Nano-characterization of two closely related melanoma cell lines with different metastatic potential

Gostek, Justyna; Prauzner-Bechcicki, Szymon; Nimmervoll, Benedikt; Mayr, K.; Pabijan, Joanna; Hinterdorfer, Peter; Chtcheglova, Lilia A.; Lekka, Małgorzata

doi:10.1007/s00249-014-1000-y

Cited by 17 publications

(14 citation statements)

References 24 publications

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“…In one study, authors reported that two closely related human melanoma cell lines with different metastatic potential (WM115 and WM266-4) differed in their stiffness with the more aggressive, designated as WM266-4, being slightly softer. The authors attributed these differences to the highly flexible ridges found on the surface of metastatic melanoma cells [ 26 ]. In another study, researchers showed that metastatic melanoma WM239A cells were actually stiffer than non-metastatic WM115 cells [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Nanomechanical Phenotype of Melanoma Cells Depends Solely on the Amount of Endogenous Pigment in the Cells

Sarna¹,

Czuba-Pelech²,

Urbańska³

2018

IJMS

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Cancer cells have unique nanomechanical properties, i.e., they behave as if they were elastic. This property of cancer cells is believed to be one of the main reasons for their facilitated ability to spread and metastasize. Thus, the so-called nanomechanical phenotype of cancer cells is viewed as an important indicator of the cells’ metastatic behavior. One of the most highly metastatic cancer cells are melanoma cells, which have a very unusual property: they can synthesize the pigment melanin in large amounts, becoming heavily pigmented. So far, the role of melanin in melanoma remains unclear, particularly the impact of the pigment on metastatic behavior of melanoma cells. Importantly, until recently the potential mechanical role of melanin in melanoma metastasis was completely ignored. In this work, we examined melanoma cells isolated from hamster tumors containing endogenous melanin pigment. Applying an array of advanced microscopy and spectroscopy techniques, we determined that melanin is the dominating factor responsible for the mechanical properties of melanoma cells. Our results indicate that the nanomechanical phenotype of melanoma cells may be a reliable marker of the cells’ metastatic behavior and point to the important mechanical role of melanin in the process of metastasis of melanoma.

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Section: Discussionmentioning

confidence: 99%

Nanomechanical Phenotype of Melanoma Cells Depends Solely on the Amount of Endogenous Pigment in the Cells

Sarna¹,

Czuba-Pelech²,

Urbańska³

2018

IJMS

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“…MDC staining was used to confirm the abundance of autophagic vacuoles in cells. CA-4-treated MCF-7 cells were stained with 50 μM MDC for 1 h at 37°C, and then examined by fluorescence microscopy or analyzed on a flow cytometer using Cell Quest software [11, 12]. …”

Section: Methodsmentioning

confidence: 99%

Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy

Chen

Liu

et al. 2017

PLoS ONE

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As an inhibitor of microtubule assembly, combretastatin A-4 (CA-4)-induced biological responses in tumor cells have been well known, but the corresponding changes in nano-biophysical properties were not investigated given the lack of an ideal tool. Using AFM technique, we investigated the alteration of nano-biophysical properties when CA-4-treated tumor cells underwent the different biological processes, including cell cycle arrest, apoptosis and autophagy. We found that CA-4-resistant cells were rougher with the presence of characteristic “ridges”, indicating that the development of “ridge” structure may be a determinant of the sensitivity of cells to CA-4 compounds. CA-4 induced G2/M arrest and apoptosis in sensitive cells but triggered anti-apoptotic autophagy in resistant cells. CA-4 treatment caused an increase in stiffness in both sensitive and resistant cells. However, these cells exhibited different changes in cell surface roughness. CA-4 decreased Ra and Rq values in sensitive cells but increased these values in resistant cells. The reorganization of F-actin might contribute to the different changes of nano-biophysical properties in CA-4-sensitive and–resistant cells. Our results suggest that cellular nano-biophysical properties, such as “ridges”, roughness and stiffness, could be applied as potential biomarkers for evaluating CA-4 compounds, and knowledge regarding how biological alterations cause changes in cellular nano-biophysical properties is helpful to develop a new high-resolution screening tool for anti-tumor agents.

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“…The Young's modulus values as a function of cell density and indentation depth (e, f, g). Adapted from [35]. .…”

Section: List Of Figuresmentioning

confidence: 99%

“…On the other hand, Gostek et al [35], who focused on the nano-characterization of two different melanoma cell lines with similar morphological appearance but different metastatic potential, namely, WM115 from vertical growth phase (VGP) and WM266-4 derived from metastasis to skin, demonstrated that the elastic modulus value is dependent of the indentation depth and cellular density. , and 500 (c) for living melanoma cells grown at low (a single cell) and high density (in semi-confluent monolayer conditions).…”

Section: Fundamentals and The State Of The Artmentioning

confidence: 99%

“…Starting from glycocalix and the cell membrane, the AFM probe indents the cortex, formed by a network of actin filaments, and finally feels deeper parts of the cell, such as the cytosol and several organelles. Therefore, the indentation depth determines the cell regions that are probed [35]. For small indentation depth, the mechanical response will be dominated by the cell membrane tension and the surface layers of F-actin filaments, while deeper indentations provide information of different cellular organelles, such as the cell nucleus.…”

Section: Mechanical Properties Of the Cellsmentioning

confidence: 99%

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Atomic force spectroscopy in melanoma and keratinocytes cells.

Reinoza¹

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Nano-characterization of two closely related melanoma cell lines with different metastatic potential

Cited by 17 publications

References 24 publications

Nanomechanical Phenotype of Melanoma Cells Depends Solely on the Amount of Endogenous Pigment in the Cells

Nanomechanical Phenotype of Melanoma Cells Depends Solely on the Amount of Endogenous Pigment in the Cells

Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy

Atomic force spectroscopy in melanoma and keratinocytes cells.

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