Nanocellulose reinforced silkworm silk fibers for application to biodegradable polymers

Wu, Chen; Egawa, Satoshi; Kanno, Teruyoshi; Kurita, Hiroki; Wang, Zhenjin; Iida, Eiji; Narita, Fumio

doi:10.1016/j.matdes.2021.109537

Cited by 25 publications

(36 citation statements)

References 32 publications

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“…[23][24][25][26][27][28][29][30] Silk is the only natural ber that exists in the form of continuous laments, and this unique advantage contributes to the diversity of silk ber applications. 31,32 The mixing of silk ber and PLA can make up for the problem of poor hydrophilicity of PLA and large brittleness at normal temperature. Another popular protein natural polymer worth mentioning for cartilage repair is collagen.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a novel drug-loaded Bi-layer scaffold for cartilage regeneration

Yue

Lei

et al. 2022

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a novel drug-loaded Bi-layer scaffold for cartilage regeneration

Yue

Lei

et al. 2022

RSC Adv.

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“…[20,21] Silk fibroin (SF), a protein component of silk, has been studied in the field of tissue engineering as a natural polymer with its marked biocompatibility and mechanical properties. [4][5][6][7][20][21][22] SF can easily be physically crosslinked by ultrasound, vortex, pH, and temperature change. [23][24][25] Recently, the possibility of imparting photocurability by mixing SF, having no photocurability, with glycidyl methacrylate (GMA) and substituting a part of the amino acid side chain in SF with a methacrylic acid group has been reported.…”

Section: Introductionmentioning

confidence: 99%

Effect of Silk Fibroin Concentration on the Properties of Polyethylene Glycol Dimethacrylates for Digital Light Processing Printing

et al. 2021

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Attempts are made to use silk fibroin (SF), which is a protein produced by the silkworm, as a scaffold material in the field of regenerative medicine due to its high biocompatibility. Herein, the concentration of SF is varied and mixed with the light‐curing resin polyethylene glycol dimethacrylate (PEGDMA) to create a bioink, and the hydrogel is printed by digital light processing (DLP) 3D printing. Then, to investigate the properties of the printed hydrogel, scanning electron microscope (SEM) observation, swelling behavior measurement, and compression testing are conducted. The properties of hydrogels depend on the concentration of SF, and this result indicates that it is possible to create a scaffold with properties suitable for some tissue by controlling the amount of SF.

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“…After incubation for 48 h, the cell viability of the 50 μg/ml group was slightly higher (* p < 0.05) than those of the 0 and 10 μg/ml groups, and the cell viability of the 100 μg/ml group was the highest (** p < 0.01) ( Figures 3A,B ). We speculate that in a serum-free environment, the amino acids in sericin serve as an energy source for cell metabolism and promote the growth of hPDLSCs ( Lamboni et al, 2015 ; Wu et al, 2021 ). As shown in Figures 3C,D , Live/Dead staining and the CCK-8 assay indicated that Ser-HAP particles did not significantly affect the cell viability of hPDLSCs.…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Ming

Rao

et al. 2022

Front. Bioeng. Biotechnol.

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The guided tissue regeneration (GTR) technique is a promising treatment for periodontal tissue defects. GTR membranes build a mechanical barrier to control the ingrowth of the gingival epithelium and provide appropriate space for the regeneration of periodontal tissues, particularly alveolar bone. However, the existing GTR membranes only serve as barriers and lack the biological activity to induce alveolar bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using a biomimetic mineralization method with sericin as an organic template. The mineralized Ser-HAP showed excellent biocompatibility and promoted the osteogenic differentiation of human periodontal membrane stem cells (hPDLSCs). Ser-HAP was combined with PVA using the freeze/thaw method to form PVA/Ser-HAP membranes. Further studies confirmed that PVA/Ser-HAP membranes do not affect the viability of hPDLSCs. Moreover, alkaline phosphatase (ALP) staining, alizarin red staining (ARS), and RT-qPCR detection revealed that PVA/Ser-HAP membranes induce the osteogenic differentiation of hPDLSCs by activating the expression of osteoblast-related genes, including ALP, Runx2, OCN, and OPN. The unique GTR membrane based on Ser-HAP induces the differentiation of hPDLSCs into osteoblasts without additional inducers, demonstrating the excellent potential for periodontal regeneration therapy.

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Nanocellulose reinforced silkworm silk fibers for application to biodegradable polymers

Cited by 25 publications

References 32 publications

Preparation and characterization of a novel drug-loaded Bi-layer scaffold for cartilage regeneration

Preparation and characterization of a novel drug-loaded Bi-layer scaffold for cartilage regeneration

Effect of Silk Fibroin Concentration on the Properties of Polyethylene Glycol Dimethacrylates for Digital Light Processing Printing

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

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