N‐acetylation phenotyping using dapsone in a Jordanian population.

Abstract

1 The N-acetylation of dapsone (DDS) was studied in 160 unrelated healthy Jordanian volunteers. 2 The frequency of slow acetylators determined using the plasma monoacetyldapsone (MADDS) to DDS ratio (MADDS/DDS), was 67.5% with a 95% confidence interval of 59 to 76%. Slow acetylators had an acetylation ratio of < 0.42. 3 Applying the Hardy-Weinberg Law, the frequency of the recessive allele controlling slow acetylation was found to be 0.82 ± 0.02. 4 The frequency distribution histogram of the plasma MADDS/DDS ratio showed an apparent trimodal pattern. The number of homozygous (n = 16) and heterozygous (n = 36) rapid acetylators derived from the observed data did not agree with those predicted for the respective rapid acetylators (n = 5 and n = 47) according to the Hardy-Weinberg Law. The suggested antimode used to discriminate the two groups was 0.82. 5 The mean plasma concentration of MADDS and the mean plasma acetylation ratio were about three times lower in slow than in rapid acetylators. However, there was no difference in mean plasma DDS concentration between slow and rapid acetylators. 6 There was a significant correlation (r = 0.853, P < 0.001) between plasma MADDS concentration and the acetylation ratio. For DDS such a correlation was absent (r = 0.059, P = 0.23).

Sequence analysis of theN-acetyltransferase 2 gene (NAT2) among Jordanian volunteers

“…This high frequency of NAT2 slow encoding genotype was also reported previously among Jordanians, which could explain the high proportion of slow acetylation phenotype found in Jordanians [39]. …”

Acetylator phenotypes of Jordanian diabetics

“…Plasma was separated immediately by centrifugation at 3000 rpm and was stored at -20 °C pending analysis. Dapsone and monoacetyldapsone in plasma were measured by HPLC [12,13].…”

Effects of Dietary Broccoli on Human in Vivo Caffeine Metabolism: A Pilot Study on a Group of Jordanian Volunteers

“…Few studies on drug metabolism have been conducted on the Jordanian population. These studies examined the N-acetylation phenotyping using dapsone [18], acetylator phenotypes in Jordanian diabetics [19], S-mephenytoin hydroxylation which is considered as a standard CYP2C19 phenotyping probe [20,21] and genetic polymorphism of CYP2D6 [22,23] and CYP2A6 activities in a Jordanian population [24]. Jordanian population has not been screened for CYP activities or the effect of food on these activities.…”