Nandrolone decanoate determines cardiac remodelling and injury by an imbalance in cardiac inflammatory cytokines and ACE activity, blunting of the Bezold–Jarisch reflex, resulting in the development of hypertension

Franquni, João Vicente Maggioni; Nascimento, Andrews Marques do; Lima, Ewelyne Miranda de; Brasil, Girlândia Alexandre; Heringer, Otávio Arruda; Cassaro, Karla Oliveira dos Santos; Cunha, Thony Vinicius Pita da; Musso, Carlos Guido; Santos, Maria Carmen Lopes Ferreira Silva; Kalil, Ieda Carneiro; Endringer, Denise Coutinho; Boëchat, Giovanna Assis Pereirra; Bissoli, Nazaré Souza; Andrade, Tadeu Uggere de

doi:10.1016/j.steroids.2012.12.009

Cited by 56 publications

(62 citation statements)

References 40 publications

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“…(24) showed that chronic administration of nandrolone can lead to significant increase in HW to BW ratio. They showed that activity of angiotensin-converting enzyme during consumption of nandrolone increases and this can lead to increase of angiotensin II release, which is a factor for hypertrophic induction.…”

Section: Discussionmentioning

confidence: 99%

Nandrolone plus moderate exercise increases the susceptibility to lethal arrhythmias

Joukar¹,

Baravati²,

Fathpour³

et al. 2015

Res Cardiovasc Med

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Background: Until now, no experimental study has directly assessed the arrhythmogenesis of chronic consumption of anabolic androgenic steroids along with moderate-intensity endurance exercise. Objectives: We evaluated the influence of integration of anabolic androgenic steroids along with moderate-intensity endurance exercise on susceptibility to lethal ventricular arrhythmias in rat. Materials and Methods:The animal groups were as follows: control group (CTL); exercise group (EX) which were under 6 weeks of treadmill exercise; nandrolone group (Nan) which received 5 mg/kg of nandrolone decanoate twice a week; vehicle group (Arach) which received Arachis oil (solvent of nandrolone); trained vehicle group (Arach + Ex); and trained nandrolone group (Nan + Ex). One day after ending of the intervention period, arrhythmia was inducted by intravenous infusion of aconitine and ventricular arrhythmias were recorded. Then malondialdehyde (MDA) and glutathione peroxidase (GPX) of heart tissue were measured. Results: Nandrolone, exercise, and their combination were associated with heart hypertrophy. Exercise could prevent the incremental effect of nandrolone on MDA/GPX ratio. Chronic administration of nandrolone with moderate-intensity endurance exercise had no significant effect on blood pressure, heart rate, and basal electrocardiographic parameters. Combination of nandrolone and exercise significantly increased the incidence of ventricular fibrillation (VF) and reduced the VF latency (P < 0.05). Conclusions:The findings suggest that chronic coadministration of nandrolone with moderate-intensity endurance exercise facilitates the VF occurrence in rat. Complementary studies are needed to elucidate the involved mechanisms of this abnormality.

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Section: Discussionmentioning

confidence: 99%

Nandrolone plus moderate exercise increases the susceptibility to lethal arrhythmias

Joukar¹,

Baravati²,

Fathpour³

et al. 2015

Res Cardiovasc Med

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“…The serum was kept at -80ºC until analysis. The evaluation of ACE activity in serum was performed with a colorimetric assay as described previously [Franquni et al 2013], with the following modifications; instead of cardiac homogenate, 10 µl of serum was used. For the evaluation of cardiac hypertrophy, the hearts were removed, washed with saline, dried with filter paper and weighed.…”

Section: Evaluation Of Ace Activity On Serum and Histological Analysesmentioning

confidence: 99%

“…For the evaluation of cardiac hypertrophy, the hearts were removed, washed with saline, dried with filter paper and weighed. The histological analyses for cardiac hypertrophy determination were evaluated by the number of myocyte nuclei per high power field, area of the cardiac myocyte nuclei (µm 2 ), and heart weight to body weight ratio (HW/BW) [Franquni et al 2013].…”

Section: Evaluation Of Ace Activity On Serum and Histological Analysesmentioning

confidence: 99%

Phytochemical and in vitro and in vivo biological investigation on the antihypertensive activity of mango leaves (Mangifera indica L.)

