NADPH oxidase, MPO, NE, ERK1/2, p38 MAPK and Ca2+ influx are essential for Cryptosporidium parvum-induced NET formation

Muñoz-Caro, Tamara; Lendner, Matthias; Daugschies, Arwid; Hermosilla, Carlos; Taubert, Anja

doi:10.1016/j.dci.2015.05.007

Cited by 93 publications

(97 citation statements)

References 60 publications

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“…Interestingly, it has been recently demonstrated that PMN, which undergo NETosis without cell lysis, are still live and retain their capability of phagocytosis (8). Consistent with these reports, PMN also seem to be capable of extruding NETs of mitochondrial origin, which are of smaller size than those originating from classical induced NETosis by different forms of NETs, such as aggregated (aggNETs), diffused (diffNETs), and spread (sprNETs) NETs, that have been recently reported (9,10).…”

supporting

confidence: 68%

“…Zymosan 1 mg/mL (Invitrogen) was used to induce the formation of NETs in human PMN (positive control) as described elsewhere (10). The effect of different inhibitors of PMN/sperm samples ratio 1:6 (25 Â 10 4 :15 Â 10 5 ) was also assessed.…”

Section: Nets Quantificationmentioning

confidence: 99%

“…Quantification of NETs formation was determined by spectrofluorometric analysis at an excitation wavelength of 484 nm and an emission wavelength of 520 nm using an automated plate monochrome reader (Varioskan Flash; Thermo Scientific), as described elsewhere (6,10,13).…”

Section: Nets Quantificationmentioning

confidence: 99%

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Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Zambrano

Carrau

Gärtner

et al. 2016

Fertility and Sterility

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supporting

confidence: 68%

Section: Nets Quantificationmentioning

confidence: 99%

See 1 more Smart Citation

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Zambrano

Carrau

Gärtner

et al. 2016

Fertility and Sterility

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“…The results showed that N. caninum tachyzoite-induced NETs formation was significantly decreased by the SOCE inhibitor 2-APB. There is another report showing that SOCE is regulated via ERK 1/2 phosphorylation and via a ROS production-dependent activation of the Raf–MEK–ERK pathway, which has also been proved to be required for NETs formation induced by parasites (23, 27, 28). Then, we analyzed the critical role of Ca 2+ influx and the role of ERK 1/2 and p38 signaling pathway in N. caninum tachyzoite-induced NETs.…”

Section: Discussionmentioning

confidence: 99%

Canine Neutrophil Extracellular Traps Release Induced by the Apicomplexan Parasite Neospora caninum In Vitro

et al. 2016

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Neosporosis is considered as one of the main causes of abortion and severe economic losses in dairy industry. The Canis genus serving as one of the confirmed definitive hosts of the apicomplexan parasite Neospora caninum (N. caninum) plays a critical role in its life cycle. However, the effects of N. caninum on its definitive hosts of neutrophils extracellular traps (NETs) formation remain unclear. In the present study, N. caninum tachyzoite-induced canine NETs formation was observed by scanning electron microscopy (SEM). Visualization of DNA decorated with H3, neutrophil elastase (NE), and myeloperoxidase (MPO) within N. caninum tachyzoite-induced NETs were examined using fluorescence confocal microscopy analyses. Furthermore, the formation of canine NETs was quantified using Sytox Green staining, and the LDH levels in supernatants were examined by an LDH Cytotoxicity Assay® kit. The results clearly showed that NETs-like structures were induced by N. caninum tachyzoites, and the major components within these structures induced by N. caninum tachyzoite were further confirmed by fluorescence confocal microscopy visualization. These results suggest that N. caninum tachyzoites strongly induced NETs formation in canine polymorphonuclear neutrophils (PMN). In functional inhibition assays, the blockings of NADPH oxidase, NE, MPO, SOCE, ERK 1/2, and p38 MAPK signaling pathways significantly inhibited N. caninum tachyzoite-induced NETs formation. To our knowledge, this study is the first to report the formation of NETs in canine PMN against N. caninum infection.

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“…Neutrophil extracellular trap (NET) is an effective bactericidal mechanism whose first step depends on the ROS that are derived from NADPH oxidase activation [31,32] . Neutrophil cytosolic factor 1 (an essential component of the NOX 2 complex)-mutated mice lacked formation of NETs when they developed arthritis [33] .…”

Section: Inflammatory Reaction Stagementioning

confidence: 99%

Hydrogen Peroxide: A Potential Wound Therapeutic Target

Zhu¹,

Wang²,

Lu³

et al. 2017

Med Princ Pract

114

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Hydrogen peroxide (H2O2) is a topical antiseptic used in wound cleaning which kills pathogens through oxidation burst and local oxygen production. H2O2 has been reported to be a reactive biochemical molecule synthesized by various cells that influences biological behavior through multiple mechanisms: alterations of membrane potential, generation of new molecules, and changing intracellular redox balance, which results in activation or inactivation of different signaling transduction pathways. Contrary to the traditional viewpoint that H2O2 probably impairs tissue through its high oxidative property, a proper level of H2O2 is considered an important requirement for normal wound healing. Although the present clinical use of H2O2 is still limited to the elimination of microbial contamination and sometimes hemostasis, better understanding towards the sterilization ability and cell behavior regulatory function of H2O2 within wounds will enhance the potential to exogenously augment and manipulate healing.

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NADPH oxidase, MPO, NE, ERK1/2, p38 MAPK and Ca2+ influx are essential for Cryptosporidium parvum-induced NET formation

Cited by 93 publications

References 60 publications

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility

Canine Neutrophil Extracellular Traps Release Induced by the Apicomplexan Parasite Neospora caninum In Vitro

Hydrogen Peroxide: A Potential Wound Therapeutic Target

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