Nanoclay-functionalized 3D nanofibrous scaffolds promote bone regeneration

Yao, Qingqing; Fuglsby, Kirby; Zheng, Xiao; Sun, Hongli

doi:10.1039/c9tb02814e

Cited by 35 publications

(25 citation statements)

References 44 publications

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“…Xavier et al [ 27 ] prepared GelMA-XLS hydrogel, improved the mechanical property and degradation behavior of hydrogels and the ALP activity and formation of mineralized nodulus of MC3T3-E1 cells were enhanced in normal culture media with the addition of 2 wt% XLS. Our previous study demonstrated that the degradation products of XLS, i.e., SiO 4 4− , Mg 2+ and Li + combined with the strong binding ability of XLS contributed to the improved osteogenic differentiation [ 49 ] (see Fig. 11 ).…”

Section: Resultsmentioning

confidence: 99%

“…The incorporation of XLS not only increased the stiffness of BG scaffolds, but also enhanced osteogenic differentiation of stem cells . The enhanced osteogenic abilities of XLS were mainly due to two reasons: firstly, the XLS can strongly binding with pro-osteoblastic factors, such as BMPs; secondly, the degradation products from XLS also had positive effects on the osteogenesis of stem cells [ 21 , 49 , 50 ]. Moreover, the elevated HIF-1α and VEGF expressions in ADSCs in vitro suggested the BG-XLS/GelMA-DFO scaffolds had the ability to promote angiogenesis likely through hypoxia mimicking agent activated HIF- 1α signaling pathway [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

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Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

Zheng

Zhang

Wang

et al. 2021

Bioactive Materials

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Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis. In present work, a nanoclay (Laponite, XLS)-functionalized 3D bioglass (BG) scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods. Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells (ADSCs) compared to the properties of the neat BG scaffold. Desferoxamine, a hypoxia mimicking agent, encourages bone regeneration via activating hypoxia-inducible factor-1 alpha (HIF-1α)-mediated angiogenesis. GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation. Furthermore, in vitro data demonstrated increased HIF-1α and vascular endothelial growth factor (VEGF) expressions on human adipose mesenchymal stem cells (ADSCs). Moreover, BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs. Most importantly, our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model. Therefore, we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF, but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

Zheng

Zhang

Wang

et al. 2021

Bioactive Materials

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“…fabricated a Laponite RD‐reinforced 3D gelatin nanofibrous scaffolds via a thermally induced phase separation methodology together with particle leaching. [ 86 ] BMP‐2 was subsequently added to the construct in order to improve bone regeneration ability. In vivo bone regeneration studies demonstrated that those scaffolds with the clay and BMP‐2 resulted in 25.6% new bone area, while the scaffolds without the clay showed only 6.3%.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Despite this, the constructs themselves did not demonstrate sufficient power to create bone tissue alone without the help of BMP‐2. [ 86 ]…”

Section: In Vivo Studiesmentioning

confidence: 99%

Nanoclay Reinforced Biomaterials for Mending Musculoskeletal Tissue Disorders

Erezuma

Eufrásio-da-Silva

Golafshan

et al. 2021

Adv Healthcare Materials

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Nanoclay‐reinforced biomaterials have sparked a new avenue in advanced healthcare materials that can potentially revolutionize treatment of musculoskeletal defects. Native tissues display many important chemical, mechanical, biological, and physical properties that engineered biomaterials need to mimic for optimal tissue integration and regeneration. However, it is time‐consuming and difficult to endow such combinatorial properties on materials via feasible and nontoxic procedures. Fortunately, a number of nanomaterials such as graphene, carbon nanotubes, MXenes, and nanoclays already display a plethora of material properties that can be transferred to biomaterials through a simple incorporation procedure. In this direction, the members of the nanoclay family are easy to functionalize chemically, they can significantly reinforce the mechanical performance of biomaterials, and can provide bioactive properties by ionic dissolution products to upregulate cartilage and bone tissue formation. For this reason, nanoclays can become a key component for future orthopedic biomaterials. In this review, we specifically focus on the rapidly decreasing gap between clinic and laboratory by highlighting their application in a number of promising in vivo studies.

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“…Bone marrow mesenchymal stem cells (BMSCs) is a process comprised of multilineage differentiation capacities, including osteogenesis and chondrogenesis, which has garnered increasing attention over the past decades (Charbord, 2010). Recent experimental studies have revealed that modified mesenchymal stem cells (MSCs) enhance osteogenic commitment and exhibit notable therapeutic implications to promote bone formation and regeneration (Wu, Wang, Wang, Liang, & Li, 2020; Yao, Fuglsby, Zheng, & Sun, 2020). Several factors including bone morphogenetic protein‐2 (Ramazzotti et al, 2016), and runt‐related transcription factor 2 (RUNX2; J. M. Kim et al, 2020), parathyroid hormone (Yang et al, 2019), have been implicated in the process of osteogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

miR‐101‐loaded exosomes secreted by bone marrow mesenchymal stem cells requires the FBXW7/HIF1α/FOXP3 axis, facilitating osteogenic differentiation

Wang

et al. 2021

Journal Cellular Physiology

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Exosomes derived from mesenchymal stem cells (MSCs) have emerged as significant mediators of intercellular communication, with studies highlighting their role in the transmission of biological signals between cells. Dominant microRNA (miRNA)-mediated translational repression of messenger RNAs has been extensively investigated in regard to its influence in orchestrating osteogenic differentiation. In the current study, we sought to ascertain the contributory role of miRNA-101 (miR-101) encapsulated in the process of bone marrow mesenchymal stem cell (BMSC)-derived exosomes in osteogenic differentiation. Exosomes were initially extracted from BMSCs at Days 0, 3, 12, and 21 of osteogenic differentiation by ultracentrifugation. Artificial modulation of miR-101 and FBXW7 (silencing and overexpression) were performed in the BMSCs to identify its effects on osteogenic factors, alkaline phosphatase activity, and osteogenic differentiation. Mechanistic exploration was performed to evaluate the binding affinity between miR-101 and FBXW7, the FBXW7-mediated HIF1α ubiquitination, and the HIF1α enrichment in the FOXP3 promoter region. Exosomes from MSCs in the late stage of osteogenic differentiation exhibited enhanced osteogenic differentiation. Upregulated miR-101 in MSC-derived exosomes was detected during osteogenic differentiation, while diminished levels of FBXW7 expression was noted. Importantly, miR-101 was found to specifically bind to the 3′-untranslated region of FBXW7. Meanwhile, data was obtained indicating that FBXW7 could ubiquitinate and degrade HIF1α to repress its upregulation during osteogenic differentiation. HIF1α bound to the promoter region of FOXP3 to facilitate osteogenic differentiation. Ultimately, the findings of the current study demonstrate that BMSC-derived exosomal miR-101 augments osteogenic differentiation in MSCs by inhibiting FBXW7 to regulate the HIF1α/FOXP3 axis.

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Nanoclay-functionalized 3D nanofibrous scaffolds promote bone regeneration

Abstract: A nanoclay-enriched 3D nanofibrous biomimetic scaffold with strong bone regenerative ability by virtue of its improved mechanical properties, osteoconductivity, and drug binding capacity was developed.

Cited by 35 publications

References 44 publications

Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

Nanoclay Reinforced Biomaterials for Mending Musculoskeletal Tissue Disorders

miR‐101‐loaded exosomes secreted by bone marrow mesenchymal stem cells requires the FBXW7/HIF1α/FOXP3 axis, facilitating osteogenic differentiation

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