N-lauryltyramine capped copper nanoparticles exhibit a selective colorimetric response towards hazardous mercury(<scp>ii</scp>) ions and display true anti-biofilm and efflux pump inhibitory effects in E. coli

Megarajan, Sengan; Vidhyalakshmi, Mohan; Ahmed, Khawaja Ashfaque; Murali, V.; Niranjani, Bommasamudram R. S.; Nagarajan, Saisubramanian; Veerappan, Anbazhagan

doi:10.1039/c6ra16912k

Cited by 20 publications

(9 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, nanoparticles exhibit the highest antibacterial and biofilm inhibition, indicating that these two activities are primarily related to O 2 •– generation . However, biofilm inhibition is found to be several-fold less than antibacterial efficiency, in line with reports that materials with simultaneous antibacterial and biofilm inhibition may not be effective against mature biofilms …”

Section: Results and Discussionsupporting

confidence: 83%

“…72 However, biofilm inhibition is found to be several-fold less than antibacterial efficiency, in line with reports that materials with simultaneous antibacterial and biofilm inhibition may not be effective against mature biofilms. 73 DPPH Scavenging by MgO Nanostructures. MgO nanostructures are known to have significant O 2…”

Section: Mgo Can Impart Oxidative Stress Via Production Of Omentioning

confidence: 99%

See 1 more Smart Citation

Effect of Morphology and Concentration on Crossover between Antioxidant and Pro-oxidant Activity of MgO Nanostructures

et al. 2018

View full text Add to dashboard Cite

The toxicity of nanomaterials can sometimes be attributed to photogenerated reactive oxygen species (ROS), but these ROS can also be scavenged by nanomaterials, yielding opportunities for crossover between the properties. The morphology of nanomaterials also influences such features due to defect-induced properties. Here we report morphology-induced crossover between pro-oxidant activity (ROS generation) and antioxidant activity (ROS scavenging) of MgO. To study this process in detail, we prepared three different nanostructures of MgO (nanoparticles, nanoplates, and nanorods) and characterized them by HRTEM. These three nanostructures effectively generate superoxide anions (O2 •–) and hydroxyl radicals (•OH) at higher concentrations (>500 μg/mL) but scavenge O2 •– at lower concentrations (40 μg/mL) with successful crossover at 200 μg/mL. Nanorods of MgO generate the highest levels of O2 •–, whereas nanoparticles scavenge O2 •– to the highest extent (60%). Photoluminescence studies reveal that such crossover is based on the suppression of F2+ and the evolution of F+, F2 +, and F2 3+ defect centers. The evolution of these defect centers reflects the antibacterial activity of MgO nanostructures which is initiated at 200 μg/mL against Gram-positive S. aureus ATCC 29737 and among different bacterial strains including Gram-positive B. subtilis ATCC 6633 and M. luteus ATCC 10240 and Gram-negative E. coli ATCC K88 and K. pneumoniae ATCC 10031. Nanoparticles exhibited the highest antibacterial (92%) and antibiofilm activity (17%) against B. subtilis ATCC 6633 in the dark. Interestingly, the nitrogen-centered free radical DPPH is scavenged (100%) by nanoplates due to its large surface area (342.2 m2/g) and the presence of the F2 + defect state. The concentration-dependent interaction with an antioxidant defense system (ascorbic acid (AA)) highlights nanoparticles as potent scavengers of O2 •– in the dark. Thus, our findings establish guidelines for the selection of MgO nanostructures for diverse therapeutic applications.

show abstract

Section: Results and Discussionsupporting

confidence: 83%

Section: Mgo Can Impart Oxidative Stress Via Production Of Omentioning

confidence: 99%

Effect of Morphology and Concentration on Crossover between Antioxidant and Pro-oxidant Activity of MgO Nanostructures

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Metal nanoparticles were widely reported to exhibit antibacterial effect 27–29 and anti-biofilm effect 30–33 few studies have also explored efflux inhibitory activity of metal NPs 34–36 . Reports on the ability of metal NPs to cure plasmids from bacterial cells are very scarce.…”

Section: Discussionmentioning

confidence: 99%

Sub lethal levels of platinum nanoparticle cures plasmid and in combination with carbapenem, curtails carbapenem resistant Escherichia coli

Bharathan

Sundaramoorthy

Chandrasekaran

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Drug resistance traits are rapidly disseminated across bacteria by horizontal gene transfer, especially through plasmids. Plasmid curing agents that are active both in vitro and in vivo will resensitize Multi Drug Resistant (MDR) bacteria to antimicrobial agents. Pectin capped platinum nanoparticles (PtNPs) at sub MIC (20 µM) concentration was effective, in causing loss of Extended Spectrum Beta Lactamase (ESBL) harboring plasmid as evidenced by, absence of plasmid in agarose gel and by a concomitant (16–64 fold) drop in MIC for cell wall inhibitors ceftriaxone and meropenem, in carbapenem resistant Escherichia coli (CREC). Interestingly, the plasmid cured strain exhibited small colony morphology and displayed slower growth both in vitro and in vivo . Complementation of cured strain with plasmid from the wild type strain restored resistance towards meropenem and ceftriaxone. Relative to wild type, plasmid cured strain displayed 50% reduction in biofilm formation. Plasmid curing also occurred in vivo in infected zebrafish with curing efficiency of 17% for nanoparticle + meropenem treatment. PtNPs + meropenem reduced bioburden of CREC in infected zebrafish by 2.4 log CFU. Mechanistic studies revealed that nanoparticle interacted with cell surface and perturbed inner membrane integrity. PtNPs did not induce ROS, yet it caused plasmid DNA cleavage, as evidenced by gyrase inhibition assay. Our study for the first time reveals that PtNPs as plasmid curing agent can resensitize MDR bacteria to selective antimicrobial agents in vivo .

show abstract

“…84 In the past few years, metal-based nanoparticles particularly silver and gold nanoparticle sensor systems have been largely investigated for the colorimetric sensing of metal ions such as Ni 2+ , Co 2+ , Hg 2+ , Pb 2+ , and Cu 2+ . 85 Since copper is much more cost-effective than silver or gold, N -lauryltyramine capped copper nanoparticles have been explored to selectively detect mercuric ions (Hg 2+ ) at micromolar concentrations in water bodies such as sewage. Additionally, Pd nanoparticles 105 have been known to play a key role in the development of ultrasensitive biosensors that help in the detection of various health issues.…”

Section: Applicationsmentioning

confidence: 99%

Recent applications of nanoparticles in organic transformations

et al. 2022

View full text Add to dashboard Cite

show abstract

N-lauryltyramine capped copper nanoparticles exhibit a selective colorimetric response towards hazardous mercury(ii) ions and display true anti-biofilm and efflux pump inhibitory effects in E. coli

Abstract: Hg2+ ions selectively decolorize CuNPs through aggregation.

Cited by 20 publications

References 66 publications

Effect of Morphology and Concentration on Crossover between Antioxidant and Pro-oxidant Activity of MgO Nanostructures

Effect of Morphology and Concentration on Crossover between Antioxidant and Pro-oxidant Activity of MgO Nanostructures

Sub lethal levels of platinum nanoparticle cures plasmid and in combination with carbapenem, curtails carbapenem resistant Escherichia coli

Recent applications of nanoparticles in organic transformations

Contact Info

Product

Resources

About