Transepithelial Permeability of Myricitrin and Its Degradation by Simulated Digestion in Human Intestinal Caco-2 Cell Monolayer

Yokomizo, Atsushi; Moriwaki, Masamitsu

doi:10.1271/bbb.69.1774

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…2001; Boyer et al. 2005; Yokomizo and Moriwaki 2005). The objectives of this study were to assess the gastrointestinal stability and absorbability of bioactive components (aloin, aloe‐emodin and aloenin) in aloe using an in vitro digestion model with caco‐2 cell, as well as to determine the possible mechanism of intestinal transport of two bioactive components (aloin and aloenin A).…”

Section: Introductionmentioning

confidence: 99%

“…The bioavailability of bioactive components in plants is mainly evaluated using animals or human subjects, which is a complex, expensive, and ethically problematic method. Hence, in this study, we used an in vitro digestion model simulation of salivary, gastric and intestinal phases combined with human intestinal cell line (caco-2 cell), which has been well established to predict the bioavailability of nutrients or phenolic compounds in food system or plants 426 S.-M. SHIM and H. KWON (Ferruzzi et al 2001;Boyer et al 2005;Yokomizo and Moriwaki 2005). The objectives of this study were to assess the gastrointestinal stability and absorbability of bioactive components (aloin, aloe-emodin and aloenin) in aloe using an in vitro digestion model with caco-2 cell, as well as to determine the possible mechanism of intestinal transport of two bioactive components (aloin and aloenin A).…”

Section: Introductionmentioning

confidence: 99%

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ASSESSING ABSORBABILITY OF BIOACTIVE COMPONENTS IN ALOE USINGIN VITRODIGESTION MODEL WITH HUMAN INTESTINAL CELL

Shim¹,

Kwon

2010

Journal of Food Biochemistry

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This study used a simulated in vitro digestion model coupled with caco‐2 cell to assess the digestive stability and absorption of aloin, aloe‐emodin and aloenin A. Aloenin A and aloe‐emodin were stable and entirely recovered during simulated digestion, but 50% of aloin was lost. Approximately 53.2, 7.3 and 28.7% of aloe‐emodin, aloenin A and aloin, respectively, was transported into both apical and basolateral compartments after 1 h incubation in caco‐2 cell. The involvement of several transporter proteins for aloin and aloenin A was examined. An inhibitor of SGLT1 on apical surface (phloridzin) or that of GLUT2 on basolateral membrane (cytochalasin B) reduced the absorption of aloin by 40 or 60%, respectively, indicating that aloin is likely to be a partial substrate of SGLT1. In the presence of an efflux transporter inhibitor (verapamil), the transport of aloenin A through an intentinal apical membrane increased up to 2.1 times compared with the control (without verapamil). PRACTICAL APPLICATIONS Our results on both digestive stability and intestinal absorption characteristics of bioactive components in aloe could be of helpful information for promoting its bioavailability. The in vitro technique described in this study provides a rapid and cost‐effective alternative for predicting bioavailability of biomarkers in aloe functional food.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ASSESSING ABSORBABILITY OF BIOACTIVE COMPONENTS IN ALOE USINGIN VITRODIGESTION MODEL WITH HUMAN INTESTINAL CELL

Shim¹,

Kwon

2010

Journal of Food Biochemistry

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“…15) The human colon adenocarcinoma cell line, Caco-2, has been used as a model to study the intestinal absorption or secretion of various drugs. 16) This cell line spontaneously differentiates during culture into polarized cells with many enterocyte-like properties of transportrelated epithelia. Caco-2 cells retain various transporters expressed in the intestines such as P-glycoprotein, MRPs and SGLT 1 .…”

mentioning

confidence: 99%

Cellular Absorption of Anthraquinones Emodin and Chrysophanol in Human Intestinal Caco-2 Cells

Teng¹,

Zhou²,

Ran³

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…Given that only limited biological properties or functional activities have been worked out for each natural product, there still has a “long way to go” for fully identifying the biological activities of natural products . Myricitrin is a naturally occurring flavonoid derived from bayberry bark and fruit, which has been reported to exhibit numerous biological activities such as antioxidative, anti‐inflammatory and antinociceptive effects . Despite the biological activities, myricitrin is relatively easy to extract and purify …”

Section: Discussionmentioning

confidence: 99%

Inhibition effects of a natural inhibitor on RANKL downstream cellular signalling cascades cross‐talking

Wang

Hao

Zhang

et al. 2018

J Cellular Molecular Medi

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Myricitrin is a natural occurring flavonoid glycoside that possesses effects on inhibiting nitric oxide (NO) transmission and preventing inflammatory reaction. Although previous study showed the myricitrin possesses antibone loss effects via reducing the expression of IL‐6 and partially suppressing reactive oxygen species (ROS) production. However, the effects of myricitrin on nuclear factor‐kappaB ligand (RANKL)‐stimulated osteoclastogenesis have not yet been further investigated. The current study was aimed to demonstrating the inhibitory effects of myricitrin on RANKL‐stimulated osteoclastogenesis and relevant mechanisms. We found myricitrin significantly suppressed osteoclastogenesis suggesting that it may acts on RANKL/RANK induced downstream signal cross cascading in osteoclast precursors. In that, our Western blotting results showed myricitrin significantly attenuated RNAKL/MAPKs (phosphorylation of p38, ERK, JNK) and AKT signal cascading. Complementing previous study, our results suggesting as a natural inhibitor, myricitrin possesses the potential therapeutic effects on inflammatory osteolysis.

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Transepithelial Permeability of Myricitrin and Its Degradation by Simulated Digestion in Human Intestinal Caco-2 Cell Monolayer

Cited by 9 publications

References 13 publications

ASSESSING ABSORBABILITY OF BIOACTIVE COMPONENTS IN ALOE USINGIN VITRODIGESTION MODEL WITH HUMAN INTESTINAL CELL

ASSESSING ABSORBABILITY OF BIOACTIVE COMPONENTS IN ALOE USINGIN VITRODIGESTION MODEL WITH HUMAN INTESTINAL CELL

Cellular Absorption of Anthraquinones Emodin and Chrysophanol in Human Intestinal Caco-2 Cells

Inhibition effects of a natural inhibitor on RANKL downstream cellular signalling cascades cross‐talking

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