Nanochannel chromatography and photothermal optical diffraction: Femtoliter sample separation and label-free zeptomole detection

Tsuyama, Yoshiyuki; Morikawa, Kyojiro; Mawatari, Kazuma

doi:10.1016/j.chroma.2020.461265

Cited by 18 publications

(6 citation statements)

References 38 publications

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“…This will greatly contribute to the development of singlecell analysis devices. The developed lysis interface will be combined with our picoliter enzymatic reactor [55], femtoliter isocratic chromatography [56,57], femtoliter gradient chromatography [58], and droplet shooter for a mass spectrometry interface [59] to develop a device for single-cell shotgun proteomics.…”

Section: Discussionmentioning

confidence: 99%

Picoliter liquid handling at gas/liquid interface by surface and geometry control in a micro-nanofluidic device

Morikawa

Murata

Kazoe

et al. 2021

J. Micromech. Microeng.

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In micro- and nanofluidic devices, highly precise fluidic control is essential. Conventional mechanical valves in microchannels and nanochannels have size limitations, whereas hydrophobic (Laplace) valves are generally difficult to use for low-surface-tension liquids. In the present study, we developed a method for handling picoliter volumes of low-surface-tension liquids in a micro-nanofluidic device. The proposed Laplace valve is based on the pinning effect. A fused silica micro-nanofluidic device that includes a picoliter chamber whose geometry was designed to induce capillary pinning was designed and fabricated. The measured Laplace pressure of a lysis buffer (surfactant) was consistent with the calculated pressure, indicating successful fabrication and hydrophobic surface modification. The working principle of the Laplace valve was verified. The Laplace valve maintained the lysis buffer at the gas/liquid interface for 60 min, which is sufficiently long for cell lysis operations. Finally, replacement of liquids in the picoliter chamber using the valve was demonstrated. The proposed method will contribute to basic technologies for fluidic control in micro- and nanofluidic devices, and the proposed Laplace valve can be used for low-surface-tension liquids. In addition, the developed valve and picoliter chamber can be utilized for the interface in single-cell lysis, which will facilitate the development of single-cell analysis devices.

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Section: Discussionmentioning

confidence: 99%

Picoliter liquid handling at gas/liquid interface by surface and geometry control in a micro-nanofluidic device

Morikawa

Murata

Kazoe

et al. 2021

J. Micromech. Microeng.

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“…Even though these values of bonding strength could not tell exact leaking pressure, 600 kPa were high enough to cover practical uses of microfluidic devices. [52][53][54][55] In addition, 6 h of clip-pressing had advantages in low risk of damaging glasses by thermal expansion/ contraction and total length of bonding period. Meanwhile, fusion bonding was overnight process, typically requiring more than 8 h for glass heating, bonding, and cooling in a furnace with risk of thermally damaging glasses.…”

Section: Pressure Resistance Of Glass-glass Bondingmentioning

confidence: 99%

A simple and reversible glass–glass bonding method to construct a microfluidic device and its application for cell recovery

2021

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“…68 However, as the fluidic channel is down to a submicron thickness, which is comparable to the wavelength of light, the TLM signal level is reduced due to the short sample length and quicker heat dissipation, and the diffraction theory of the thermal lens effect needs to be revisited. 69,70 Phase-sensitive detections, such as photothermal optical phase shift (POPS) detection, have been exploited to realize background-free detection and to increase the signal-to-background ratio. POPS was first demonstrated as differential interference contrast thermal lens microscopy (DIC-TLM) for the detection of single metallic nanoparticles in microchannels or capillaries, 71,72 and later further developed for the detection of Sunset Yellow FCF, a nonfluorescent dye in the aqueous solution in nanochannels.…”

Section: Principle Of Photothermal Optical Phase Shiftmentioning

confidence: 99%

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

Chen

Shimizu

Kitamori

2021

J. Optical Microsystems

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Thermal lens microscopy (TLM) utilizes the effects, such as changes in the refractive index, caused by heat generated in the sample for the detection of nonfluorescent analytes with at least a hundred-fold enhancement in sensitivity compared with optical absorbance spectrometry. Micro-/nanofluidic devices can provide specificity and sample manipulation capabilities. The integration of these two technologies exposes the potential to attain the holy grail of continuous, real-time, label-free, specific, and ultrasensitive detection, which find applications in environmental monitoring, quality control of chemical manufacturing, single-cell analysis, and biomedicines. Here, we summarize the recent advances in the instrument development and innovative applications, and suggest future directions of research of TLM. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Nanochannel chromatography and photothermal optical diffraction: Femtoliter sample separation and label-free zeptomole detection

Cited by 18 publications

References 38 publications

Picoliter liquid handling at gas/liquid interface by surface and geometry control in a micro-nanofluidic device

Picoliter liquid handling at gas/liquid interface by surface and geometry control in a micro-nanofluidic device

A simple and reversible glass–glass bonding method to construct a microfluidic device and its application for cell recovery

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

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