Nanoclay mineral-reinforced macroporous nanocomposite scaffolds for in situ bone regeneration: In vitro and in vivo studies

Wu, Minhao; Chen, Feixiang; Wu, Ping; Yang, Zhiqiang; Zhang, Sheng; Xiao, Lingfei; Deng, Zhouming; Zhang, Chong; Chen, Yun; Cai, Lin

doi:10.1016/j.matdes.2021.109734

Cited by 44 publications

(29 citation statements)

References 53 publications

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“…2a . Under low magnification, all scaffolds were characterized by a typical sponge-like structure consisting of highly interconnected macropores throughout the scaffolds, as demonstrated in our previous study 31 . These 3D porous and interconnected structures of scaffolds are important structural traits that significantly promote the ingrowth of new bone tissue, vascularization, and transportation of nutrients and waste material, thereby accelerating bone defect repair and regeneration 32 .…”

Section: Resultssupporting

confidence: 71%

“…MC3T3-E1 cells and HUVECs were maintained in DMEM containing 10% FBS and 1% P/S under 5% CO 2 and a humidified atmosphere of 37 °C. In addition to MC3T3-E1 cells, rBMSCs were further isolated to assess cell adhesion and osteogenic differentiation according to our established protocol 31 . Cells were passaged using 0.25% trypsin-EDTA when they reached 80–90% confluency.…”

Section: Methodsmentioning

confidence: 99%

“…To further determine the cytocompatibility of the fabricated scaffolds, cells (1 × 10 5 cells/mL) were cultured on the scaffolds for 3 days. After that, live/dead staining was performed according to our established protocol 31 . The fluorescent images were observed using CLSM (TCS SP8, Leica, Germany) with 488 nm (green, calcein-AM) and 561 nm (red, EthD-1) excitation filters.…”

Section: Methodsmentioning

confidence: 99%

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Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

Zhang

et al. 2022

npj Regen Med

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Osteogenesis and osteoclastogenesis are closely associated during the bone regeneration process. The development of multifunctional bone repair scaffolds with dual therapeutic actions (pro-osteogenesis and anti-osteoclastogenesis) is still a challenging task for bone tissue engineering applications. Herein, through a facile surface coating process, mussel-inspired polydopamine (PDA) is adhered to the surface of a biocompatible porous scaffold followed by the immobilization of a small-molecule activator (LYN-1604 (LYN)) and the subsequent in situ coprecipitation of hydroxyapatite (HA) nanocrystals. PDA, acting as an intermediate bridge, can provide strong LYN immobilization and biomineralization ability, while LYN targets osteoclast precursor cells to inhibit osteoclastic differentiation and functional activity, which endows LYN/HA-coated hybrid scaffolds with robust anti-osteoclastogenesis ability. Due to the synergistic effects of the LYN and HA components, the obtained three-dimensional hybrid scaffolds exhibited the dual effects of osteoclastic inhibition and osteogenic stimulation, thereby promoting bone tissue repair. Systematic characterization experiments confirmed the successful fabrication of LYN/HA-coated hybrid scaffolds, which exhibited an interconnected porous structure with nanoroughened surface topography, favorable hydrophilicity, and improved mechanical properties, as well as the sustained sequential release of LYN and Ca ions. In vitro experiments demonstrated that LYN/HA-coated hybrid scaffolds possessed satisfactory cytocompatibility, effectively promoting cell adhesion, spreading, proliferation, alkaline phosphatase activity, matrix mineralization, and osteogenesis-related gene and protein secretion, as well as stimulating angiogenic differentiation of endothelial cells. In addition to osteogenesis, the engineered scaffolds also significantly reduced osteoclastogenesis, such as tartrate-resistant acid phosphatase activity, F-actin ring staining, and osteoclastogenesis-related gene and protein secretion. More importantly, in a rat calvarial defect model, the newly developed hybrid scaffolds significantly promoted bone repair and regeneration. Microcomputed tomography, histological, and immunohistochemical analyses all revealed that the LYN/HA-coated hybrid scaffolds possessed not only reliable biosafety but also excellent osteogenesis-inducing and osteoclastogenesis-inhibiting effects, resulting in faster and higher-quality bone tissue regeneration. Taken together, this study offers a powerful and promising strategy to construct multifunctional nanocomposite scaffolds by promoting osteo/angiogenesis and suppressing osteoclastogenesis to accelerate bone regeneration.

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Section: Resultssupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

Zhang

et al. 2022

npj Regen Med

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“…The ethanol displacement method was used to determine the porosity of the FVC, as previously described. 41 2.2.2. Mechanical Properties' Testing.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the cross-sectional images of the FVC were used to determine the average pore size (≥200 samples) by ImageJ software (NIH, Bethesda, MD). The ethanol displacement method was used to determine the porosity of the FVC, as previously described …”

Section: Methodsmentioning

confidence: 99%

Natural Flammulina velutipes-Based Nerve Guidance Conduit as a Potential Biomaterial for Peripheral Nerve Regeneration: In Vitro and In Vivo Studies

Chen

et al. 2021

ACS Biomater. Sci. Eng.

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The treatment and repair of serious peripheral nerve injuries remain challenging in the clinical practice, while the application of multifunctional nerve guidance conduits (NGCs) based on naturally derived polymers has attracted much attention in recent years because of their excellent physicochemical properties and biological characteristics. Flammulina velutipes (Curt. ex FV) is a popular edible mushroom characterized by hollow tubular structures, antibacterial activities, and high nutritional properties. In this study, FV is utilized to construct NGCs (labeled FVC) via a freeze-drying technique without chemical modifications. The morphology, physical properties, cellular biocompatibility, antibacterial properties, and nerve regeneration capacity of FVC were assessed both in vitro and in vivo. FVC is composed of hollow tubes and evenly irregular interconnected micropores with 73.8 ± 5.5% porosity and 476.1 ± 12.9 μm hollow tube diameter. The inner surface of the FVC presents multiple microgrooves elongated parallel to the long axis. Moreover, FVC possessed strong antibacterial activity and could inhibit Gram-positive Staphylococcus aureus growth by up to 96.0% and Gram-negative Escherichia coli growth by up to 94.8% in vitro. FVC exhibited excellent biocompatibility and effectively promoted PC-12 cell proliferation and elongation in vitro. When applied to repair critical-sized sciatic nerve defects, FVC could effectively stimulate nerve functional recovery and axonal outgrowth in a rat model. Interestingly, Western blot analysis indicated that growth-associated protein 43 (GAP-43) had increased expression levels in the FVC group compared with the autograft group. This result suggested that by activating the Janus activated kinase2 (JAK2)/Phosphorylation ofsignal transducer and activator of transcription-3 (STAT3) signaling pathway, FVC upregulated Phosphorylation of signal transducer and activator of transcription-3 (P-STAT3) in vivo, resulting in the secretion of GAP-43. Collectively, a natural NGC FVC was fabricated based on FV without chemical modifications. The morphology, physical properties, cellular biocompatibility, antibacterial properties, and nerve regeneration capacity of FVC provide new insights for its further optimization and application in the field of nerve tissue engineering.

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Medical Geology

Prasad¹

2023

Medical Geology

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Nanoclay mineral-reinforced macroporous nanocomposite scaffolds for in situ bone regeneration: In vitro and in vivo studies

Cited by 44 publications

References 53 publications

Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration

Natural Flammulina velutipes-Based Nerve Guidance Conduit as a Potential Biomaterial for Peripheral Nerve Regeneration: In Vitro and In Vivo Studies

Medical Geology

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