Nanocapsules with excellent biocompatibility and stability in protein solutions

Abstract

Silica nanocapsules (SiO2NCs) are usually prepared with cationic surfactants that are not cytocompatible. Dialysis can remove the surfactant but imparts detrimentally the stability of the SiO2NCs in the presence of...

Assembly of biomimetic microreactors using caged-coacervate droplets

“…34 The silica shell was formed at the interface of miniemulsion droplets stabilized with a reactive cationic surfactant, dimethyloctadecyl [3-(trimethoxysilyl)propyl] ammonium chloride (TPOAc), which can be hydrolyzed and co-condensed with TEOS to chemically bond to silica surfaces. 35 The diameters of SiO 2 NCs were controlled by varying the compositions of hydrophobic substances in the miniemulsion droplets. A volume ratio of 2 : 1 : 10 : 0 in cyclohexane (Cy), chloroform (CHCl 3 ), TEOS, and hexadecane (HD), respectively, was used to regulate the Ostwald ripening in the miniemulsion system, which yielded ultrasmall silica nanocapsules (ul-SiO 2 NCs) with a diameter about 10 nm (Fig.…”

Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

“…At the same time, the limitations of the use of nanomaterials also attracts the attention of researchers. Toxicity, stability, and biocompatibility are major problems hindering in vivo application, and when it comes to in vitro testing, stability is the primary consideration ( Ahmed et al, 2021 ; Jobdeedamrong et al, 2021 ; Li Y et al, 2021 ; Zhang Q et al, 2021 ). No matter how well designed, the deviation in repeated experiment results is too large, which leads to the greatly reduced reliability of such experimental results.…”

In Situ Measurement of Nanoparticle–Blood Protein Adsorption and Its Heterogeneity with Single-Nanoparticle Resolution via Dual Fluorescence Quantification

“…In the literature, adsorption of serum albumins was found to be effective to stabilize NPs prior to biological experiments. 38 The FL intensity showed no further increase from incubation with 2 mg mL −1 to 5 mg mL −1 of HSA, indicating the saturation of protein adsorption (Figure 2c).…”

“…In contrast, the fraction of these larger NPs was diminished as protein concentration increased to 2 mg mL –1 and 5 mg mL –1 , suggesting that increased protein adsorption enhanced colloidal stability of NPs. In the literature, adsorption of serum albumins was found to be effective to stabilize NPs prior to biological experiments . The FL intensity showed no further increase from incubation with 2 mg mL –1 to 5 mg mL –1 of HSA, indicating the saturation of protein adsorption (Figure c).…”