Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol

Ронжин, Н. О.; Барон, А. В.; Mamaeva, E. S.; Puzyr, A. P.; Bondar, V. S.

doi:10.4236/jbnb.2013.43030

Cited by 9 publications

(7 citation statements)

References 14 publications

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Section: Research Articlementioning

confidence: 99%

“…In our previous studies, we showed that MNDs can be used to construct systems for biochemical diagnostics and targeted delivery of drugs and new antibacterial preparations [29][30][31]. The MND-enzyme systems were used in biochemical assays of physiologically significant analytes [29]. The immobilization of the sensing element (nanoparticle-biomarker conjugate) on a solid support can be adapted and advanced the experimental indicator system for clinical analytical applications.…”

Section: Research Articlementioning

confidence: 99%

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A Supramolecular Indicator System for Detecting Urea Based on the Cellulose-Nanodiamond Composite Material and Urease

Ekaterina¹,

Nikita²,

Olga³

et al. 2019

Int J Med Nano Res

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A supramolecular indicator system (cellulose-nanodiamonds-urease) for reusable biochemical detection of urea has been fabricated using sequential immobilization of the components. Modified nanodiamonds (MNDs) were covalently immobilized onto DEAE cellulose granules via the nucleophilic addition reaction. At DEAE cellulose: MND ratio of 4:1 (w/w), up to 110 µg of nanoparticles bound onto 1 mg of the polymer during the addition reaction. Urease was immobilized by covalent conjugation onto the polymer-MND composite with the benzoquinone-activated surface. In comparative experiments, the enzyme was immobilized onto initial polymer granules via nonspecific adsorption and covalent conjugation. However, when these indicator systems were repeatedly used to detect the analyte, the enzyme was considerably inactivated, and that was evidenced by a decrease in the colored product yield. At the same time, the enzyme covalently bound onto the DEAE cellulose-MND composite showed higher functional efficacy and enabled more stable yields of the colored product in repeated urea assays. Comparative experiments with the indicator systems repeatedly used to detect urea at 37 °C demonstrated that the enzyme covalently conjugated onto the DEAE cellulose-MND composite showed greater thermo stability, and its activity was reduced at a much slower rate than the activity of the enzyme covalently bound to the polymer. The data obtained in the present study offer the prospect of designing a new type of reusable indicator assay systems (polymer carrier-nanodiamond-biomarker supramolecular systems) for biomedical analytical applications.

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Section: Research Articlementioning

confidence: 99%

Section: Research Articlementioning

confidence: 99%

A Supramolecular Indicator System for Detecting Urea Based on the Cellulose-Nanodiamond Composite Material and Urease

Ekaterina¹,

Nikita²,

Olga³

et al. 2019

Int J Med Nano Res

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“…This opens prospects for the application of these nanoparticles for the separation and purification of biomolecules [13] [14] [15], immobilization and neutralization of toxins (e.g. mycotoxins) [16] [17], and creation of new systems for biochemical diagnosis [18] and new drug delivery systems [19].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Nanodiamonds at the Action of Colored Metal Ions on the Skin of Guinea Pigs

Vasilyeva¹,

Prokhorenkov²,

Puzyr³

et al. 2016

JBNB

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The protective effect of modified nanodiamonds (MND) under the action of cobalt and nickel ions on the skin of Guinea pigs was shown. At the action of chromium ions on the skin of animals, the protective effect of MND was not found. The differences are related to different adsorption properties of MND to the investigated colored metal ions. It is shown in vitro that MND can adsorb ions of cobalt and nickel and don't bind ions of chromium from aqueous medium. The perspectives using of MND as a new drug for the prevention allergic dermatitis caused by action of bivalent ions of metals are discussed.

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“…Earlier, we demonstrated the applicability of mod ified nanodiamonds (MNDs) obtained by detonation synthesis for separation and purification of proteins [1,2], design of systems for targeted delivery of biolog ically active compounds [3], and biochemical diagno sis of physiologically important compounds [4,5]. Further in vitro studies have shown the possibility of adsorption on MND particles of a number of protein and nonprotein components of human blood serum [6].…”

mentioning

confidence: 99%

Binding the immunoglobulins of human serum by nanodiamonds

Барон

Osipov

Olkhovskiy

et al. 2014

Dokl Biochem Biophys

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Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol

Cited by 9 publications

References 14 publications

A Supramolecular Indicator System for Detecting Urea Based on the Cellulose-Nanodiamond Composite Material and Urease

A Supramolecular Indicator System for Detecting Urea Based on the Cellulose-Nanodiamond Composite Material and Urease

The Effects of Nanodiamonds at the Action of Colored Metal Ions on the Skin of Guinea Pigs

Binding the immunoglobulins of human serum by nanodiamonds

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