N-Glycosylation process in both ER and Golgi plays pivotal role in plant immunity

Kang, Byeong Sul; Baek, Ji Hyeong; Macoy, Donah Mary; Chakraborty, Rupak; Cha, Joon‐Yung; Hwang, Duk‐Ju; Lee, Yeon Hee; Lee, Sang Yeol; Kim, Woe‐Yeon; Kim, Min Gab

doi:10.1007/s12374-015-0197-3

Cited by 18 publications

(7 citation statements)

References 40 publications

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“…Recently, it has been shown that N-glycans attached to ectodomains of plasma membrane pattern recognition receptors (PRR) likely form initial contact sites between plant cells and invading pathogens 60 . The N-glycosylation of membrane proteins, both in the ER and in the Golgi apparatus, has been shown to play a crucial role in plant immunity 61 , 62 .…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae

Assis

Polloni

Patané

et al. 2017

Sci Rep

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The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect different hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on identifying proteins enriched in phytopathogens that could explain the lifestyle and the ability to infect plants. Using a computational approach, we identified seven phytopathogen-enriched protein families putatively secreted by type II secretory system: PheA (CM-sec), LipA/LesA, VirK, and four families involved in N-glycan degradation, NixE, NixF, NixL, and FucA1. In silico and phylogenetic analyses of these protein families revealed they all have orthologs in other phytopathogenic or symbiotic bacteria, and are involved in the modulation and evasion of the immune system. As a proof of concept, we performed a biochemical characterization of LipA from Xac306 and verified that the mutant strain lost most of its lipase and esterase activities and displayed reduced virulence in citrus. Since this study includes closely related organisms with distinct lifestyles and highlights proteins directly related to adaptation inside plant tissues, novel approaches might use these proteins as biotechnological targets for disease control, and contribute to our understanding of the coevolution of plant-associated bacteria.

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Section: Discussionmentioning

confidence: 99%

Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae

Assis

Polloni

Patané

et al. 2017

Sci Rep

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“…Besides, PNG has been well established as playing multiple roles in regulating the stress tolerance of plants. It is important for the transport of the secretory proteins that are involved in plant adaptive responses to environmental stresses, such as plant immunity [44], temperature tolerance [45], and salt sensitivity [46,47]. Of late, the effects and mechanism of N-glycosylation on photosynthesis have been elucidated [48].…”

Section: Function Of N-glycosylation In Plantsmentioning

confidence: 99%

(De)Activation (Ir)Reversibly or Degradation: Dynamics of Post-Translational Protein Modifications in Plants

Muleya

Lois

Chahtane

et al. 2022

Life

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The increasing dynamic functions of post-translational modifications (PTMs) within protein molecules present outstanding challenges for plant biology even at this present day. Protein PTMs are among the first and fastest plant responses to changes in the environment, indicating that the mechanisms and dynamics of PTMs are an essential area of plant biology. Besides being key players in signaling, PTMs play vital roles in gene expression, gene, and protein localization, protein stability and interactions, as well as enzyme kinetics. In this review, we take a broader but concise approach to capture the current state of events in the field of plant PTMs. We discuss protein modifications including citrullination, glycosylation, phosphorylation, oxidation and disulfide bridges, N-terminal, SUMOylation, and ubiquitination. Further, we outline the complexity of studying PTMs in relation to compartmentalization and function. We conclude by challenging the proteomics community to engage in holistic approaches towards identification and characterizing multiple PTMs on the same protein, their interaction, and mechanism of regulation to bring a deeper understanding of protein function and regulation in plants.

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“…Overexpression of the gene At2g02360 in Arabidopsis enhances resistance to Pst DC3000 ( Stefanowicz et al., 2016 ). Arabidopsis mutants defective in N -glycosylation, namely staurosporine- and temperature-sensitive 3a and 3b ( stt3a and stt3b ) of oligosaccharyl transferase subunit and complex glycan 1 ( cgl1 ) in Golgi apparatus, displayed alternated plant immunity ( Kang et al., 2015 ). Compared with the wild-type plant, the mutant stt3a is more susceptible against Pst and Erwinia carotovora subsp.…”

Section: Crosstalk Of Glycosylation and Ubiquitinationmentioning

confidence: 99%

Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

Zhang

Zeng

2020

Plant Communications

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Post-translational modifications (PTMs) are central to the modulation of protein activity, stability, subcellular localization, and interaction with partners. They greatly expand the diversity and functionality of the proteome and have taken the center stage as key players in regulating numerous cellular and physiological processes. Increasing evidence indicates that in addition to a single regulatory PTM, many proteins are modified by multiple different types of PTMs in an orchestrated manner to collectively modulate the biological outcome. Such PTM crosstalk creates a combinatorial explosion in the number of proteoforms in a cell and greatly improves the ability of plants to rapidly mount and fine-tune responses to different external and internal cues. While PTM crosstalk has been investigated in depth in humans, animals, and yeast, the study of interplay between different PTMs in plants is still at its infant stage. In the past decade, investigations showed that PTMs are widely involved and play critical roles in the regulation of interactions between plants and pathogens. In particular, ubiquitination has emerged as a key regulator of plant immunity. This review discusses recent studies of the crosstalk between ubiquitination and six other PTMs, i.e., phosphorylation, SUMOylation, poly(ADP-ribosyl)ation, acetylation, redox modification, and glycosylation, in the regulation of plant immunity. The two basic ways by which PTMs communicate as well as the underlying mechanisms and diverse outcomes of the PTM crosstalk in plant immunity are highlighted.

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N-Glycosylation process in both ER and Golgi plays pivotal role in plant immunity

Cited by 18 publications

References 40 publications

Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae

Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae

(De)Activation (Ir)Reversibly or Degradation: Dynamics of Post-Translational Protein Modifications in Plants

Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

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