N-Terminal Deletion Effects of Human Survivin on Dimerization and Binding to Smac/DIABLO in Vitro

Abstract

Survivin, as an apoptosis suppressor, exists as a homodimer interfacing at the N-terminal portion (residues 6-13) of its baculovirus IAP repeat (BIR) domain and a linker segment (residues 89-102). Here we expressed full-length human Survivin (SurF) and a series of its mutants, SurΔN7, SurΔN13, and SurΔN18 with significant truncations of the N-terminus, all of which could still dimerize in solution. Single-molecule force spectroscopy (SMFS) was used to quantitate the unbinding forces of full-length and the mutant homodimers and revealed that the N-terminal residues up to Arg18 were not essential for dimerization. Meanwhile, the binding of SurΔN7 to Smac/DIABLO determined by ELISA was as efficient as the wild-type, but that of SurΔN13 was significantly reduced, and that of SurΔN18 was completely lost. Together, these findings provide direct evidence that the N-terminal sequence of Survivin is not critical for dimer formation but may contribute to correct folding and function of BIR.

The switch-on luminescence sensing of histidine-rich proteins in solution: a further application of a Cu2+ ligand

“…Chem., 2011, 9, 4032-4034 | 4033 (20 mM) the non-His-tag GST showed no fluorescence recovery. Simultaneously, the His-tagged survivin 14 was also used to clarify the switch-on fluorescence response of 1/Cu 2+ to the histidine-rich protein, which show very similar result as the His-tagged GST (Fig. 5).…”

Survivin inhibition by an interacting recombinant peptide, derived from the human ferritin heavy chain, impedes tumor cell growth

“…The survivin monomer associates with borealin and inner centromere proteins to regulate chromosome segregation and cytokinesis, and the homodimer, dependent upon hydrophobic contacts at the interaction surface, has anti-apoptotic functions (Gao et al 2010). We also investigated the presumed dimerization of survivin in the yeast two-hybrid system.…”

Palladium nanoparticles supported on poly (N-vinylpyrrolidone)-grafted silica as new recyclable catalyst for Heck cross-coupling reactions

“…This technique has provided an opportunity to take the advantage of a solid linear functional polymer that can be completely compatible with solvents and substrates and at the same time having high mechanical stability and good dispersion [24]. Chemical bindings of polymer chains onto silica have been studied with various monomers, such as; styrene [25], vinyl acetate [26], acrylic acid [23], methyl acrylate [27], vinylpyrrolidone [20,24], vinylimidazole [28] and vinylpyridine [29]. In 2008 Gao et al reported the preparation of poly (4-vinylpyridine)eCu complex grafted silica and its use as a heterogeneous catalyst in oxidation reactions [29].…”

“…Chemical bindings of polymer chains onto silica have been studied with various monomers, such as; styrene [25], vinyl acetate [26], acrylic acid [23], methyl acrylate [27], vinylpyrrolidone [20,24], vinylimidazole [28] and vinylpyridine [29]. In 2008 Gao et al reported the preparation of poly (4-vinylpyridine)eCu complex grafted silica and its use as a heterogeneous catalyst in oxidation reactions [29]. In 2009 Han et al used poly (N-vinylpyrrolidone)eRu complex grafted silica as a catalyst for hydrogenation of aromatic compounds [30].…”