Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water

Ding, Chengshi; Jin, Min; Ma, Jing; Chen, Zhaoli; Shen, Zhiqiang; Yang, Dong; Shi, Danyang; Liu, Weili; Kang, Meiling; Wang, Jingfeng; Lǐ, Jùnwén; Qiu, Zhigang

doi:10.1016/j.jhazmat.2020.124224

Cited by 36 publications

(14 citation statements)

References 48 publications

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“…Different from the Ag NPs group, the membranes were atrophic and plicated instead of broken, which might be on account of the endogenous death process. As reported in our previous studies [ 16 , 17 , 46 ], it had been proved that nanomaterials could promote the transfer of antibiotic resistance genes by conjugation and transduction due to the increase of membrane permeability. Therefore, the decline in contact frequency and membrane broken degree was an ecological friendly characteristic of Lig-Ag NPs.…”

Section: Resultsmentioning

confidence: 77%

Combined bactericidal process of lignin and silver in a hybrid nanoparticle on E. coli

Ran

Li²,

Hao

et al. 2022

Adv Compos Hybrid Mater

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Among multiple engineered nanoparticles that have been used in the bactericidal application, silver nanoparticles (Ag NPs) are the most explored bactericidal functional materials with their high efficiency and broad-spectrum bactericidal properties. However, environmental toxicology and lack of modifiability restrict their further development. In this study, a simple and economic method was established to fabricate lignin and silver hybrid nanoparticles (Lig-Ag NPs) with bactericidal ability. Afterwards, material characterization, bactericidal evaluation, and mechanism exploration were implemented to explore the properties of Lig-Ag NPs. The results indicated that Lig-Ag NPs not only demonstrated remarkable dispersity, uniformity, and encapsulation efficiency but also possessed approximated bactericidal ability on Escherichia coli and better durability compared with the same concentration of Ag NPs on E. coli . On the other hand, flow cytometry and transcriptomic analysis were used to further explore the bactericidal mechanism of Lig-Ag NPs. The results showed that oxidative stress was the possible leading bactericidal mechanism of Lig-Ag NPs. The formation approaches of reactive oxygen species production were various including the slow release of silver ion and generation of quinone/semi-quinone radicals on account of the combined effect of lignin and silver. Graphical abstract Lig-Ag NPs exhibited remarkable dispersity, uniformity, encapsulation efficiency, and possessed approximated bactericidal ability and better durability compared with Ag NPs. Supplementary information The online version contains supplementary material available at 10.1007/s42114-022-00460-z.

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Section: Resultsmentioning

confidence: 77%

Combined bactericidal process of lignin and silver in a hybrid nanoparticle on E. coli

Ran

Li²,

Hao

et al. 2022

Adv Compos Hybrid Mater

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“…Remarkably, the authors did not find any effect on conjugation frequency when bacteria were exposed to the same concentration of bulk alumina, suggesting a NP-specific effect [80]. Likewise, several studies have described enhanced conjugation transfer of ARGs in bacteria exposed to subinhibitory concentrations of Al2O3-NPs, ZnO-NPs, TiO2-NPs, and CuO-NPs, whereas bulk or ionic CuO, Al2O3, and TiO2 did not [80,[84][85][86]89].…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 88%

“…On the other hand, metallic NPs could promote horizontal transference of ARGs between bacteria. Over the last years, several studies have found that exposure to sub-inhibitory levels of metallic NPs favors bacterial conjugation and transformation in laboratory cultures [80,82,84,85], natural environments [62,86], and human-made systems [87]. Different laboratories recently observed higher transformation frequency when bacteria were exposed to either ZnO-NP [86] or Al2O3-NPs [88].…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

et al. 2021

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The rapid spread of antibiotic resistances among bacteria demands novel strategies for infection control, and metallic nanoparticles appear as promising tools because of their unique size and tunable properties that allow their antibacterial effects to be maximized. Furthermore, their diverse mechanisms of action towards multiple cell components have suggested that bacteria could not easily develop resistance against nanoparticles. However, research published over the last decade has proven that bacteria can indeed evolve stable resistance mechanisms upon continuous exposure to metallic nanoparticles. In this review, we summarize the currently known individual and collective strategies employed by bacteria to cope with metallic nanoparticles. Importantly, we also discuss the adverse side effects that bacterial exposure to nanoparticles may have on antibiotic resistance dissemination and that might constitute a challenge for the implementation of nanoparticles as antibacterial agents. Overall, studies discussed in this review point out that careful management of these very promising antimicrobials is necessary to preserve their efficacy for infection control.

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“…19−21 However, this gene exchange gives ARGs an opportunity to spread and further develop in the environment. 22,23 Antibiotic-sensitive bacteria can obtain ARGs through HGT to form new ARB, while ARB obtain ARGs through HGT to form multi-drug-resistant bacteria (MDRB), including Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter. 24 This creates significant obstacles when treating pathogenic bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

“…Under normal circumstances, ARB replicate ARGs into their offspring through vertical gene transfer (VGT); this proliferation maintains the genetic stability of the population . However, in many cases, bacteria also exchange genes through horizontal gene transfer (HGT) to promote gene diversity. − However, this gene exchange gives ARGs an opportunity to spread and further develop in the environment. , Antibiotic-sensitive bacteria can obtain ARGs through HGT to form new ARB, while ARB obtain ARGs through HGT to form multi-drug-resistant bacteria (MDRB), including Enterococcus faecalis , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Enterobacter . This creates significant obstacles when treating pathogenic bacterial infections. , …”

Section: Introductionmentioning

confidence: 99%

Traditional and Emerging Water Disinfection Technologies Challenging the Control of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes

Cai

Sun

et al. 2021

ACS EST Eng.

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The emergence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment has created obstacles when treating infectious diseases with antibiotics. Wastewater treatment plants (WWTPs) serve as reservoirs for ARB and ARGs and can disseminate them into the environment. It is important to understand and address these risks. Generally, professional disinfection processes have been used in WWTPs to disinfect the target water body, with the goal of eliminating pathogenic microorganisms in the water. However, ARGs are not generally considered, and antibiotic resistance has spread and developed through horizontal gene transfer (HGT). This Review provides a detailed overview of the application progress of different traditional and new disinfection technologies in removing ARB and ARGs, mainly focusing on the bacterial inactivation mechanisms of chlorination, ozonation, ultraviolet (UV) (including UVA, UVB, and UVC), sunlight, sunlight-dissolved organic matter (DOM), and photocatalysis (PC)/photoelectrocatalysis (PEC). In addition, this Review also focuses on the disinfection technology involved in the transfer of ARGs and clarifies the underlying transfer mechanisms in water environments. Furthermore, by linking the mechanisms of bacterial inactivation, the Review describes how SOS response and cell membrane permeability may be the key step in the conjugation, transformation, and transduction of ARGs. Finally, given the applications and current problems associated with traditional water disinfection technologies and light-based disinfection technologies in removing and controlling ARB and ARGs, this Review describes the current challenges and opportunities to facilitate the development of future disinfection technologies. The Review also highlights future research directions related to ARG transmission control.

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Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water

Cited by 36 publications

References 48 publications

Combined bactericidal process of lignin and silver in a hybrid nanoparticle on E. coli

Combined bactericidal process of lignin and silver in a hybrid nanoparticle on E. coli

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

Traditional and Emerging Water Disinfection Technologies Challenging the Control of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes

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