Nanocellulose biosynthesis by Komagataeibacter hansenii in a defined minimal culture medium

Souza, Samara Silva de; Berti, Fernanda Vieira; Oliveira, Karla Pollyanna Vieira de; Pittella, Camila Quinetti Paes; Castro, Julia V. de; Pelissari, Catiane; Rambo, Carlos R.; Porto, Luismar Marques

doi:10.1007/s10570-018-2178-4

Cited by 26 publications

(14 citation statements)

References 59 publications

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“…Com-pared to the HS medium, the improved production titer (3.3 ± 0.3 g/L BC) and reduced gluconic acid generation (7.2 ± 0.2 g/L) from MA/9 cultivations can be attributed to the buffering capacity in the growth medium [58]. Additionally, the presence of essential macro and micronutrients in the original MA/9 medium composition, may positively contribute to bacterial growth and cellular metabolism [59]. The obtained BC titers from the MA/9 medium are comparable to the previously published results from minimal media.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

et al. 2021

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Komagataeibacter spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.

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Section: Discussionmentioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

et al. 2021

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“…Improved buffering capacity is reasoned towards reduced gluconic acid formation in MA/9 cultivations (Lin et al 2016). HS medium lacks essential nutrients such as magnesium, calcium, iron and potassium ions, that play pivotal roles in cellular metabolism (de Souza et al 2019). Inclusion of such macro and micronutrients in the original MA/9 medium composition can positively effect the bacterial growth.…”

Section: Cellulose Production In Minimal Mediummentioning

confidence: 99%

Characterization of a novel bacterial cellulose producer for the production of eco-friendly piezoelectric-responsive films from a minimal medium containing waste carbon

et al. 2020

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Bacterial cellulose (BC) is a biodegradable polymer that benefits in purity, crystallinity and superior optical, structural and mechanical properties. Such properties facilitate BC to replace the conventional non-biodegradable materials used, for instance, in sensing applications. However, BC production is largely conducted in conventional medium containing model substrates and complex carbon-containing compounds. Aiming towards the production of eco-friendly piezoelectric-responsive BC films, we isolated and characterized a novel bacterial strain affiliated to Komagataeibacter rhaeticus. The K. rhaeticus ENS9a strain synthesized BC in minimal medium containing crude glycerol, generating a titer of 2.9 ± 0.3 g/L BC. This is, to the best of our knowledge, the highest BC titer reported from an unoptimized minimal medium containing crude glycerol. Interestingly, the films prepared from crude glycerol showed normal force and bending mode sensitivities of 6–11 pC/N and 40–71 pC/N, respectively, demonstrating a green platform to address both bioprocess waste valorization and implementation of cellulose-based alternatives for the non-sustainable and non-biodegradable materials, such as fluoropolymers or lead containing piezoceramics, used in sensing applications. In silico genome analysis predicted genes partaking in carbohydrate metabolism, BC biogenesis, and nitrogen fixation/regulation. Graphic abstract

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“…(2019) reported a titer of 0.22 g BC from K. hansenii ATCC 23769 grown in 10 ml of minimal medium containing glucose [59]. Recently, using a Komagataeibacter isolate cultivated in similar conditions and 20 g/L glucose as the carbon source we reported a titer of 2.2 ± 0.1 g/L BC [19].…”

Section: Discussionmentioning

confidence: 96%

“…Compared to the HS medium, the improved production titer (3.3±0.3 g/L BC) and reduced gluconic acid generation (7.2±0.2 g/L) from MA/9 cultivations can be attributed to the buffering capacity in the growth medium [58]. Additionally, the presence of essential macro and micronutrients in the original MA/9 medium composition, may positively contribute in bacterial growth and cellular metabolism [59]. The obtained BC titers from MA/9 medium are comparable to the previously published results from minimal media.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects on Waste Stream Valorization for Bacterial Cellulose Production

Cannazza¹,

Rissanen²,

Guizelini³

et al. 2021

Preprint

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Komagataeibacter spp. have been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose producing bacteria capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in minimal medium containing glucose (3.3±0.3 g/L), pure glycerol (2.2±0.1 g/L) and crude glycerol (2.1±0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in minimal medium supplemented with acetate, BC production was not observed. However, in presence of readily utilizable substrate, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.

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Nanocellulose biosynthesis by Komagataeibacter hansenii in a defined minimal culture medium

Cited by 26 publications

References 59 publications

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Characterization of a novel bacterial cellulose producer for the production of eco-friendly piezoelectric-responsive films from a minimal medium containing waste carbon

Characterization of Komagataeibacter Isolate Reveals New Prospects on Waste Stream Valorization for Bacterial Cellulose Production

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