Nano-Biomimetics for Nano/Micro Tissue Regeneration

Singh, Dinesh; Singh, Deepti; Zo, Sunmi; Han, Sung Soo

doi:10.1166/jbn.2014.1941

Cited by 30 publications

(20 citation statements)

References 25 publications

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“…Binding between the polymerized Sil layer and fibrin/alginate layer seemed uniform for both the composites. As previously reported, one of the main features of the fibrin/alginate dermal scaffold Smart Matrix is the presence of both nanofibers and nanopores, thus resembling the natural extracellular matrix (ECM) which cells are exposed to in vivo . SEM suggests that these nanofeatures are still present in the two‐component hybrid scaffolds (Figure , images at 5000×, far right column).…”

Section: Resultssupporting

confidence: 53%

“…As previously reported, one of the main features of the fibrin/alginate dermal scaffold Smart Matrix is the presence of both nanofibers and nanopores, thus resembling the natural extracellular matrix (ECM) which cells are exposed to in vivo. [29,33] SEM suggests that these nanofeatures are still present in the two-component hybrid scaffolds (Figure 2 Confocal microscopy showed no qualitative differences in terms of surface topography between the fibrin/alginate scaffold and the two-component hybrid ones (Figure 3). Confocal microscopy images also showed consistent results in terms of structure for the three scaffolds: all scaffolds presented open and interconnected porous structures with micropores in a range of sizes.…”

Section: Structural Characterizationmentioning

confidence: 96%

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Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

Sharma

Kohli

Moulding

et al. 2017

Macromolecular Bioscience

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The aim of this study is to design a novel two-component hybrid scaffold using the fibrin/alginate porous hydrogel Smart Matrix combined to a backing layer of plasma polymerized polydimethylsiloxane (Sil) membrane to make the fibrin-based dermal scaffold more robust for the treatment of the clinically challenging pressure sores. A design criteria are established, according to which the Sil membranes are punched to avoid collection of fluid underneath. Manual peel test shows that native silicone does not attach to the fibrin/alginate component while the plasma polymerized silicone membranes are firmly bound to fibrin/alginate. Structural characterization shows that the fibrin/alginate matrix is intact after the addition of the Sil membrane. By adding a Sil membrane to the original fibrin/alginate scaffold, the resulting two-component scaffolds have a significantly higher shear or storage modulus G'. In vitro cell studies show that dermal fibroblasts remain viable, proliferate, and infiltrate the two-component hybrid scaffolds during the culture period. These results show that the design of a novel two-component hybrid dermal scaffold is successful according to the proposed design criteria. To the best of the authors' knowledge, this is the first study that reports the combination of a fibrin-based scaffold with a plasma-polymerized silicone membrane.

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Section: Resultssupporting

confidence: 53%

Section: Structural Characterizationmentioning

confidence: 96%

Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

Sharma

Kohli

Moulding

et al. 2017

Macromolecular Bioscience

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show abstract

“…The development of nanostructured materials has recently assumed great importance in cellular studies and regenerative medicine, since these materials may represent a smart solution to produce devices associated with significant human applications, manufactured at high production rates and volumes (for recent reviews, see e.g. Singh et al, ; Hopley et al , ; Hofmann, ; Fraczek‐Szczypta, ).…”

Section: Introductionmentioning

confidence: 99%

A peroxiredoxin-based proteinaceous scaffold for the growth and differentiation of neuronal cells and tumour stem cells in the absence of prodifferentiation agents

Cimini

Ardini

Gentile

et al. 2016

J Tissue Eng Regen Med

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The use of nanoscale materials in the design of scaffolds for CNS tissue is increasing, due to their ability to promote cell adhesion, to mimic an extracellular matrix microenvironment and to interact with neuronal membranes. In this framework, one of the major challenges when using undifferentiated neural cells is how to control the differentiation process. Here we report the characterization of a scaffold based on the self-assembled nanotubes of a mutant of the protein peroxiredoxin (from Schistosoma mansoni or Bos taurus), which allows the growth and differentiation of a model neuronal cell line (SHSY5Y). The results obtained demonstrate that SHSY5Y cells grow without any sign of toxicity and develop a neuronal phenotype, as shown by the expression of neuronal differentiation markers, without the use of any differentiation supplement, even in the presence of serum. The prodifferentiation effect is demonstrated to be dependent on the formation of the protein nanotube, since a wild-type (WT) form of the peroxiredoxin from Schistosoma mansoni does not induce any differentiation. The protein scaffold was also able to induce the spread of glioblastoma cancer stem cells growing in neurospheres and allowing the acquisition of a neuron-like morphology, as well as of immature rat cortical neurons. This protein used here as coating agent may be suggested for the development of scaffolds for tissue regeneration or anti-tumour devices. Copyright © 2016 John Wiley & Sons, Ltd.

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“…The ideal objective would be to restore the tendon to its pre-injured state. This is the goal of tissue engineering (TE) [15][16][17][18][19] of tendons, a growing field which aims to combine scaffolds, cells and growth factors to form constructs that will aid regeneration of the native tendon. This review will focus on the application of tissue engineering strategies for tendon regeneration, including the selection and design of scaffold materials, cell sources and growth factors that may overcome limitations of current methods used for tendon reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Tendon Reconstruction with Tissue Engineering Approach—A Review

Verdiyeva¹,

Koshy²,

Glibbery³

et al. 2015

j biomed nanotechnol

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Tendon injuries are a common and rising occurrence, associated with significant impairment to quality of life and financial burden to the healthcare system. Clinically, they represent an unresolved problem, due to poor natural tendon healing and the inability of current treatment strategies to restore the tendon to its native state. Tissue engineering offers a promising alternative, with the incorporation of scaffolds, cells and growth factors to support the complete regeneration of the tendon. The materials used in tendon engineering to date have provided significant advances in structural integrity and biological compatibility and in many cases the results obtained are superior to those observed in natural healing. However, grafts fail to reproduce the qualities of the pre-injured tendon and each has weaknesses subject to its constituent parts. Furthermore, many materials and cell types are being investigated concurrently, with seemingly little association or comparison between research results. In this review the properties of the most-investigated and effective components have been appraised in light of the surrounding literature, with research from early in-vitro experiments to clinical trials being discussed. Extensive comparisons have been made between scaffolds, cell types and growth factors used, listing strengths and weaknesses to provide a stable platform for future research. Promising future endeavours are also described in the field of nanocomposite material science, stem cell sources and growth factors, which may bypass weaknesses found in individual elements. The future of tendon engineering looks bright, with growing understanding in material technology, cell and growth factor application and encouraging recent advances bringing us ever closer to regenerating the native tendon.

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Nano-Biomimetics for Nano/Micro Tissue Regeneration

Cited by 30 publications

References 25 publications

Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

A peroxiredoxin-based proteinaceous scaffold for the growth and differentiation of neuronal cells and tumour stem cells in the absence of prodifferentiation agents

Tendon Reconstruction with Tissue Engineering Approach—A Review

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