N-glycan transition of the early developmental stage in Oryza sativa

Horiuchi, Risa; Hirotsu, Naoki; Miyanishi, Nobumitsu

doi:10.1016/j.bbrc.2016.06.082

Cited by 4 publications

(7 citation statements)

References 19 publications

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“…2 ). As a result of reversed phase HPLC, two major peaks were detected; as a result of N -glycan two-dimensional mapping, the retention time of peak G2 corresponded to that of known position N -glycan, GN2M3FX 20 , and the other one was eluted at around 3 min (Fig. 5A , peak G1).…”

Section: Resultsmentioning

confidence: 99%

“…N -Glycans were prepared by hydrazinolysis, N -acetylation. The reducing ends of the liberated N -glycans were then tagged with a fluorophore, 2-aminopyridyne (pyridylaminated N -glycans; PA- N -glycans), as described in previous paper 20 . These preparations were performed following details in Hase et al .…”

Section: Methodsmentioning

confidence: 99%

“…The detection of PA- N -glycans performed by use of fluorescence spectrophotometer at 315 nm excitation and 400 nm emission. Each HPLC conditions were described in previous paper 20 .…”

Section: Methodsmentioning

confidence: 99%

“…MALDI-TOF mass spectrometry analysis was then performed using an AXIMA resonance instrument (Shimadzu) in reflector mode. Sample preparation was described in previous paper 20 .…”

Section: Methodsmentioning

confidence: 99%

“…A two picomoles of PA- N -glycans was prepared in 1 microL of accessory reaction buffer (5 mM CaCl 2 , 10 mM ammonium acetate buffer, pH 4.5) and 2 microL of D. D. W, 1 microL of beta- N- acetylhexosaminidase (0.05 units/microL) was added, and the mixture was incubated at 37 °C for 4 h, and then 1 microL of jack bean alpha-mannosidase (19 units/mg) and 5 microL of 10 mM ammonium acetate buffer (pH 4.5) were added, and the mixture was incubated at 37 °C for 1 h. alpha-Mannosidase digestion was described in previous paper 20 . To stop all reactions, the mixtures were boiled for 5 min at 95 °C, and the mixture was analyzed by size-fractionation HPLC.…”

Section: Methodsmentioning

confidence: 99%

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Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Horiuchi

Nakajima

Kashiwada

et al. 2018

Sci Rep

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Silver nanomaterials have been mainly developed as antibacterial healthcare products worldwide, because of their antibacterial activity. However, there is little data regarding the potential risks and effects of large amounts of silver nanomaterials on plants. In contrast, N-glycans play important roles in various biological phenomena, and their structures and expressions are sensitive to ambient environmental changes. Therefore, to assesse the effects of silver nanomaterials, we focused on the correlation between N-glycans and the effects of silver nanomaterials in plants and analyzed N-glycan structures in Oryza sativa seedlings exposed to silver nanocolloids (SNCs). The phenotype analysis showed that the shoot was not affected by any SNC concentrations, whereas the high SNC exposed root was seriously damaged. Therefore, we performed comparative N-glycan analysis of roots. As a result, five of total N-glycans were significantly increased in SNC exposed roots, of which one was a free-N-glycan with one beta-N-acetylglucosamine residue at the reducing end. Our results suggest that the transition of plant complex type N-glycans, including free-N-glycans, was caused by abnormalities in O. sativa development, and free-N-glycan itself has an important role in plant development. This study originally adapted glycome transition analysis to environmental toxicology and proposed a new category called “Environmental glycobiology”.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The detection of PA- N -glycans performed by use of fluorescence spectrophotometer at 315 nm excitation and 400 nm emission. Each HPLC conditions were described in previous paper 20 .…”

Section: Methodsmentioning

confidence: 99%

“…MALDI-TOF mass spectrometry analysis was then performed using an AXIMA resonance instrument (Shimadzu) in reflector mode. Sample preparation was described in previous paper 20 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Horiuchi

Nakajima

Kashiwada

et al. 2018

Sci Rep

Self Cite

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Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Harvey

2021

Mass Spectrometry Reviews

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This review is the ninth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.

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Structural analysis of N-glycans in medaka gut exposed to silver and titanium dioxide nanoparticles

Horiuchi

Ozawa

Tomii

et al. 2021

Environ Sci Pollut Res

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N-glycan transition of the early developmental stage in Oryza sativa

Cited by 4 publications

References 19 publications

Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Effects of silver nanocolloids on plant complex type N-glycans in Oryza sativa roots

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Structural analysis of N-glycans in medaka gut exposed to silver and titanium dioxide nanoparticles

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