Na,K-ATPase is essential for embryonic heart development in the zebrafish

Shu, Xiaodong; Cheng, Karen Y.; Patel, Neil; Chen, Fuhua; Joseph, Elaine M.; Tsai, Huai‐Jen; Chen, Jau-Nian

doi:10.1242/dev.00844

Cited by 107 publications

(99 citation statements)

References 33 publications

(41 reference statements)

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“…The morphological m883 phenotype correlates with those published for other atp1a1a alleles: severe heart defects have been shown previously to entail a smaller heart, thin ventricular wall, and disrupted chamber specificity of cardiomyocytes, which together cause a slow heart beat and weak contractility (Shu et al, 2003). atp1a1a.1 has also been shown to be required for the inflation of the brain ventricle lumen (Lowery and Sive, 2005) Expression of atp1a1a.1…”

Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal supporting

confidence: 73%

“…Molecular identification of the Na, K-ATPase subunit atp1a1a.1 as the gene affected in m883 for the first time provides genetic evidence for the previously postulated role for an ion pump during establishment of laterality in general. Previously, four other zebrafish alleles of atp1a1a.1 were published (Shu et al, 2003;Amsterdam et al, 2004;Yuan and Joseph, 2004;Lowery and Sive, 2005). While the alleles published by Shu and coworkers (heart and mind, had) and Yuan and coworkers (small heart) appear to be identical, the insertional mutation published by Amsterdam and coworkers has not been described in detail.…”

Section: Discussionmentioning

confidence: 99%

“…Fine mapping with a single embryo panel representing 246 meioses revealed no recombination events between m883 and the markers z12036 and z6384 at 7,4 cM on chromosome 1. A search for candidate genes in this region (www.ZFIN.org) revealed that Chen and colleagues had mapped the mutation heart and mind (had la1 ) to this genomic region and shown that had affects the gene ATPase, Naϩ/Kϩ transporting, alpha 1a.1 polypeptide (atp1a1a.1) (Shu et al, 2003). More recently, other alleles of had have also been published: had hi3475 , a retroviral insertion (Amsterdam et al, 2004), had mw15b (Yuan and Joseph, 2004), and had to273a (Lowery and Sive, 2005), which initially had been described as snk to273a .…”

Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal mentioning

confidence: 99%

“…In collaboration with Dr. Chen (UCLA), we performed complementation analysis of m883 and had la1 , which revealed non-complementation and demonstrated that m883 is an allele of had. The atp1a1a.1 locus has previously also been called Na,K-ATPase alpha1B1 (Shu et al, 2003); however, the nomenclature has been changed (Rajarao et al, 2001) and www.zfin.org).…”

Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal mentioning

confidence: 99%

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A mutation in the zebrafish Na,K‐ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left‐right asymmetry downstream or in parallel to nodal flow

Ellertsdóttir

Ganz

Dürr

et al. 2006

Developmental Dynamics

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While there is a good conceptual framework of dorsoventral and anterioposterior axes formation in most vertebrate groups, understanding of left-right axis initiation is fragmentary. Diverse mechanisms have been implied to contribute to the earliest steps of left-right asymmetry, including small molecule signals, gap junctional communication, membrane potential, and directional flow of extracellular liquid generated by monocilia in the node region. Here we demonstrate that a mutation in the zebrafish Na,K-ATPase subunit atp1a1a causes left-right defects including isomerism of internal organs at the anatomical level. The normally left-sided Nodal signal spaw as well as its inhibitor lefty are expressed bilaterally, while pitx2 may appear random or bilateral. Monocilia movement and fluid circulation in Kupffer's vesicle are normal in atp1a1a m883 mutant embryos. Therefore, the Na,K-ATPase is required downstream or in parallel to monocilia function during initiation of left-right asymmetry in zebrafish.

