N-terminal HrpE from Xanthomonas oryzae pv. oryzae mediates the regulation of growth and photosynthesis in rice

Essential Acidovorax citrulli Virulence Gene hrpE Activates Host Immune Response against Pathogen

“…We mainly ascribe the present results to the interaction targets of HrpE in hosts, which might bring HrpE into the nucleus. This localization may also be related to its functions of enhancing photosynthesis and promoting plant growth [ 30 , 31 , 32 ]. The specific mechanism of this phenomenon needs to be further studied and may further elucidate the role of HrpE in pathogen-host interaction.…”

“…HrpE was found to be recognized by host cells and induce host immune response and could therefore help resist the invasion of pathogenic bacteria to some extent [ 30 , 31 ]. HrpE even enhanced leaf photosynthesis efficiency in the host [ 31 , 32 ]. Based on the results above, we speculate that HrpE may serve as a PAMP, further, a harpin that can be recognized by host plants and induce host PTI.…”

Volatile Organic Compounds Emitted by the Biocontrol Agent Pythium oligandrum Contribute to Ginger Plant Growth and Disease Resistance

“…For the extraction of the hormones, 0.1 g of fresh (not dried) leaf blades from each treatment was ground to a fine powder using a TissueLyser (60 s at 50 Hz). The fine leaf powder was then used to prepare the samples for hormone quantification based on a method described previously ( 93 ). Briefly, the powder was extracted with 1 mL of 80% methanol in a 2-mL tube by shaking at 300 rpm overnight at 4°C.…”

“…The supernatant from the samples was transferred to a new tube and then dried by vacuum evaporation at room temperature for 6 h, and then 200 μL of 80% methanol was added to the dried samples to dissolve the extract and then passed through a 0.2-μm-pore-size filter. The standard chemicals were purchased from Macklin, China for each of the quantified hormones (IAA, GA3, ZEA, and ABA) and standard solutions of different concentrations (0.1, 0.2, 0.5, 2, 5, 20, 50, and 200 ng/mL) were prepared in 80% methanol as described previously ( 93 ), and samples were run on a Xevo TQ-S (Waters, USA) machine coupled with the Waters Acquity HSS T3 ultraperformance (UPLC) column (1.8 μm, 2.1 mm by 75 mm) with an injection volume of 2 μL at a column temperature of 30°C for detection and quantification ( 94 ).…”