N-succinyl chitosan–dialdehyde starch hybrid hydrogels for biomedical applications

Abstract

A new class of injectable, biocompatible and biodegradable hydrogel is reported. This hydrogel is derived from N-succinyl chitosan (SCS) mixed with water-soluble dialdehyde starch (DAS) without using a conventional chemical crosslinker. The hybrid hydrogel is formed owing to the Schiff’s base reaction between amine groups of SCS and dialdehyde groups of DAS to form —CHN— group. SCS, DAS, and SCS–DAS hybrid hydrogels were synthesized and then characterized by FTIR analysis spectroscopy. The influence of SCS:DAS ratio in hybrid polymers solution on physicochemical properties of resultant hydrogels (e.g. gelation time, gel fraction (%) and equilibrium swelling ratio), surface morphology, in vitro weight loss (%), and mechanical stability was examined. The results demonstrated that SCS content has a profound role for forming tighter crosslinked hybrid hydrogels, where the increase of SCS content reduces the time for hydrogel forming. Also, the water uptake and hydrolytic weight loss decrease. Meanwhile, the DAS content increases, and mechanical properties of SCS–DAS hybrid hydrogels decrease. Curcumin release profile and adhered HGF cells on hydrogel surface sharply influenced the SCS portion in hybrid hydrogel composition. The SCS–DAS hybrid hydrogel properties afforded a possible opportunity to be used as a covalent in situ forming hybrid hydrogels in biomedical applications such as, tissue engineering and cartilage repair.

Advancements and Frontiers in the High Performance of Natural Hydrogels for Cartilage Tissue Engineering

“…To overcome the flaw of water insolubility, N-succinyl chitosan-dialdehyde starch mixture hydrogel was prepared with good solubility to repair cartilage defects (Kamoun, 2016). In addition, the sensitization and mechanism of chitosan may also need improvement in its clinical transformation.…”

Antimicrobial Chitosan Conjugates: Current Synthetic Strategies and Potential Applications

“…Although the presence of hydroxyl and amino groups in the chitosan molecule can allow structural modification to be easily carried out, in terms of conjugates that result in chitosan conjugates with more structural diversity, it is very necessary to explore a functional group conversion strategy to introduce new reactive groups to the chitosan backbone. It has been demonstrated that the amino group of chitosan can be converted into azide group [87], substituted carboxyl group [88], substituted mercapto group [89], etc., and the hydroxyl group can be azidated [90], aminated [91,92], oxidized to an aldehyde [93] or carbonyl group [94], or further oxidized to a carboxyl group [95]. Figure 4 lists some common functional group conversion methods used in the preparation of common chitosan conjugates.…”

Mesenchymal Stem Cell Culture on Composite Hydrogels of Hydroxyapatite Nanoparticles and Photo-Crosslinking Chitosan

“…Para la solubilización y modificación química del quitosano se utilizó el protocolo propuesto por Ono. K. y colaboradores [10] ; en el cual se funcionaliza el quitosano por medio de reacciones de carbodiimida con el ácido lactobionico y ácido azidobenzoico para aumen-tar la solubilidad del quitosano a pH fisiológico y producir un hidrogel por fotoentrecruzamiento, respectivamente. Para este proceso se utilizó quitosano con un peso molecular promedio de 4.3x10 5 Da.…”

“…La fase de adhesión celular se lleva a cabo en períodos más largos e involucra moléculas de adhesión y del citoesqueleto (como filamentos de actina), los cuales van a interactuar para producir una respuesta bien sea en proliferación o diferenciación celular [24] . química del producto final [10,[25][26] .…”

“…Además de esto, generalmente, se utiliza un agente entrecruzante que puede conllevar ciertos niveles de citotoxicidad, actuando en detrimento del aspecto biocompatible del biopolímero. Como consecuencia, algunos investigadores han propuesto el uso de otros agentes entrecruzantes, y modificaciones que permitan la disolución del QT a pH fisiológico [9,10] .…”