N-3 Polyunsaturated fatty acid ethyl esters decrease the invasion, but not the proliferation, of human colorectal cancer cells via a PI3K-dependent mechanism in vitro

Pfister, Eric; Smith, Rebecca; Lane, Michelle A.

doi:10.1016/j.plefa.2021.102273

Cited by 6 publications

(8 citation statements)

References 43 publications

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“…Because members of our group use HCT-116 cell line to examine the effect of diet-derived compounds on cell invasion [ 47 , 48 ] and previous work examined the ability of LYC to decrease the invasion of the HCT-116 cell line (wild type) and the LoVo models [ 38 ], we focused on these models. We first evaluated whether treatment for 1, 5, 8 and 24 h with HAE, LYC, or NAR affected cell adhesion at three concentrations: one close to their mean IC 50 , one two times higher, and one two times lower in cells cultured with 10% FBS ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Importantly, considering the lack of adhesion of cells in presence of the compounds as described above, we corrected the invasion rates to reflect the number of actual adherent cells measured in wells seeded in parallel. Correcting cell invasion for adherent cells allowed us to isolate the effects of these compounds on invasion, exclusive of their impact on cell viability, proliferation, and adhesion [ 47 , 48 ]. Figure 4 A,B illustrate the percentage of adherent HCT-116 and LoVo cells 24 h after their seeding in parallel to the Boyden chambers, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Prior to seeding HCT-116 cells in the invasion chambers, the medium was removed and cells were washed twice with phosphate buffered saline and incubated in DMEM lacking FBS for 48 h before seeding at a density of 1 × 10 5 cells/well into the upper portion of Matrigel-coated invasion chambers (Corning Biocoat, Corning, NY, USA), as described previously by our group in [ 47 , 48 ]. The upper portion of each chamber contained cells suspended in serum-free DMEM containing DMSO vehicle alone or increasing concentrations of the Amaryllidaceae alkaloids NAR, LYC, and HAE.…”

Section: Methodsmentioning

confidence: 99%

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Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

et al. 2022

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Alkaloids isolated from members of the Amaryllidaceae plant family are promising anticancer agents. The purpose of the current study was to determine if the isocarbostyrils narciclasine, pancratistatin, lycorane, lycorine, crinane, and haemanthamine inhibit phenomena related to cancer progression in vitro. To achieve this, we examined the proliferation, adhesion, and invasion of cultured human colon cancer cells via MTT assay and Matrigel-coated Boyden chambers. In addition, Luminex assays were used to quantify the secretion of matrix metalloproteinases (MMP) and cytokines associated with poor clinical outcomes. We found that all alkaloids decreased cell proliferation regardless of TP53 status, with narciclasine exhibiting the greatest potency. The effects on cell proliferation also appear to be specific to cancer cells. Narciclasine, lycorine, and haemanthamine decrease both adhesion and invasion but with various potencies depending on the cell line. In addition, narciclasine, lycorine, and haemanthamine decreased the secretion of MMP-1, -2, and -7, as well as the secretion of the cytokines pentraxin 3 and vascular endothelial growth factor. In conclusion, the present study shows that Amaryllidaceae alkaloids decrease phenomena and cytokines associated with colorectal cancer progression, supporting future investigations regarding their potential as multifaceted drug candidates.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

et al. 2022

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“…It is noteworthy that although most FAMEs exhibited higher levels in white sesame seeds, n-3 poly-unsaturated FAMEs, including C18:3n3 and C20:5n3, were more abundant in black sesame seeds. Several reports have found that n-3 long chain poly-unsaturated fatty acids (LC-PUFA) are involved in many important biological activities, for example, anti-inflammation [ 29 ], anti-cancer [ 30 ], anti-aging [ 31 ], and lowering the risk of cardiovascular diseases [ 32 ]. It was hypothesized that the expression levels of genes associated with LC-PUFA synthesis could be varied between the two groups of sesame seeds.…”

Section: Resultsmentioning

confidence: 99%

Discrimination of Black and White Sesame Seeds Based on Targeted and Non-Targeted Platforms with Chemometrics: From Profiling towards Identification of Chemical Markers

Wang

Zhang

et al. 2022

Foods

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The present study was conducted to clarify the differences in the multi-element, volatile organic compound, fatty acid, and metabolite fingerprints between black and white sesame seeds. A total of 53 chemical elements, 32 volatile flavor compounds, 40 fatty acids, and 283 metabolites were identified and evaluated in the two groups of sesame seeds. Univariate and multivariate statistics indicated a distinct separation between the two groups of sesame seeds. A panel of 16 chemical elements, 3 volatile compounds, 8 individual fatty acids, and 54 metabolites with p value < 0.05 and variable importance in projection score > 1 were selected as the most important discriminants for the two types of sesame seeds. Overall, these data reveal the influence of genotype on the chemical composition of sesame seeds. Our findings also demonstrate that the hybrid model of instrumental analysis and chemometrics is feasible for the discrimination of black and white sesame seeds.

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“…The results showed the ability of n-3 PUFA ethyl esters to inhibit PI3K activity confers their potency to reduce CRC cell invasion, but not proliferation. ( 311 ) Furthermore, n-3 PUFAs can activate pro-apoptotic signaling by interacting with G protein-coupled receptors (GPCRs), resulting in an anti-CRC activity. Non-epithelial cells including adipocytes and macrophages have been found to express these GPCRs.…”

Section: Selected Components Of the Mediterranean Diet And Their Effe...mentioning

confidence: 99%

Chemoprevention effect of the Mediterranean diet on colorectal cancer: Current studies and future prospects

et al. 2022

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Colorectal cancer (CRC) is the third most common cancer and the second most deadly cancer worldwide. Nevertheless, more than 70% of CRC cases are resulted from sporadic tumorigenesis and are not inherited. Since adenoma-carcinoma development is a slow process and may take up to 20 years, diet-based chemoprevention could be an effective approach in sporadic CRC. The Mediterranean diet is an example of a healthy diet pattern that consists of a combination of nutraceuticals that prevent several chronic diseases and cancer. Many epidemiological studies have shown the correlation between adherence to the Mediterranean diet and low incidence of CRC. The goal of this review is to shed the light on the anti-inflammatory and anti-colorectal cancer potentials of the natural bioactive compounds derived from the main foods in the Mediterranean diet.

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N-3 Polyunsaturated fatty acid ethyl esters decrease the invasion, but not the proliferation, of human colorectal cancer cells via a PI3K-dependent mechanism in vitro

Cited by 6 publications

References 43 publications

Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

Discrimination of Black and White Sesame Seeds Based on Targeted and Non-Targeted Platforms with Chemometrics: From Profiling towards Identification of Chemical Markers

Chemoprevention effect of the Mediterranean diet on colorectal cancer: Current studies and future prospects

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