Myricitrin Degraded by Simulated Digestion Inhibits Oxidation of Human Low-Density Lipoprotein

Effects of Uptake of Flavonoids on Oxidative Stress Induced by Hydrogen Peroxide in Human Intestinal Caco-2 Cells

“…6) Previously we reported that myricitrin suppressed low-density lipoprotein (LDL) oxidation due to 2,2 0 -azobis (2-amidinopropane) dihydrochloride (AAPH) radical scavenging and copper ion chelation. 7) On the other hand, the pro-oxidative effects and cytotoxicity of flavonoids, possibly due to generation of ROS, including H 2 O 2 , and induction of apoptosis have been reported. [8][9][10] Thus flavonoids appear to have both antioxidative and pro-oxidative effects, but clear evidence of prevention of oxidative stress by the antioxidative activity of flavonoids is lacking.…”

Transepithelial Permeability of Myricitrin and Its Degradation by Simulated Digestion in Human Intestinal Caco-2 Cell Monolayer

“…To investigate the effect of degradation on Caco-2 cell monolayer permeability, we prepared DM by simulated digestion. 5) Mildly alkaline-treated myricitrin produced certain components, as shown in Fig. 3.…”

“…4) Previously, we found that myricitrin and its alkaline degradation products (DM) strongly inhibited LDL oxidation. 5) These findings suggest that the intake of myricitrin-rich foods might help inhibit harmful oxidation in vivo. Degraded myricitrin was prepared by simulated intestinal digestion in a mildly alkaline solution (pH 8.5, 60 min) and analyzed by HPLC/MS.…”

“…[2][3][4] Myricitrin and its alkaline degradation products (DM) prepared by mild alkaline treatment that simulated the human intestinal environment has a strong inhibitory effect on low-density lipoprotein (LDL) oxidation due to radical-scavenging and metal-ion chelating activities. 5) Several flavonoids have recently been detected in human blood after oral intake, and the absorption mechanism of flavonoids has also been studied in vitro using cell lines. [6][7][8] It is important to understand the mechanism of intestinal absorption of myricitrin in order to develop a method of increasing the bioavailability of myricitrin.…”

Enzymatic Production of Highly Soluble Myricitrin Glycosides Using β-Galactosidase

“…10) Moreover, myricitrin has recently been reported to inhibit the oxidation of low-density lipoprotein. 11) Myricitrin could be a useful anti-oxidative ingredient, but its range of use has been limited because of its low water solubility. Meanwhile, transglycosylation by enzymes from various bacteria has been used to improve such the physiochemical properties as the solubility in water, taste and oxidative stability of various compounds.…”