N‐dodecylated chitosan/graphene oxide composite cryogel for hemostasis and antibacterial treatment

Abstract

Uncontrolled massive bleeding remains a global issue, which requires an exigent response for safe and efficient hemostat. However, most of hemostats without antimicrobial property could trigger serious bacterial infection problem. Herein, a composite cryogel was synthesized by double bond N-dodecylated chitosan (DCSG) and graphene oxide (GO). The cryogel (DCSG/GO) with macroporous structure exhibited high-fluid adsorption ability, excellent mechanical property, and fluid-triggered shape recovery performance. Moreover, the in vitro blood coagulation experiments proved that DCSG/ GO 5% possessed better coagulation efficiency than medical gelatin sponge. And the DCSG/GO 5% cryogel exhibited more erythrocyte and platelets activation. Besides, the cryogel had excellent near-infrared (NIR)-assisted photothermal antibacterial activity against both Escherichia coli and Staphylococcus aureus and killed 99% of them after 20 min NIR irradiation. In addition, the vitro hemolysis and cytotoxicity results proved the well biocompatibility of cryogel. Therefore, the biocompatible DCSG/GO cryogel has great potential application prospect in clinical hemorrhage control and anti-infection treatment.

Recent Advances of Chitosan-Based Hydrogels for Skin-Wound Dressings

“…Therefore, alkylated chitosan-based hydrogel dressings have significant advantages in hemostasis applications. Qiu et al [ 80 ] prepared composite hydrogels from double-bonded modified N-dodecylchitosan (DCSG), polyethylene glycol diacrylate (PEGDA), and graphene oxide (GO). The prepared hydrogels showed excellent near-infrared assisted photothermal antibacterial properties against E. coli and S. aureus .…”

Design and Properties of Antimicrobial Biomaterials Surfaces

“…[ 83 ] Similarly, modification with double bond N ‐dodecylated or quaternized chitosan produces the GO derivative with photothermal antibacterial capability and biocompatibility. [ 300,301 ] The reactive carboxyl and hydroxyl groups on GO provide crosslinking sites to connect with macromolecules. In the cellulose‐GO nanosheet hydrogel network, the GO nanosheets not only act as a photothermal agent to sterilize bacteria upon NIR irradiation, but also strengthen the mechanical properties of the hydrogel by absorbing and dissipating energy due to the rigid characteristics.…”