Nano-Hydroxyapatite Composite Scaffolds Loaded with Bioactive Factors and Drugs for Bone Tissue Engineering

Mo, Xiaojing; Zhang, Dianjian; Liu, Keda; Zhao, Xin; Li, Xiaoming; Wang, Wei

doi:10.3390/ijms24021291

Cited by 75 publications

(32 citation statements)

References 197 publications

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“…The use of scaffolds has been widespread in biomedical applications for tissue engineering, which involves designing implantable biomimetic structures of organs or in vivo microenvironments, ,, as well as for sustained/controlled drug release. ,,,,,− The recent literature has reported scaffolds prepared using synthetic materials (e.g., polymers/polymeric hydrogels) , or naturally derived biomaterials ,,− or a combination of both . These materials can exhibit high biocompatibility and biodegradability, and provide a suitable microenvironment for cell growth, proliferation and differentiation, and tissue regeneration enhancement …”

Section: Scaffoldsmentioning

confidence: 99%

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Zangabad

Rezvani

Tong

et al. 2023

ACS Appl. Bio Mater.

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Recent preclinical and clinical studies have focused on the active area of therapeutic peptides due to their high potency, selectivity, and specificity in treating a broad range of diseases. However, therapeutic peptides suffer from multiple disadvantages, such as limited oral bioavailability, short half-life, rapid clearance from the body, and susceptibility to physiological conditions (e.g., acidic pH and enzymolysis). Therefore, high peptide dosages and dose frequencies are required for effective patient treatment. Recent innovations in pharmaceutical formulations have substantially improved therapeutic peptide administration by providing the following advantages: long-acting delivery, precise dose administration, retention of biological activity, and improvement of patient compliance. This review discusses therapeutic peptides and challenges in their delivery and explores recent peptide delivery formulations, including micro/nanoparticles (based on lipids, polymers, porous silicon, silica, and stimuli-responsive materials), (stimuli-responsive) hydrogels, particle/hydrogel composites, and (natural or synthetic) scaffolds. This review further covers the applications of these formulations for prolonged delivery and sustained release of therapeutic peptides and their impact on peptide bioactivity, loading efficiency, and (in vitro/in vivo) release parameters.

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Section: Scaffoldsmentioning

confidence: 99%

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Zangabad

Rezvani

Tong

et al. 2023

ACS Appl. Bio Mater.

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“…Growth factors, synthetic peptides, vitamins (D 3 and K), drugs, and other bioactive substances were employed for bone regeneration in conjunction with nHAp scaffolds. 11 One study demonstrated that the sustained delivery of growth factors increased the production of factors linked to osteogenic differentiation in poly(lactide-co-glycolide) (PLGA) microspheres loaded with vascular endothelial growth factors and bone morphogenic protein-2 (BMP-2) containing collagen and n-HAp. 12 Recent research using nHAp and polydopamineassisted coating of BMP-2-derived peptides shows considerably greater in vitro bone MSC (BMSC) viability, alkaline phosphatase (ALP) activity, and gene expression assay results.…”

Section: Introductionmentioning

confidence: 99%

“…Combining biomaterials with osteogenic bioactive molecules has become a widely used approach for BTE because bioactive factors play a significant role in the process of bone regeneration. Growth factors, synthetic peptides, vitamins (D 3 and K), drugs, and other bioactive substances were employed for bone regeneration in conjunction with nHAp scaffolds . One study demonstrated that the sustained delivery of growth factors increased the production of factors linked to osteogenic differentiation in poly(lactide- co -glycolide) (PLGA) microspheres loaded with vascular endothelial growth factors and bone morphogenic protein-2 (BMP-2) containing collagen and n-HAp .…”

Section: Introductionmentioning

confidence: 99%

Cell-Instructive Mineralized Microenvironment Regulates Osteogenesis: A Growing SYMBIOSIS of Cell Biology and Biomaterials Engineering in Bone Tissue Regeneration

Holkar

Kale

Ingavle

2023

ACS Biomater. Sci. Eng.

