluble protein fraction in testes of 4-and 8-day-old beetles as well as the dry mass of these organs but only in 8-day-old individuals. The same effects were shown with regard to accessory glands. Both peptides decrease the concentration of the soluble protein fraction but do not affect the dry mass of this organ. Furthermore, injections of Trica-sNPF at the 10-7 M concentration decrease the total sperm number in the reproductive system. Surprisingly, the same concentration of the shorter form, Trica-sNPF (4-11) increased the sperm number. It was also shown that both peptides in different manner influence contractions of ejaculatory duct. The data presented in this article give new evidence that sNPFs are involved in the regulation of reproductive events in beetles, which might be the part of a larger neuropeptide network combining feeding, growth and development with the physiology of reproduction.

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Myotropic activity of allatostatins in tenebrionid beetles

Cited by 19 publications

References 77 publications

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Beetles as Model Organisms in Physiological, Biomedical and Environmental Studies – A Review

Short neuropeptide F signaling regulates functioning of male reproductive system in Tenebrio molitor beetle

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