Nano‐ and Microstructured ECM and Biomimetic Scaffolds for Cardiac Tissue Engineering

Jallerat, Quentin; Szymanski, John M.; Feinberg, Adam W.

doi:10.1002/9781118843499.ch12

Cited by 3 publications

(6 citation statements)

References 140 publications

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“…Further, 3D muscle constructs more readily provide functional metrics of skeletal muscle maturity, such as twitch force and calcium handling dynamics. While it is challenging to precisely micropattern LAM cues in 3D, emerging technologies such as decellualrization 28, 34 and engineered LAM nanofibers 17, 47 suggest that we may be able to engineer 3D scaffolds comparable to our 2D surfaces in the near future.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle

2016

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Skeletal muscle lost through trauma or disease has proven difficult to regenerate due to the challenge of differentiating human myoblasts into aligned, contractile tissue. To address this, we investigated microenvironmental cues that drive myoblast differentiation into aligned myotubes for potential applications in skeletal muscle repair, organ-on-chip disease models and actuators for soft robotics. We used a 2D in vitro system to systematically evaluate the role of extracellular matrix (ECM) protein composition and geometric patterning for controlling the formation of highly aligned myotubes. Specifically, we analyzed myotubes differentiated from murine C2C12 cells and human skeletal muscle derived cells (SkMDCs) on micropatterned lines of laminin compared to fibronectin, collagen type I, and collagen type IV. Results showed that laminin supported significantly greater myotube formation from both cells types, resulting in greater than 2-fold increase in myotube area on these surfaces compared to the other ECM proteins. Species specific differences revealed that human SkMDCs uniaxially aligned over a wide range of micropatterned line dimensions, while C2C12s required specific line widths and spacings to do the same. Future work will incorporate these results to engineer aligned human skeletal muscle tissue in 2D for in vitro applications in disease modeling, drug discovery and toxicity screening.

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Section: Discussionmentioning

confidence: 99%

Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle

2016

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“…As observed from Table , tensile strength of PCLF:PCL nanofibrous scaffolds prepared with 5%w/w BAPO is close to the mammalian left ventricle (0.15 MPa in a canine model) and Young's modulus value is in the range of Young's modulus of rat and human myocardium (10 kPa to 1 MPa). Furthermore, the PCLF:PCL nanofibrous scaffold showed elastic properties in the frequency range from 0.6 to 2 Hz which is in the range of at the rest heart frequency until physiological frequency interval of beating heart . Therefore, it can be suggested that the produced substrate can be a potential candidate for the soft heart tissue.…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, the PCLF:PCL nanofibrous scaffold showed elastic properties in the frequency range from 0.6 to 2 Hz which is in the range of at the rest heart frequency until physiological frequency interval of beating heart. 20 Therefore, it can be suggested that the produced substrate can be a potential candidate for the soft heart tissue. Of course, it is worth noting that investigation this capability requires supplementary in vitro and in vivo testing.…”

Section: Discussionmentioning

confidence: 99%

“…Nanofibrous scaffolds are one of the scaffold types which have been used for soft tissue engineering applications. Such scaffolds benefit from high surface area to volume ratio and dimensional similarity with native extracellular matrix, making them an interesting substrate for tissue engineering applications . Based on the importance of nanofibrous elastomeric scaffolds in soft tissue regeneration, this study aimed to synthesize PCLF and fabricate it to PCLF nanofibrous scaffolds.…”

Section: Introductionmentioning

confidence: 99%

“…Such scaffolds benefit from high surface area to volume ratio and dimensional similarity with native extracellular matrix, making them an interesting substrate for tissue engineering applications. [17][18][19][20][21][22] Based on the importance of nanofibrous elastomeric scaffolds in soft tissue regeneration, this study aimed to synthesize PCLF and fabricate it to PCLF nanofibrous scaffolds. Due to the lack of spinnability of PCLF, PCL was further blended with PCLF in different ratios and PCLF:PCL nanofibrous scaffolds were fabricated by electrospinning.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering

et al. 2017

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With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented. Sol fraction results, Fourier-transform infrared spectroscopy, and mechanical tests confirmed occurrence of crosslinking reaction. Strain at break and Young's modulus of crosslinked PCLF:PCL nanofibers fabricated was found to be 114.5 ± 3.9% and 0.6 ± 0.1 MPa, respectively, and dynamic mechanical analysis results revealed elasticity of nanofibers. MTS assay showed biocompatibility of PCLF:PCL (70:30) nanofibrous scaffolds. Our overall results showed that electrospun PCLF:PCL nanofibrous scaffold could be considered as a candidate for further in vitro and in vivo experiments and its application for engineering of soft tissues subjected to in vivo cyclic mechanical stresses. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2371-2383, 2018.

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Biological Soft Robotics

Feinberg

2015

Annu. Rev. Biomed. Eng.

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In nature, nanometer-scale molecular motors are used to generate force within cells for diverse processes from transcription and transport to muscle contraction. This adaptability and scalability across wide temporal, spatial, and force regimes have spurred the development of biological soft robotic systems that seek to mimic and extend these capabilities. This review describes how molecular motors are hierarchically organized into larger-scale structures in order to provide a basic understanding of how these systems work in nature and the complexity and functionality we hope to replicate in biological soft robotics. These span the subcellular scale to macroscale, and this article focuses on the integration of biological components with synthetic materials, coupled with bioinspired robotic design. Key examples include nanoscale molecular motor-powered actuators, microscale bacteria-controlled devices, and macroscale muscle-powered robots that grasp, walk, and swim. Finally, the current challenges and future opportunities in the field are addressed.

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Nano‐ and Microstructured ECM and Biomimetic Scaffolds for Cardiac Tissue Engineering

Cited by 3 publications

References 140 publications

Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle

Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle

Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering

Biological Soft Robotics

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