NADPH‐diaphorase distribution in the rabbit superior colliculus and co‐localization with calcium‐binding proteins

González‐Soriano, Juncal; Contreras‐Rodríguez, Julio; Martı́nez-Sainz, P; Martín‐Palacios, Susana; Marı́n-Garcı́a, P.; Rodríguez‐Veiga, Elisia

doi:10.1046/j.1469-7580.2002.00025.x

Cited by 12 publications

(5 citation statements)

References 43 publications

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“…In the superficial layers of the rat SC, all different cell types described with de Golgi technique (Langer and Lund, 1974) were shown to express nNOS (González-Hernández et al, 1992;Tenório et al, 1996;Soares-Mota et al, 2001), and the presence of the nNOS in these layers has also been reported in different mammalian species (González-Hernández et al, 1993;Egberongbe et al, 1994;Satoh et al, 1995;Tenório et al, 1995Tenório et al, , 1996Giraldi-Guimarães et al, 1999;Cork et al, 2000;Scheiner et al, 2001;González-Soriano et al, 2002). During development of the rat SC the first cells expressing nNOS were detected by the end of the first postnatal week.…”

Section: Introductionmentioning

confidence: 83%

A quantitative study of the neuronal nitric oxide synthase expression in the superficial layers of the adult rat superior colliculus after perinatal enucleation

Bittencourt-Navarrete

Giraldi-Guimarães

Mendez-Otero

2004

Intl J of Devlp Neuroscience

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The level of expression of the neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of rat superior colliculus (SC) was investigated in adult rats after neonatal enucleation using two biochemical methods: (1) measurement of the in vitro specific-activity of NOS by the conversion of [3H]-arginine to [3H]-citrulline and (2) immunochemical analysis by western blotting and densitometry of immunoreactive bands using antibodies that recognise the three prominent isoforms of nNOS, alpha, beta and gamma. A total of 20 Lister rats were used in this study. We have shown that the deprivation of the retinocollicular projections at early postnatal ages induces no significant change in the specific-activity of nNOS. We also have shown that the deafferentation does not significantly influence either the total amount of nNOS in the SC superficial layers or the relative contribution ratio of nNOS isoforms. In conclusion, the expression and activity of nNOS in the SC retinorecipient layers was shown not to be dependent on the presence of retinal afferents during development.

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Section: Introductionmentioning

confidence: 83%

A quantitative study of the neuronal nitric oxide synthase expression in the superficial layers of the adult rat superior colliculus after perinatal enucleation

Bittencourt-Navarrete

Giraldi-Guimarães

Mendez-Otero

2004

Intl J of Devlp Neuroscience

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“…Nitric oxide (NO) was recognized as the first gaseous neurotransmitter with a very short half-life time (2–6 s) (Greenwood and Earnshaw, 1997 ; Barañano et al, 2001 ; González-Soriano et al, 2002 ) and it has been implicated as a non-adrenergic non-cholinergic (NANC) inhibitory neurotransmitter at various sites in the nervous system (Grozdanovic et al, 1994 ; Schuman and Madison, 1994 ; Sharma et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…The activation occurs via interaction of NO with Fe +2 in the heme portion of the molecule, thereby altering its conformation and activating it, this causes conversion of guanosine triphosphate (GTP) to cyclic guanosine monophosphate (cGMP), which mediates many of the physiological actions of NO in mammalian cells (McCann et al, 2005 ). In aldehyde fixed tissue, nitric oxide synthase (NOS) is able to selectively catalyze a histochemically detectable Golgi-like dense staining of reactive neurons produced by Nicotinamide Adinine Dinulceotide Phosphate diaphorase (NADPH-d) histochemistry (González-Soriano et al, 2002 ). This is known as NADPH-diaphorase activity, which is used as a histochemical detection method for neuronal NO-producing structures (Bredt et al, 1991 ).…”

Section: Introductionmentioning

confidence: 99%

Distribution of nitric oxide-producing cells along spinal cord in urodeles

Mahmoud

Fahmy

Moftah

et al. 2014

Front. Cell. Neurosci.

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Nitric oxide is a unique neurotransmitter, which participates in many physiological and pathological processes in the organism. There are little data about the neuronal nitric oxide synthase immunoreactivity in the spinal cord of amphibians. In this respect, the present study aims to investigate the distribution of nitric oxide producing cells in the spinal cord of urodele and to find out the possibility of a functional locomotory role to this neurotransmitter. The results of the present study demonstrate a specific pattern of NADPH-d labeling in the selected amphibian model throughout the spinal cord length as NADPH-d-producing cells and fibers were present in almost all segments of the spinal cord of the salamander investigated. However, their number, cytological characteristics and labeling intensity varied significantly. It was noticed that the NO-producing cells (NO-PC) were accumulated in the ventral side of certain segments in the spinal cord corresponding to the brachial and sacral plexuses. In addition, the number of NO-PC was found to be increased also at the beginning of the tail and this could be due to the fact that salamanders are tetrapods having bimodal locomotion, namely swimming and walking.

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“…The presence of NOS-positive cells in the retinorecipient superficial layers of the superior colliculus has been reported in several mammalian species (19)(20)(21)(22)(23)(24)(25). In the rat, a subpopulation of all the different cell types described in the superficial layers of the superior colliculus by the Golgi technique (26) expresses nNOS (21,27,28).…”

Section: Introductionmentioning

confidence: 99%

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

Giraldi-Guimarães

Bittencourt-Navarrete

Mendez-Otero

2004

Braz J Med Biol Res

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We investigated the level of expression of neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of the rat superior colliculus during early postnatal development. Male and female Lister rats ranging in age between the day of birth (P0) and the fourth postnatal week were used in the present study. Two biochemical methods were used, i.e., in vitro measurement of NOS specific activity by the conversion of [ 3 H]-arginine to [ 3 H]-citrulline, and analysis of Western blotting immunoreactive bands from superior colliculus homogenates. As revealed by Western blotting, very weak immunoreactive bands were observed as early as P0-2, and their intensity increased progressively at least until P21. The analysis of specific activity of NOS showed similar results. There was a progressive increase in enzymatic activity until near the end of the second postnatal week, and a nonsignificant tendency to an increase until the end of the third week was also observed. Thus, these results indicated an increase in the amount of nNOS during the first weeks after birth. Our results confirm and extend previous reports using histochemistry for NADPH-diaphorase and immunocytochemistry for nNOS, which showed a progressive increase in the number of stained cells in the superficial layers during the first two postnatal weeks, reaching an adult pattern at the end of the third week. Furthermore, our results suggested that nNOS is present in an active form in the rat superior colliculus during the period of refinement of the retinocollicular pathway.

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NADPH‐diaphorase distribution in the rabbit superior colliculus and co‐localization with calcium‐binding proteins

Cited by 12 publications

References 43 publications

A quantitative study of the neuronal nitric oxide synthase expression in the superficial layers of the adult rat superior colliculus after perinatal enucleation

A quantitative study of the neuronal nitric oxide synthase expression in the superficial layers of the adult rat superior colliculus after perinatal enucleation

Distribution of nitric oxide-producing cells along spinal cord in urodeles

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

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