Nanocurcumin–pyrroloquinoline formulation prevents hypertrophy–induced pathological damage by relieving mitochondrial stress in cardiomyocytes under hypoxic conditions

Abstract

This study investigates the therapeutic effect of a nanocurcumin formulation (NCF) containing nanocurcumin (NC) and pyrroloquinoline quinone (PQQ) on ameliorating hypoxia-induced stress in hypertrophied primary human ventricular cardiomyocytes (HVCM) under hypoxic conditions, as validated in a Sprague-Dawley rat model of chronic hypobaric hypoxia (cHH)-induced right ventricular hypertrophy (RVH). Based on our previous findings, here, we analyzed the improvement in the protective efficacy of NCF against mitochondrial damage. The electron transport chain Complexes’ activities were analyzed as a chief operational center for mitochondrial homeostasis, along with key gene and protein markers for mitochondrial biogenesis, redox function, fatty acid oxidation, bio-energetic deficit and cell survival. NCF supplementation imparts cyto-protection from hypoxia-induced hypertrophy and damage in both in vitro and in vivo models while maintaining mitochondrial homeostasis better than NC and PQQ alone. This study proposes the use of NCF as a potential candidate molecule for imparting protection from high altitude-induced maladies in ascendants.

Pyrroloquinoline quinone attenuates isoproterenol hydrochloride‑induced cardiac hypertrophy in AC16 cells by inhibiting the NF‑κB signaling pathway

“…The above-mentioned results indicate that the effects of PQQ on protecting the heart from ischemia/reperfusion injury may be accomplished by its ability to scavenge free radicals to protect the mitochondria from oxidative stress. In addition, it has been reported that the nanocurcumin-PQQ formulation prevents hypertrophy-induced pathological damage by relieving mitochondrial stress in cardiomyocytes under hypoxic conditions, while under these conditions, PQQ treatment alone can improve cellular viability (19).…”

“…A previous study demonstrated that PQQ can modulate the number and function of the mitochondria in mice, and can directly react with reactive oxygen species (ROS) in the mitochondria to maintain the dynamic balance of ROS (16). However, to date, at least to the best of our knowledge, there are only few reports on the effects of PQQ on cardiac hypertrophy (17)(18)(19)(20)(21). Based on previous studies (17)(18)(19)(20)(21), it was hypothesized that PQQ may be protective against Iso-induced cardiac hypertrophy.…”

“…However, to date, at least to the best of our knowledge, there are only few reports on the effects of PQQ on cardiac hypertrophy (17)(18)(19)(20)(21). Based on previous studies (17)(18)(19)(20)(21), it was hypothesized that PQQ may be protective against Iso-induced cardiac hypertrophy. There are numerous studies on the pathogenesis of cardiac hypertrophy focusing on myocardial pressure overload, myocardial apoptosis, vascular remodeling, oxidative stress and the inflammatory response (4,(22)(23)(24)(25)(26); however, the pathogenesis of cardiac hypertrophy has not yet been fully clarified.…”

Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats

“…On a global scale, various alternative strategies are evaluated for cardio‐protective attributes. It is in general consensus that employment of natural bioactive materials promotes homeostasis and/or protection under stress/challenge with minimal untoward effects . In this regards, traditional Chinese medicines shares a long history of high repute and may plausibly lay the foundation for better therapeutic interventions for cardiovascular ailments including hypertrophy .…”

Applications of Nanocarriers as Drug Delivery Vehicles for Active Phytoconstituents

“…The same authors then used formulations combined with nanocurcumin and pyrroloquinoline, a polyphenolic compound found in kiwi fruits, to study their effect against mitochondrial damage. The formulation showed a higher potency in modulating cardiac hypertrophy than nanocurcumin or pyrroloquinoline alone [113]. Recently, an in-vivo study showed magnetic hydrogel nanocomposite loaded with curcumin that was able to show a cardioprotective effect against toxicity induced by doxorubicin.…”