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Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal supporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal mentioning

confidence: 99%

Section: The M883 Mutation Causes Left-right Isomerism Of Endodermal mentioning

confidence: 99%

See 2 more Smart Citations

A mutation in the zebrafish Na,K‐ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left‐right asymmetry downstream or in parallel to nodal flow

Ellertsdóttir

Ganz

Dürr

et al. 2006

Developmental Dynamics

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show abstract

“…Mutations in the genes heart and soul (prcki), snakehead (atp1a1a.1), and nagie oko (mpp5) hinder heart tube extension: mutant hearts resemble arrested cardiac cones or stunted heart tubes [12,[30][31][32][33][34]. The PRKCi, Atp1a1a.…”

Section: Defining Cardiac Cytoarchitecture: Subcellular Mechanics Of mentioning

confidence: 99%

Illuminating cardiac development: Advances in imaging add new dimensions to the utility of zebrafish genetics

Schoenebeck

Yelon

2007

Seminars in Cell & Developmental Biology

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The use of the zebrafish as a model organism for the analysis of cardiac development is no longer proof-of-principle science. Over the last decade, the identification of a variety of zebrafish mutations and the subsequent cloning of mutated genes have revealed many critical regulators of cardiogenesis. More recently, increasingly sophisticated techniques for phenotypic characterization have facilitated analysis of the specific mechanisms by which key genes drive cardiac specification, morphogenesis, and function. Future enrichment of the arsenal of experimental strategies available for zebrafish should continue the yield of high returns from such a small source.

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Is the undergraduate research experience (URE) always best?: The power of choice in a bifurcated practical stream for a large introductory biochemistry class

Rowland

Lawrie

Behrendorff

et al. 2012

Biochem Molecular Bio Educ

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Science undergraduate courses typically cater to a mixed-learner cohort, with a diversity of motivations and skills. This diversity introduces pressure for designers of the practical laboratory curriculum. Students who are struggling with the course need a series of tasks that begin simply, and transition to more conceptually difficult material. More capable students need opportunities for conceptual extension and creative activity. In this report, we examine an approach we have used to address this problem in the context of a large introductory biochemistry undergraduate class. Rather than attempting to compromise on a single practical series for our 470 students, we devised two parallel but equivalent practical streams and offered students their choice of laboratory experience. One stream (called Laboratory Experience for Acquiring Practical Skills) was designed to allow acquisition of a range of common biochemistry and molecular biology laboratory skills. The other (called Active Learning Laboratory Undergraduate Research Experience) was designed to offer an authentic (but scaffolded) undergraduate research project. We discuss the ramifications and implications of our approach in terms of funding, staffing, and assessment while also examining student motivation, satisfaction, and skills acquisition. We present data supporting the practical and pedagogical value of laboratory exercise streaming to meet the diverse needs of students. We suggest a framework that can be used to pre-emptively identify and address problems associated with a bifurcated practical series and increase the sustainability of the approach.Keywords: Active learning, assessment of educational activities, curriculum, design development and implementation, laboratory exercises, learning and curriculum design, new course development.The practical laboratory is central to an undergraduate science education [1]. The types of learning that occur in an undergraduate laboratory can (and do) range from the simple improvement of practical skills, to the development of an abstract understanding of the nature of science. There is evidence that the learning gains achieved by students are affected by the design of the laboratory exercise [2], and this idea (in theory) is well accepted in the literature. According to the dogma, a traditional ''cookbook'' laboratory exercise, for example, is likely to produce only gains at the lower points of the cognitive scale as defined in Bloom's Revised Taxonomy [3]. In contrast, a research-driven laboratory learning experience has potential to produce students with an increased ability to think in an abstract manner, define and investigate their own research problems, and develop an understanding of what it means to ''be a scientist''.There have been widespread recommendations that undergraduate science teaching should adopt researchbased learning as standard [4]. This has led to a wave of implementation and debate around student-centered, inquiry-based, and research-based laboratory programs [5][6][7]. Contexts for ...

show abstract

Na,K-ATPase is essential for embryonic heart development in the zebrafish

Cited by 107 publications

References 33 publications

A mutation in the zebrafish Na,K‐ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left‐right asymmetry downstream or in parallel to nodal flow

A mutation in the zebrafish Na,K‐ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left‐right asymmetry downstream or in parallel to nodal flow

Illuminating cardiac development: Advances in imaging add new dimensions to the utility of zebrafish genetics

Is the undergraduate research experience (URE) always best?: The power of choice in a bifurcated practical stream for a large introductory biochemistry class

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