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One of the objectives of bone tissue engineering is to produce scaffolds that are biocompatible, osteoinductive, and mechanically equivalent to the natural extracellular matrix of bone in terms of structure and function. Reconstructing the osteoconductive bone microenvironment into a scaffold can attract native mesenchymal stem cells and differentiate them into osteoblasts at the defect site. The symbiotic relationship between cell biology and biomaterial engineering could result in composite polymers containing the necessary signals to recreate tissue- and organ-specific differentiation. In the current work, drawing inspiration from the natural stem cell niche to govern stem cell fate, the cell-instructive hydrogel platforms were constructed by engineering the mineralized microenvironment. This work employed two different hydroxyapatite delivery strategies to create a mineralized microenvironment in an alginate–PEGDA interpenetrating network (IPN) hydrogel. The first approach involved coating of nano-hydroxyapatite (nHAp) on poly(lactide-co-glycolide) microspheres and then encapsulating the coated microspheres in an IPN hydrogel for a sustained release of nHAp, whereas the second approach involved directly loading nHAp into the IPN hydrogel. This study demonstrate that both direct encapsulation and a sustained release approach showed enhanced osteogenesis in target-encapsulated cells; however, direct loading of nHAp into the IPN hydrogel increased the mechanical strength and swelling ratio of the scaffold by 4.6-fold and 1.14-fold, respectively. In addition, the biochemical and molecular studies revealed improved osteoinductive and osteoconductive potential of encapsulated target cells. Being less expensive and simple to perform, this approach could be beneficial in clinical settings.

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“…It offers improved osseointegration, biocompatibility, and controlled drug release. It can also be used as coatings for implants and in biosensors for early disease detection [21].…”

Section: Introductionmentioning

confidence: 99%

Densification of nanocrystalline hydroxyapatite powder via sintering: enhancing mechanical properties for biomedical applications

Akram

Khan

Amjad

et al. 2023

Mater. Res. Express

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The effect of compaction load, sintering temperature and soaking time on the sinter-ability and densification of Nano crystalline hydroxyapatite (HA) was assessed. The compaction and sinter-ability of HA particles was done at three different compaction loads and temperatures ranging from 1 ton to 5 ton and 850 ⁰C to 1250 ⁰C, respectively. Compaction of the green pellets was best achieved at 5-ton compaction load and it’s percent green densification was up to 50% of the theoretical density of HA (3.16g/cm3). For sintered density, the best results were achieved at a temperature of 1250⁰C and a compaction load of 1 ton which were 98% of the theoretical density. Soaking time at these sintering temperatures was varied between 1 and 3 hours and was found that with the variation of soaking time from 3 hours to 1 hour, the sintered density decreased tremendously at 850⁰C from 85% to 50% whereas at higher temperatures the decrease in density was only 4 to 6%. The maximum hardness of 625 (+28) HV1 was obtained for HA sintered at 1250⁰C with a soaking time of 3 hours. Phase analyses were carried out using an X-Ray diffractometer. The HA phase was stable even at the highest sintering temperature of 1250⁰C and did not decompose into α tri-calcium phosphate (TCP) and β TCP. The grain size was reduced by decreasing soaking time and lowering sintering temperature.

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Nano-Hydroxyapatite Composite Scaffolds Loaded with Bioactive Factors and Drugs for Bone Tissue Engineering

Cited by 75 publications

References 197 publications

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Recent Advances in Formulations for Long-Acting Delivery of Therapeutic Peptides

Cell-Instructive Mineralized Microenvironment Regulates Osteogenesis: A Growing SYMBIOSIS of Cell Biology and Biomaterials Engineering in Bone Tissue Regeneration

Densification of nanocrystalline hydroxyapatite powder via sintering: enhancing mechanical properties for biomedical applications

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