N-Hydroxy-4-(4-chlorophenyl)thiazole-2(3H)-thione as a Photochemical Hydroxyl-radical Source: Photochemistry and Oxidative Damage of DNA (Strand Breaks) and 2′-Deoxyguanosine (8-oxodG Formation)¶

Adam, Waldemar; Hartung, Jens; Okamoto, Hideki; Saha‐Möller, Chantu R.; Špehar, Kristina

doi:10.1562/0031-8655(2000)0720619nhctht2.0.co2

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…In the present work, we used the atomic force microscopy (AFM) to investigate the hydroxyl radical induced DNA damage and the effects of NAC by imaging DNA molecules that were exposed to free radicals and treated with NAC under various conditions. Most previously reported experimental results on the assessment of DNA strand breaks were obtained using gel electrophoresis and other techniques that measured the average values of the extent of damages [22][23][24][25]. AFM measurements can provide the average value, as well as the distribution of double-strand breaks among the DNA molecules as Abbreviations: AFM, atomic force microscopy; NAC, N-acetylcysteine individual molecules can be visualized and quantified in the AFM images.…”

Section: Introductionmentioning

confidence: 99%

Quantitative measurement of hydroxyl radical induced DNA double‐strand breaks and the effect of N‐acetyl‐l‐cysteine

Yang

2006

FEBS Letters

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Reactive oxygen species, such as hydroxyl or superoxide radicals, can be generated by exogenous agents as well as from normal cellular metabolism. Those radicals are known to induce various lesions in DNA, including strand breaks and base modifications. These lesions have been implicated in a variety of diseases such as cancer, arteriosclerosis, arthritis, neurodegenerative disorders and others. To assess these oxidative DNA damages and to evaluate the effects of the antioxidant N-acetyl-L L-cysteine (NAC), atomic force microscopy (AFM) was used to image DNA molecules exposed to hydroxyl radicals generated via Fenton chemistry. AFM images showed that the circular DNA molecules became linear after incubation with hydroxyl radicals, indicating the development of double-strand breaks. The occurrence of the double-strand breaks was found to depend on the concentration of the hydroxyl radicals and the duration of the reaction. Under the conditions of the experiments, NAC was found to exacerbate the free radical-induced DNA damage.

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Section: Introductionmentioning

confidence: 99%

Quantitative measurement of hydroxyl radical induced DNA double‐strand breaks and the effect of N‐acetyl‐l‐cysteine

Yang

2006

FEBS Letters

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Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

et al. 2008

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Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability.

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A Radical Version of the Bromo‐ and the Iodocyclization of Bis(homoallylic) Alcohols — The Synthesis of Halogenated Tetrahydrofurans by Stereoselective Alkoxyl Radical Ring Closures

et al. 2003

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A new synthesis of bromo-and iodomethyl-substituted tetrahydrofurans has been devised. The sequence starts with the conversion of aryl-functionalized bis(homoallylic) alcohols 1 into N-alkenoxythiazole-2(3H)-thiones 6 or pyridine-2(1H)-thiones 7. When photolyzed in the presence of appropriate trapping reagents, thiones 6 and 7 efficiently liberated substituted 4-penten-1-oxyl radicals 2, which underwent synthetically useful 5-exo-trig cyclizations. Cyclized radicals 3 were trapped with BrCCl 3 or an adequate iodine atom donor (either n-C 4 F 9 I or diethyl 2-iodo-2-methyl malonate) to provide halocyclization products 4 or 5. This strategy has been applied for the synthesis of 3-, 4-, or 5-phenyl-substituted 2-(1-bromo-1-methylethyl)tetrahydrofurans 4a−c (75−90%, 36−96% de), which were not attainable as major products

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N-Hydroxy-4-(4-chlorophenyl)thiazole-2(3H)-thione as a Photochemical Hydroxyl-radical Source: Photochemistry and Oxidative Damage of DNA (Strand Breaks) and 2′-Deoxyguanosine (8-oxodG Formation)¶

Cited by 6 publications

References 33 publications

Quantitative measurement of hydroxyl radical induced DNA double‐strand breaks and the effect of N‐acetyl‐l‐cysteine

Quantitative measurement of hydroxyl radical induced DNA double‐strand breaks and the effect of N‐acetyl‐l‐cysteine

Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

A Radical Version of the Bromo‐ and the Iodocyclization of Bis(homoallylic) Alcohols — The Synthesis of Halogenated Tetrahydrofurans by Stereoselective Alkoxyl Radical Ring Closures

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