Nanoencapsulation of Chromolaena odorata Extract Using Pluronic F127 as an Effectively Herbal Delivery System for Wound Healing

Abstract

Chromolaena odorata is a medicinal herb with prominent pharmacological properties. The therapeutic efficiency of Chromolaena odorata extracts and its ingredients have, however, been limited by various factors, including the lack of targeting capacity and poor bioavailability. To approach this drawback, ethyl acetate fraction extract of Chromolaena odorata- (EA.ChO-) encapsulated pluronic-based nanocarriers was disclosed herein. The most common pluronic triblock copolymer micelles (pluronic F127) was used for the nanosized formulation of Chromolaena odorata extract. The obtained results show that EA.ChO-encapsulated nanoparticles have a spherical morphology with a designed hydrodynamic size was about 183.7 nm and zeta potential -39.5 mV. The EA.ChO nanoparticles are stable in different aqueous solutions (water, PBS 2.8, and PBS 7.4). The lyophilized form of the EA.ChO nanoparticles exhibited excellent stability for long-term storage. Notably, the EA.ChO nanoparticles were 1.3-1.4 fold more effective in the growth of fibroblast than the free EA.ChO, verifying the potential of pluronic F127 nanoparticles to the increased function of EA.ChO in the proliferation of fibroblast cell. In addition, bleeding stopped within 55 ± 6 s which was 20 s faster than that of free EA.ChO and 38-44 s faster than that of negative control treatments. The EA.ChO nanoencapsulation processed a rapid blood clot formation compared to control, free EA.ChO, pluronic F127, and water, suggesting the excellent bioavailability of EA.ChO nanoencapsulation. The obtained results thus provided a promising prospect for raising the activity Chromolaena odorata extract in wound healing application.

Pluronic® F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation

“…The hydrogel dispersed in water generated micelles with a size of 7.55 nm (100% of the micelles population) of hydrodynamic diameter (h.d) and a polydispersity index (PdI) of 0.223. The literature reports diameters of 10.6 nm [52] for Pluronic ® F127 water solutions, similar to the value found in our analysis. Additionally, hydrogel without extract (Figure 1E) showed similar micelles characteristics, in which 100% of the micelles population presented a size of 7.24 nm and PdI of 0.321 values.…”

“…However, at 1000 µg/mL, the cell viability was similar to the control. This interesting effect of VADE-loaded-hydrogel on fibroblast proliferation (Figure 7B) was also detected by Luu et al [52] using Pluronic ® F127 in the nanoencapsulation of Chromolaena odorata extracts. The concentration-dependent cytotoxicity was based on Pluronic ® F127 micelles and, as detected by our results, presented no damage on human fibroblast cells.…”