Nano to micron-sized particle detection in patients' lungs and its pathological significance

Forest, Valérie; Pourchez, Jérémie; Guibert, Christelle; Bitounis, Dimitrios; Leclerc, Lara; Sarry, Gwendoline; Vergnon, Jean‐Michel

doi:10.1039/c8en01301b

Cited by 9 publications

(18 citation statements)

References 27 publications

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“…However, regarding sarcoidosis, only half (54%) of the patients were classified in the group of high probability of exposure to unintentionally released nanoparticles. Once again, this observation is in agreement with our mineralogical analyses that previously highlighted a potential contribution of silica submicron particles, i.e., particles bigger than nanoparticles, in this disease [19].…”

Section: Discussionsupporting

confidence: 92%

“…We developed optimized protocols for each kind of biological matrix to isolate the micro, sub-micro, and nano fractions of various types of inorganic particles and thus perform comprehensive mineralogical analyses. We were thus able to determine the nanoparticle load in patients’ biological samples such as seminal and follicular fluids [ 15 ], colon [ 16 ], amniotic fluids [ 17 ], or BAL [ 18 , 19 , 20 ]. We especially focused our attention on these latter, as the biomonitoring of biopersitent nanoparticles in the lung could be particularly relevant in the case of respiratory diseases.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between Occupational Exposure to Airborne Nanoparticles, Nanoparticle Lung Burden and Lung Diseases

Forest

Pourchez

Pélissier

et al. 2021

Toxics

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The biomonitoring of nanoparticles in patients’ broncho-alveolar lavages (BAL) could allow getting insights into the role of inhaled biopersistent nanoparticles in the etiology/development of some respiratory diseases. Our objective was to investigate the relationship between the biomonitoring of nanoparticles in BAL, interstitial lung diseases and occupational exposure to these particles released unintentionally. We analyzed data from a cohort of 100 patients suffering from lung diseases (NanoPI clinical trial, ClinicalTrials.gov Identifier: NCT02549248) and observed that most of the patients showed a high probability of exposure to airborne unintentionally released nanoparticles (>50%), suggesting a potential role of inhaled nanoparticles in lung physiopathology. Depending on the respiratory disease, the amount of patients likely exposed to unintentionally released nanoparticles was variable (e.g., from 88% for idiopathic pulmonary fibrosis to 54% for sarcoidosis). These findings are consistent with the previously performed mineralogical analyses of BAL samples that suggested (i) a role of titanium nanoparticles in idiopathic pulmonary fibrosis and (ii) a contribution of silica submicron particles to sarcoidosis. Further investigations are necessary to draw firm conclusions but these first results strengthen the array of presumptions on the contribution of some inhaled particles (from nano to submicron size) to some idiopathic lung diseases.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Relationship between Occupational Exposure to Airborne Nanoparticles, Nanoparticle Lung Burden and Lung Diseases

Forest

Pourchez

Pélissier

et al. 2021

Toxics

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“…One of the earliest studies draws attention to workplace exposure to NPs where seven women developed serious lung issues after long-term occupational exposure to NPs and MPs of polyacrylate, silica, and silicates. These particles were found in their tissue samples and Bronchoalveolar lavage (BAL) but no firm conclusion of causation against the NP fractions was found (Kuhlbusch, Wijnhoven, and Haase 2018;Forest et al 2019). Asbestosis and silicosis are well-known lung pathologies caused by occupational exposure to inhaled dust and particles of bioresistant materials as asbestos, silica, and other minerals.…”

Section: Npsmentioning

confidence: 99%

“…The calls for co-implementing bio-monitoring is urgently needed where measuring the internal dose can reflect the deposited dose and assess the individual clinical picture. Some research studies are proposing approaches of monitoring the load of NPs in patients' samples and their clinical conditions and propose extracting these particles to be used for in vitro experiments to assess their toxicity; what is called in vivo to in vitro testing (Forest et al 2019). Other studies call for assessing different particles collected from different environmental or occupational settings and then studying their safety.…”

Section: Npsmentioning

confidence: 99%

Toxicological assessment of nanoparticle interactions with the pulmonary system

2019

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“…The assessment of the internal dose is a first step toward the characterization of persistent nanoparticles in tissues and the understanding of this potential source of adverse effects. With methodologies we specifically developed for that purpose, we have previously applied successfully this approach to the analysis of the nanoparticle burden in broncho-alveolar lavages [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Elemental fingerprint of human amniotic fluids and relationship with potential sources of maternal exposure

Raia-Barjat

Prieux

Leclerc

et al. 2020

Journal of Trace Elements in Medicine and Biology

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Background-The impact of nanoparticles we are increasingly exposed to remains largely unknown. Of particular concern is the exposure of pregnant women and potential impact on fetal development. Indeed, many in vitro and in vivo animal studies have shown that nanoparticles are able to cross the placental barrier and induce toxic effects to the fetus. However, little is known in humans. Objective-The aim and originality of this study were to investigate the nanoparticle burden of amniotic fluids in pregnant women. Methods-To that purpose, 100 amniotic fluids collected for clinical purposes were used to determine the nanoparticle quantity and nature by inductively coupled plasma atomic emission spectroscopy (NAMIOTIC, ClinicalTrials.gov Identifier: NCT02720887). Results-The prevalence of patients with a substantial concentration for the essential trace elements Cu, Fe and Zn was high, while that of patients with a substantial concentration of Al, Ag, Be, Co, Cr, Ni, Si, Ti and W was relatively low (under 20%). It was generally higher in the fraction containing nanoparticles and ions than in the fraction containing micro-and submicroparticles. No correlation was found between the nanoparticle burden and the different potential sources of exposure to nanoparticles (smoking status of the patient, living area, heating source, mode of transport, leisure, use of hygiene products and cosmetics and occupational activities). Conclusion-Our results showing low concentrations and low prevalence of most of the assessed elements in amniotic fluids are reassuring. Further research is needed to draw firm conclusions on the developmental toxicity of engineered nanoparticles in humans but the present paper can provide a useful basis for further evaluation of the fetal toxicity of nanoparticles.

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Nano to micron-sized particle detection in patients' lungs and its pathological significance

Abstract: Comprehensive mineralogical analyses (including nano to micron-sized particle detection) of patients' bronchoalveolar lavages allow obtaining new insights into the role of inhaled biopersistent particles in lung diseases.

Cited by 9 publications

References 27 publications

Relationship between Occupational Exposure to Airborne Nanoparticles, Nanoparticle Lung Burden and Lung Diseases

Relationship between Occupational Exposure to Airborne Nanoparticles, Nanoparticle Lung Burden and Lung Diseases

Toxicological assessment of nanoparticle interactions with the pulmonary system

Elemental fingerprint of human amniotic fluids and relationship with potential sources of maternal exposure

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