Nalidixic Acid-Resistant Strains of Salmonella Showing Decreased Susceptibility to Fluoroquinolones in the Midwestern Region of the Republic of Ireland Due to Mutations in the
            <i>gyrA</i>
            Gene

Ryan, Michael P.; Dillon, Colm; Adley, Catherine C.

doi:10.1128/jcm.02574-10

Cited by 19 publications

(9 citation statements)

References 13 publications

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“…However, the prevalence of broiler isolates resistant to CIP and NOR was higher here than in previous studies. The higher prevalence of resistance indicated a possible decrease in fluoroquinolone susceptibility, which may reflect an association between the high proportion of resistance to NAL (49.50%), CIP, and NOR in broiler isolates [40,41]. The NAL and fluoroquinolone-resistant Salmonella spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

Phongaran

Khang-Air

2019

Vet World

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Aim: This study aimed to determine the prevalence and antimicrobial resistance pattern of Salmonella spp., and the genetic relatedness between isolates from broilers and pigs at slaughterhouses in Thailand. Materials and Methods: Fecal samples (604 broilers and 562 pigs) were collected from slaughterhouses from April to July 2018. Salmonella spp. were isolated and identified according to the ISO 6579:2002. Salmonella-positive isolates were identified using serotyping and challenged with nine antimicrobial agents: Amoxicillin/clavulanate (AMC, 30 μg), ampicillin (AMP, 10 μg), ceftazidime (30 μg), chloramphenicol (30 μg), ciprofloxacin (CIP, 5 μg), nalidixic acid (NAL, 30 μg), norfloxacin (10 μg), trimethoprim/sulfamethoxazole (SXT, 25 μg), and tetracycline (TET, 30 μg). Isolates of the predominant serovar Salmonella Typhimurium were examined for genetic relatedness using pulsed-field gel electrophoresis (PFGE). Results: Salmonella was detected in 18.05% of broiler isolates and 37.54% of pig isolates. The most common serovars were Kentucky, Give, and Typhimurium in broilers and Rissen, Typhimurium, and Weltevreden in pigs. Among broilers, isolates were most commonly resistant to antibiotics, NAL, AMP, TET, AMC, and CIP. Pig isolates most commonly exhibited antimicrobial resistance against AMP, TET, and SXT. Based on PFGE results among 52 S. Typhimurium isolates from broilers and pigs, a high genetic relatedness between broiler and pig isolates (85% similarity) in Cluster A and C from PFGE result was identified. Conclusion: The results revealed high cross-contamination between these two animal species across various provinces in Thailand. Keywords: antimicrobial resistance, broilers, pigs, pulsed-field gel electrophoresis, Salmonella spp.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

Phongaran

Khang-Air

2019

Vet World

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“…Ciprofloxacin resistance was also predicted by this genotype, but the strain was designated as susceptible based on a MIC of 0.25 ng/μl. Quinolones such as nalidixic acid and ciprofloxacin are widely used first-line therapeutics for humans with salmonellosis when antimicrobial therapy is warranted ( 51 ), and increasing incidences of therapeutic failure in treating salmonellosis with ciprofloxacin have resulted in requests to re-evaluate human breakpoints for this drug ( 52 , 53 ). Nevertheless, nalidixic acid resistant SE strains like SE_TAU19 have emerged in the southeastern United States ( 54 ) and elsewhere ( 55 ) contributing to the public health threat of drug-resistant, foodborne SE.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of a Nalidixic Acid-Resistant Salmonella Enteritidis Strain Causing Persistent Infections in Broiler Chickens

et al. 2021

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Virulent strains of Salmonella enterica subsp. enterica serovar Enteritidis (SE) harbored by poultry can cause disease in poultry flocks and potentially result in human foodborne illness. Two broiler flocks grown a year apart on the same premises experienced mortality throughout the growing period due to septicemic disease caused by SE. Gross lesions predominantly consisted of polyserositis followed by yolk sacculitis, arthritis, osteomyelitis, and spondylitis. Tissues with lesions were cultured yielding 59 SE isolates. These were genotyped by Rep-PCR followed by whole-genome sequencing (WGS) of 15 isolates which were clonal. The strain, SE_TAU19, was further characterized for antimicrobial susceptibility and virulence in a broiler embryo lethality assay. SE_TAU19 was resistant to nalidixic acid and sulfadimethoxine and was virulent to embryos with 100% mortality of all challenged broiler embryos within 3.5 days. Screening the SE_TAU19 whole-genome sequence revealed seven antimicrobial resistance (AMR) genes, 120 virulence genes, and two IncF plasmid replicons corresponding to a single, serovar-specific pSEV virulence plasmid. The pef, spv, and rck virulence genes localized to the plasmid sequence assembly. We report phenotypic and genomic features of a virulent SE strain from persistently infected broiler flocks and present a workflow for SE characterization from isolate collection to genome assembly and sequence analysis. Further SE surveillance and investigation of SE virulence in broiler chickens is warranted.

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“…33 As far as the 1 H NMR measurements are concerned, the results in CD3CN listed in Table 1 show the chemical shifts of the free receptor, , and the chemical shift changes, , for the receptor upon the addition of the pharmaceutical. No chemical shifts changes were found by the addition of other drugs containing carboxylic functionalities such as ciprofloxacin (antibiotic used against a wide range of bacterial infections) 43 , aurintricarboxylic acid (inhibitor of RNA enzymes) 44 , nalidixic acid 45 and ibuprofen (anti-inflamatory drug) 46 . As far as the free receptor is concerned, quite clearly 1 adopts a quasi perfect 'cone' conformation in solution as reflected in the difference observed in the chemical shifts of the axial and equatorial protons (ax-eq = 1.03 ppm).…”

Section: Interaction Of 1 With Pharmaceuticalsmentioning

confidence: 99%

A calix[4]arene derivative and its selective interaction with drugs (clofibric acid, diclofenac and aspirin)

Namor

Nuaim

Salas

et al. 2017

European Journal of Pharmaceutical Sciences

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Nalidixic Acid-Resistant Strains of Salmonella Showing Decreased Susceptibility to Fluoroquinolones in the Midwestern Region of the Republic of Ireland Due to Mutations in the gyrA Gene

Cited by 19 publications

References 13 publications

Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

Genomic Characterization of a Nalidixic Acid-Resistant Salmonella Enteritidis Strain Causing Persistent Infections in Broiler Chickens

A calix[4]arene derivative and its selective interaction with drugs (clofibric acid, diclofenac and aspirin)

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