Ronchi

Brasil

Nascimento

et al. 2015

Therapeutic Advances in Cardiovascular Disease

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Aims:The aim of this study was to investigate the antihypertensive effect of leaves Mangifera indica L. using in vitro and in vivo assays. Methodology: The ethanol extract of leaves of M. indica was fractionated to dichloromethanic, n-butyl alcohol and aqueous fractions. The chemical composition of ethanolic extract and dichloromethanic fraction were evaluated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Antioxidant activity was evaluated in the DPPH scavenging activity assay. Angiotensin-converting enzyme (ACE) inhibitory activity was investigated using in vitro and in vivo assays. The chronic antihypertensive assay was performed in spontaneously hypertensive rats (SHRs) and Wistar rats treated with enalapril (10 mg/kg), dichloromethanic fraction (100 mg/kg; twice a day) or vehicle control for 30 days. The baroreflex sensitivity was evaluated through the use of sodium nitroprusside and phenylephrine. Cardiac hypertrophy was evaluated by morphometric analysis. Results: The dichloromethanic fraction exhibited the highest flavonoid, total phenolic content and high antioxidant activity. Dichloromethanic fraction elicited ACE inhibitory activity in vitro (99 ± 8%) similar to captopril. LC-MS/MS analysis revealed the presence of ferulic acid (48.3 ± 0.04 µg/g) caffeic acid (159.8 ± 0.02 µg/g), gallic acid (142.5 ± 0.03 µg/g), apigenin (11.0 ± 0.01 µg/g) and quercetin (203.3 ± 0.05 µg/g). The chronic antihypertensive effects elicited by dichloromethanic fraction were similar to those of enalapril, and the baroreflex sensitivity was normalized in SHR. Plasma ACE activity and cardiac hypertrophy were comparable with animals treated with enalapril. Conclusions: Dichloromethanic fraction of M. indica presented an antihypertensive effect, most likely by ACE inhibition, with benefits in baroreflex sensitivity and cardiac hypertrophy. Altogether, the results of the present study suggest that the dichloromethanic fraction of M. indica leaves may have potential as a promoting antihypertensive agent.

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“…For the determination of the kinetic parameters, the enzyme concentration was chosen to hydrolyze less than 5% of the substrate present per unit time in order to obtain the initial rate. To correct for the inner filter effect we used an adjusting equation determined experimentally for 0.1 to 100 μM Abz-FR-OH, used as standard for fluorescence measurements 15 . The ACE activity was expressed in uF.min …”

Section: Training Protocolmentioning

confidence: 99%

“…Previous studies have also demonstrated that administration of AAS inhibits the activity of matrix metalloproteinases (MMPs) and collagen gene expression, possibly impairing tendon 12,13 , and cardiac remodeling 14 . Recently, were investigated 15 the effects of nandrolone on the ACE activity, cardiac cytokines profile and other biomarkers. It was observed that the use of nandrolone increased the ACE activity, reduced the cytokines anti-inflammatory and increased of matrix type I collagen deposition, demonstrating that the use of AAS may cause cardiac hypertrophy associated to arterial hypertension.…”

Section: Introductionmentioning

confidence: 99%

Nandrolone increases angiotensin-I converting enzyme activity in rats tendons

Marqueti

Hashimoto

Durigan

et al. 2015

Rev Bras Med Esporte

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Introduction: The renin-angiotensin system (RAS) has been associated with several biological processes of the human body, regulating, among others blood pressure and water and electrolytes balance. Moreover, RAS also regulates connective tissue growth. Recently, studies have shown that the use of nandrolone modifies the angiotensin-I converting enzyme (ACE) activity and increases collagen deposition in the heart. Objective: The aim of study was to evaluate the Angiotensin-I converting enzyme (ACE) activity in the superficial flexor tendon (SFT) and in serum after load exercise in combination with anabolic androgenic steroid (AAS) administration after training session and six weeks of detraining. Methods: Forty-eight Wistar rats were used into two groups (G1 and G2) subdivided into four subgroups: Sedentary (S); trained (T); AAS-treated (Deca-Durabolin

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Nandrolone decanoate determines cardiac remodelling and injury by an imbalance in cardiac inflammatory cytokines and ACE activity, blunting of the Bezold–Jarisch reflex, resulting in the development of hypertension

Cited by 56 publications

References 40 publications

Nandrolone plus moderate exercise increases the susceptibility to lethal arrhythmias

Nandrolone plus moderate exercise increases the susceptibility to lethal arrhythmias

Phytochemical and in vitro and in vivo biological investigation on the antihypertensive activity of mango leaves (Mangifera indica L.)

Nandrolone increases angiotensin-I converting enzyme activity in rats tendons

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