Nano-realgar suppresses lung cancer stem cell growth by repressing metabolic reprogramming

Yang, Feiran; Zhao, Yu-feng; Hu, Xiwen; Liu, Zong-Kai; Yu, Xiaodan; Li, Chang-yang; Li, Xiurong; Li, Huijie

doi:10.1016/j.gene.2021.145666

Cited by 21 publications

(22 citation statements)

References 39 publications

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“…The cell viability was measured by a MTT assay as previously described. 31 In brief, cells were seeded at a density of 1×10 4 cells/well and cultured in 96-well plates for 24h. The cells were then treated with different concentrations (40, 20, 10, 5, and 2.5 μM) of curcumin (purity>95%, Solarbio, Beijing, China) for 72 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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Identifying the Antitumor Effects of Curcumin on Lung Adenocarcinoma Using Comprehensive Bioinformatics Analysis

Yang¹,

Li²,

Hu³

et al. 2022

DDDT

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Background As the main component of turmeric ( Curcuma longa L .), curcumin is widely used in the treatment of various diseases. Previous studies have demonstrated that curcumin has great potential as a therapeutic agent, but the lack of understanding of the functional mechanism of the drug has hindered the widespread use of the natural product. In the present study, we used comprehensive bioinformatics analysis and in vitro experiments to explore the anti-tumor mechanism of curcumin. Materials and Methods LUAD mRNA expression data were obtained from TCGA database and differentially expressed genes (DEGs) were identified using R software. Functional enrichment analysis was conducted to further clarify its biological properties and hub genes were identified by a protein–protein interaction (PPI) network analysis. Survival analysis and molecular docking were used to analyze the effectiveness of the hub genes. By an in vitro study, we evaluated whether curcumin could influence the proliferation, migration, and invasion activities of LUAD cells. Results In this study, 1783 DEGs from LUAD tissue samples compared to normal samples were evaluated. Functional enrichment analysis and the PPI network revealed the characteristics of the DEGs. We performed a topological analysis and identified 10 hub genes. Of these, six genes ( INS, GCG, SST, F2, AHSG, and NPY ) were identified as potentially effective biomarkers of LUAD. The molecular docking results indicated that curcumin targets in regulating lung cancer may be INS and GCG . We found that curcumin significantly inhibited the proliferation, migration, and invasion of LUAD cells and significantly decreased the expression of the INS and GCG genes. Conclusion The results of this study suggest that the therapeutic effects of curcumin on LUAD may be achieved through the intervention of INS and GCG , which may act as potential biomarkers for LUAD prevention and treatment.

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Section: Methodsmentioning

confidence: 99%

“…qRT-PCR was performed as previously described. 31 The sequences used were as follows: INS , forward: 5′- TGCATCAGAAGAGGCCATCA-3′, reverse: 5′- CGTTCCCCGCACACTAGGTA-3′; GCG , forward: 5′- TCCGATCTGACATATCTGCATT-3′, reverse: 5′- CACCACTGTGGCTACCAGTTC-3′. The Total RNA Kit I (OMEGA Biotek, GA, USA) was used to extract total RNA.…”

Section: Methodsmentioning

confidence: 99%

Identifying the Antitumor Effects of Curcumin on Lung Adenocarcinoma Using Comprehensive Bioinformatics Analysis

Yang¹,

Li²,

Hu³

et al. 2022

DDDT

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“…More recently, the effect of realgar nanoparticles on lung cancer stem cell was also examined. The nano-realgar was shown to inhibit tumor growth both in vitro and in vivo by repressing metabolic reprogramming via downregulation of HIF-1α expression and PI3K/Akt/mTOR pathway (Yang et al 2021 ).…”

Section: Inorganic and Organic Arsenic-containing Drugsmentioning

confidence: 99%

Arsenic in medicine: past, present and future

et al. 2022

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Arsenicals are one of the oldest treatments for a variety of human disorders. Although infamous for its toxicity, arsenic is paradoxically a therapeutic agent that has been used since ancient times for the treatment of multiple diseases. The use of most arsenic-based drugs was abandoned with the discovery of antibiotics in the 1940s, but a few remained in use such as those for the treatment of trypanosomiasis. In the 1970s, arsenic trioxide, the active ingredient in a traditional Chinese medicine, was shown to produce dramatic remission of acute promyelocytic leukemia similar to the effect of all- trans retinoic acid. Since then, there has been a renewed interest in the clinical use of arsenicals. Here the ancient and modern medicinal uses of inorganic and organic arsenicals are reviewed. Included are antimicrobial, antiviral, antiparasitic and anticancer applications. In the face of increasing antibiotic resistance and the emergence of deadly pathogens such as the severe acute respiratory syndrome coronavirus 2, we propose revisiting arsenicals with proven efficacy to combat emerging pathogens. Current advances in science and technology can be employed to design newer arsenical drugs with high therapeutic index. These novel arsenicals can be used in combination with existing drugs or serve as valuable alternatives in the fight against cancer and emerging pathogens. The discovery of the pentavalent arsenic-containing antibiotic arsinothricin, which is effective against multidrug-resistant pathogens, illustrates the future potential of this new class of organoarsenical antibiotics.

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Section: Npns Targeting Cscsmentioning

confidence: 99%

“…192 Moreover, realgar nanoparticles inhibited glucose metabolism, lung CSCs viability, and tumor growth by inhibiting metabolic reprogramming, which may be associated with the downregulation of HIF-1α expression via the PI3K/Akt/mTOR pathway. 193 Nanomaterials delivering triterpenoids such as anthothecol, nimbolide, and ginsenoside Rg3 similarly showed excellent ability to target CSCs. The use of nanoparticle graphene oxide loaded with ginsenoside Rg3 significantly inhibited the malignant progression of osteosarcoma, inhibited the sphere formation of CSCs, and improved the effect of photodynamic therapy.…”

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confidence: 99%

Natural Products-Based Nanoformulations: A New Approach Targeting CSCs to Cancer Therapy

Liao¹,

Li²,

Wang

et al. 2022

IJN

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Cancer stem cells (CSCs) lead to the occurrence and progression of cancer due to their strong tumorigenic, self-renewal, and multidirectional differentiation abilities. Existing cancer treatment methods cannot effectively kill or inhibit CSCs but instead enrich them and produce stronger proliferation, invasion, and metastasis capabilities, resulting in cancer recurrence and treatment resistance, which has become a difficult problem in clinical treatment. Therefore, targeting CSCs may be the most promising approach for comprehensive cancer therapy in the future. A variety of natural products (NP) have significant antitumor effects and have been identified to target and inhibit CSCs. However, pharmacokinetic defects and off-target effects have greatly hindered their clinical translation. NP-based nanoformulations (NPNs) have tremendous potential to overcome the disadvantages of NP against CSCs through site-specific delivery and by improving their pharmacokinetic parameters. In this review, we summarize the recent progress of NPNs targeting CSCs in cancer therapy, looking forward to transforming preclinical research results into clinical applications and bringing new prospects for cancer treatment.

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Nano-realgar suppresses lung cancer stem cell growth by repressing metabolic reprogramming

Cited by 21 publications

References 39 publications

Identifying the Antitumor Effects of Curcumin on Lung Adenocarcinoma Using Comprehensive Bioinformatics Analysis

Identifying the Antitumor Effects of Curcumin on Lung Adenocarcinoma Using Comprehensive Bioinformatics Analysis

Arsenic in medicine: past, present and future

Natural Products-Based Nanoformulations: A New Approach Targeting CSCs to Cancer Therapy

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