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Colville, Anne; Lim, Richard P.

doi:10.1071/mr02014

Cited by 20 publications

(2 citation statements)

References 45 publications

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“…In this study, the biochemical and ultrastructural features of melanin in mantle of Pacific oyster was systematically described. From a morphological perspective, the organization structure of the mantle edge is very similar in the Velesunio ambiguous (Colville & LimA, 2003) and…”

Section: Discussionmentioning

confidence: 99%

“…Haliotis asinine (McDougall et al, 2011), which was thickened and formed into three major folds: outer fold that are required for secretion, and middle fold that plays a role in sensation and inner fold acting in muscle. Because the periostracum emerged from the groove between the outer and middle folds, the out fold was further divided into two or more lobes (Colville & LimA, 2003). However, with minor differences in the appearance of the number of middle grooves in C. gigas and other species.…”

Section: Discussionmentioning

confidence: 99%

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Histological, elemental, and ultrastructural analysis of melanin in mantle of Pacific oyster (Crassostrea gigas)

et al. 2022

Microscopy Res & Technique

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Colorful shell of bivalve is mainly because of the biological pigments, of which melanin plays an important role in shell color formation. More and more studies focus on the genes function involved in melanin synthesis, but relatively few studies address the biochemical character and ultrastructure of melanin in bivalve from microscopic perspective. Here, we investigated the histological structure of mantle of Crassostrea gigas with orange shell color. Distribution of melanin in mantle was verified with histochemical staining. In addition, immunofluorescence technique showed that strongly positive signal of CgTYR was specific to the mantle margin, which is consistence with the location of brown granules in H&E staining. The further result of elementary composition of melanin displayed that metal Ca, Fe, and Zn were detected using scanning transmission electron microscope and energy dispersive spectroscopy mapping methods. Next, based on TEM observations, it was speculated that the series of cellular events leading to the formation and release of melanin. Melanocyte in the primary stage showed many mitochondria and rough endoplasmic reticulum as well as an extensive Golgi complex with numerous vesicles intermingled with melanosome.Subsequently, melanosome was expended and their hue gradually intensified, and Golgi complex and mitochondria were still observed in the cytoplasm. Finally, after melanosome was discharged into intercellular spaces, the disintegration of membranes in some cells, and severe cellular vacuolization. These data enrich the understanding of ultrastructural characteristic and formation of melanin in mantle of bivalve and pave the way for further investigating shell coloration at the cellular level.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Histological, elemental, and ultrastructural analysis of melanin in mantle of Pacific oyster (Crassostrea gigas)

et al. 2022

Microscopy Res & Technique

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Spermatogenic Cycle and Steroidogenic Control of Spermatogenesis in Mytilus galloprovincialis Collected in the Bay of Naples

Prisco¹,

Agnese²,

Marino³

et al. 2017

The Anatomical Record

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The aim of the present article was to study the spermatogenic cycle of Mytilus galloprovincialis collected in the Bay of Naples during a whole year and to acquire new insights into the mechanism of control. Knowledge of the Mytilus cycle in this geographic area is of particular interest as, to the best of our knowledge, the male gonad cycle has been hitherto unexplored. Testis organization was evaluated together with the localization of the enzymes 3β-HSD, 17β-HSD, and P450-aromatase, which are strictly connected to the synthesis of two key hormones involved in the testis activity: testosterone and 17β-estradiol. It was demonstrated that: (1) the spermatogenic cycle starts in late Summer-early Fall and continues until early Winter, when the first spawning occurs; after rapid gonad restoration, several spawning events take place until June, when the testis becomes non-active again; (2) in the testis, true Leydig and Sertoli cells are present; (3) during the reproductive period, Sertoli, Leydig, germ, and adipogranular cells (ADGs) are positive to 3β-HSD and 17β-HSD, while only germ cells are positive to P450 aromatase; by contrast, during the resting period, only ADGs are positive to 3β-HSD and 17β-HSD, and P450-aromatase is no longer recognizable. The presence of a hermaphrodite sample is also described. Anat Rec, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 300:1881-1894, 2017. © 2017 Wiley Periodicals, Inc.

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Application of analytical electron microscopic methods to investigate the function of spherites in the midgut of the larval antlion Euroleon nostras (Neuroptera: Myrmeleontidae)

Lipovšek

Letofsky-Papst

Hofer

et al. 2011

Microscopy Res & Technique

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This study presents an application of analytical electron microscopy in biology to investigate the chemical composition of the spherites and to elucidate the importance of these methods in the life sciences. The structure of the spherites in the midgut cells of first, second, and third instar larvae Euroleon nostras was investigated by a combination of transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS), and energy filtering TEM (EFTEM). The structure and chemical composition of the spherites changed during the metamorphosis. In first larvae, the spherites are composed of amorphous, flocculent material, containing C, N, and O. In second larvae and third ones, the spherites have concentric layers of alternating electron-dense and electron-lucent material. In second larvae, Si, P, Ca, and Fe are accumulated in the spherite organic matrix, composed of C, N, and O. In the spherites of third larvae, additionally Al was found. Therefore, the spherites are thought to store organic compounds in all three larval stages of E. nostras and additionally inorganic compounds in second and third ones. In first larvae, spherites are present in the midgut cells; in second and third larvae, they are present in the cells of the midgut and in its lumen. It could be suggested that the spherites might be involved in the regulation of the appropriate mineral composition of the internal environment and could serve as the accumulation site of nontoxic waste materials that cannot be metabolized.

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Cited by 20 publications

References 45 publications

Histological, elemental, and ultrastructural analysis of melanin in mantle of Pacific oyster (Crassostrea gigas)

Histological, elemental, and ultrastructural analysis of melanin in mantle of Pacific oyster (Crassostrea gigas)

Spermatogenic Cycle and Steroidogenic Control of Spermatogenesis in Mytilus galloprovincialis Collected in the Bay of Naples

Application of analytical electron microscopic methods to investigate the function of spherites in the midgut of the larval antlion Euroleon nostras (Neuroptera: Myrmeleontidae)

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