Nano-Aptasensing in Mycotoxin Analysis: Recent Updates and Progress

Rhouati, Amina; Bülbül, Gonca; Latif, Usman; Hayat, Akhtar; Li, Zhanhong; Marty, Jean Louis

doi:10.3390/toxins9110349

Cited by 50 publications

(28 citation statements)

References 150 publications

(158 reference statements)

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“…Analytical performance results proved that the method adopted was fit for the determination of OTA in the analysed samples, and generated reliable results. Chromatography is the most commonly used method for mycotoxin analysis in food and feed, although alternative fast, easy to use and cheap technologies have recently been developed, such as analytical methods based on biosensors [30]. However, in the case of the analysis of bile for the detection of OTA, the analytical method adopted is fast and cheap.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Ochratoxin A Exposure in Ornamental and Self-Consumption Backyard Chickens

Guerrini

Altafini

Roncada

2020

Veterinary Sciences

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Ochratoxin A (OTA) is a mycotoxin that may be present in various food and feed of plant and animal origin, including chicken meat. In Italy, backyard poultry farming is rather widespread. Animals are raised for meat, eggs and for ornamental purpose, and they are often fed with home-made diets not subject to official controls. The purpose of this study was to evaluate exposure of ornamental and backyard chickens to OTA using biliary ochratoxin A as a biomarker. Therefore, bile samples, in addition to kidney, liver and muscle, were collected from 102 chickens reared in 16 farms located in 6 Italian regions. High-performance liquid chromatography method and fluorimetric detection (HPLC-FLD) analysis were carried out firstly on bile from all animals, and OTA was detected in 12 chickens (concentration range 3.83–170.42 µg/L). Subsequently, the kidneys of these chickens were also analysed, and the mycotoxin was not detected. The analytical detection limits (LODs) of OTA in bile and kidney were 2.1 µg/L and 0.1 µg/kg, respectively. In conclusion, these animals were exposed to OTA but their meat can be considered safe, given that this mycotoxin, if present, concentrates highest in kidneys. Biliary ochratoxin A confirms its use as a valid biomarker to assess exposure of poultry to OTA.

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Section: Discussionmentioning

confidence: 99%

Assessment of Ochratoxin A Exposure in Ornamental and Self-Consumption Backyard Chickens

Guerrini

Altafini

Roncada

2020

Veterinary Sciences

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“…The reaction of aptamer with a target results in changing the environment resulted in optical or electrochemical response. In case of fluorescence aptasensors, changes in the intensity of emission can be observed both in homogeneous solutions and on the appropriate support [15,16]. Besides, the aptamers bearing labels can be folded in three-dimensional structures using specific interactions of partially hybridized items or auxiliary DNA/RNA sequences.…”

Section: Aptasensors-assembling and Signal Transductionmentioning

confidence: 99%

“…The performance of electrochemical aptasensors applied for mycotoxin determination is summarized in Table 4 for the period from 2014 to 2018. Earlier works have been discussed in reviews [11,16,145,146]. [176] As can be seen, modification of electrodes can alter the detectable concentrations of mycotoxins by several orders of magnitude.…”

Section: Mycotoxinmentioning

confidence: 99%

“…They are compatible with flow-through and microfluidics assay formats. Recently, some aspects of electrochemical biosensors for mycotoxin determination have been summarized in reviews [9,[16][17][18]. In this review, application of electrochemical biosensors based on affine interactions is considered for sensitive mycotoxin analysis with particular emphasis on the sensor interface assembly and real sample assay problems.…”

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confidence: 99%

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Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

Evtugyn

Hianik

2019

Chemosensors

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Modern analysis of food and feed is mostly focused on development of fast and reliable portable devices intended for field applications. In this review, electrochemical biosensors based on immunological reactions and aptamers are considered in the determination of mycotoxins as one of most common contaminants able to negatively affect human health. The characteristics of biosensors are considered from the point of view of general principles of bioreceptor implementation and signal transduction providing sub-nanomolar detection limits of mycotoxins. Moreover, the modern trends of bioreceptor selection and modification are discussed as well as future trends of biosensor development for mycotoxin determination are considered.

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“…To transform the mycotoxin interaction into a measurable analytical signal, different electrochemical techniques have been used like (1) amperometric, which measures the changes in the current at a given applied voltage resulting from the oxidation or reduction of an electroactive biological element providing specific quantitative analytical information; (2) potentiometric, which measures the changes in the voltage between the working and the reference electrodes due to the establishment of an electrostatic interaction; (3) conductometric, which measures the changes in the capability of the sensing material to transport charge (electron); and (4) impedimetric, which measures the resistance of the generated electric current at certain applied voltage and combines the analysis of both the resistive and capacitive properties of the materials [109][110][111][112][113]. Many review articles have focused on mycotoxin detection using different electrochemical biosensors [47,107,114,115] and on the application of nanomaterial-mediated bio and immunosensors [21,75,76].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

et al. 2019

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Mycotoxins are a group of secondary metabolites produced by different species of filamentous fungi and pose serious threats to food safety due to their serious human and animal health impacts such as carcinogenic, teratogenic and hepatotoxic effects. Conventional methods for the detection of mycotoxins include gas chromatography and high-performance liquid chromatography coupled with mass spectrometry or other detectors (fluorescence or UV detection), thin layer chromatography and enzyme-linked immunosorbent assay. These techniques are generally straightforward and yield reliable results; however, they are time-consuming, require extensive preparation steps, use large-scale instruments, and consume large amounts of hazardous chemical reagents. Rapid detection of mycotoxins is becoming an increasingly important challenge for the food industry in order to effectively enforce regulations and ensure the safety of food and feed. In this sense, several studies have been done with the aim of developing strategies to detect mycotoxins using sensing devices that have high sensitivity and specificity, fast analysis, low cost and portability. The latter include the use of microarray chips, multiplex lateral flow, Surface Plasmon Resonance, Surface Enhanced Raman Scattering and biosensors using nanoparticles. In this perspective, thin film sensors have recently emerged as a good candidate technique to meet such requirements. This review summarizes the application and challenges of thin film sensor devices for detection of mycotoxins in food matrices.

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Nano-Aptasensing in Mycotoxin Analysis: Recent Updates and Progress

Cited by 50 publications

References 150 publications

Assessment of Ochratoxin A Exposure in Ornamental and Self-Consumption Backyard Chickens

Assessment of Ochratoxin A Exposure in Ornamental and Self-Consumption Backyard Chickens

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

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