Nano‐Optical Tweezers: Methods and Applications for Trapping Single Molecules and Nanoparticles

Kolbow, Joshua D.; Lindquist, Nathan C.; Ertsgaard, Christopher T.; Yoo, Daehan; Oh, Sang Hyun

doi:10.1002/cphc.202100004

Cited by 19 publications

(6 citation statements)

References 116 publications

(265 reference statements)

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“…First, the improvement of detection sensitivity and accuracy will lead to more precise characterization and classification of nanoparticles. Sensitivity can be improved by increasing laser intensity, although this might be constrained by the optical trapping of nanoparticles . Another practical approach is incorporating lasers with different wavelengths depending on the measurement target.…”

Section: Discussionmentioning

confidence: 99%

Nanofluidic Detection Platform for Simultaneous Light Absorption and Scattering Measurement of Individual Nanoparticles in Flow

Tsuyama,

Mawatari

2024

Anal. Chem.

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Characterization and quantification of plasmonic nanoparticles at the single particle level have become increasingly important with the advancements in nanotechnology and their application to various biological analyses including diagnostics, photothermal therapy, and immunoassays. While various nanoparticle detection methodologies have been developed and widely used, simultaneous measurement of light absorption and scattering from individual plasmonic nanoparticles in flow is still challenging. Herein, we describe a novel nanofluidic detection platform that enables simultaneous measurement of absorption and scattering signals from individual nanoparticles within a nanochannel. Our detection platform utilized optical diffraction phenomena by a single nanochannel as both a readout signal for photothermal detection and a reference light for interferometric scattering detection. Through the elucidation of the frequency effect on the detection performance and optimization of experimental conditions, we achieved the classification of gold and silver nanoparticles with a diameter of 20−60 nm at an average accuracy score of 82.6 ± 2.1% by measured data sets of absorption and scattering signals. Furthermore, we demonstrated the concentration determination of plasmonic nanoparticle mixtures using a trained Support vector machine (SVM) classifier. Our simple yet sensitive nanofluidic detection platform will be a valuable tool for the analysis of nanoparticles and their applications to chemical and biological assays.

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Section: Discussionmentioning

confidence: 99%

Nanofluidic Detection Platform for Simultaneous Light Absorption and Scattering Measurement of Individual Nanoparticles in Flow

Tsuyama,

Mawatari

2024

Anal. Chem.

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“…Since their discovery in 1970, optical tweezers have emerged as a tool to trap and manipulate nano and micrometer-sized material using a highly focused laser beam [ 23 , 24 , 25 ]. It is an extremely sensitive and precise instrument capable of manipulating objects and detecting their position with sub-nanometer precision and measuring forces with femtonewton (10–15 N) accuracy [ 26 , 27 ]. Now, they are finding applications in many fields of science, such as biology and chemistry, where they are used extensively in studying the unfolding of proteins [ 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Brownian Motion in Optical Tweezers, a Comparison between MD Simulations and Experimental Data in the Ballistic Regime

et al. 2023

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The four most popular water models in molecular dynamics were studied in large-scale simulations of Brownian motion of colloidal particles in optical tweezers and then compared with experimental measurements in the same time scale. We present the most direct comparison of colloidal polystyrene particle diffusion in molecular dynamics simulations and experimental data on the same time scales in the ballistic regime. The four most popular water models, all of which take into account electrostatic interactions, are tested and compared based on yielded results and resources required. Three different conditions were simulated: a freely moving particle and one in a potential force field with two different strengths based on 1 pN/nm and 10 pN/nm. In all cases, the diameter of the colloidal particle was 50 nm. The acquired data were compared with experimental measurements performed using optical tweezers with position capture rates as high as 125 MHz. The experiments were performed in pure water on polystyrene particles with a 1 μm diameter in special microchannel cells.

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“…Many conventional optical trapping methods already exist, some of which rely on using top-down and bottom-up methods such as optical nanocavities, photonic crystal waveguide cavities, or nanobeam cavities . Other methods are based on providing the trapping force via the gradient intensity of a tightly focused laser beam to a diffraction-limited beam waist within a fluid containing the NPs, such as nanooptical tweezer , and pulsed laser trap techniques, which vary in several aspects such as simplicity, efficiency, robustness, stability, and complexity of fabrication.…”

Section: Introductionmentioning

confidence: 99%

Strategy for Patterning Single Colloidal Gold Nanoparticles on Two Photon Polymerized and Functionalized Ultrathin Nanostructures

et al. 2023

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Local and deterministic trapping of single nanoparticles (NPs) has always been a challenging topic due to the difficulties faced at such a small particle size. These difficulties are concerned with the stability, simplicity, robustness, and efficiency of the used trapping technique. Here, we used two-photon polymerization (TPP) of a prefunctionalized photopolymer to obtain a nanometric polymer layer and selectively attract single colloidal gold NPs (AuNPs). Thanks to a deep photochemical study of the threshold energy, we identified a photopolymerization regime allowing one to tune the polymer size and immobilize single gold nanoparticles. This method is promising for the fabrication of single photon sources.

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Nano‐Optical Tweezers: Methods and Applications for Trapping Single Molecules and Nanoparticles

Cited by 19 publications

References 116 publications

Nanofluidic Detection Platform for Simultaneous Light Absorption and Scattering Measurement of Individual Nanoparticles in Flow

Nanofluidic Detection Platform for Simultaneous Light Absorption and Scattering Measurement of Individual Nanoparticles in Flow

Brownian Motion in Optical Tweezers, a Comparison between MD Simulations and Experimental Data in the Ballistic Regime

Strategy for Patterning Single Colloidal Gold Nanoparticles on Two Photon Polymerized and Functionalized Ultrathin Nanostructures

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