Nanofiber technology in the ex vivo expansion of cord blood-derived hematopoietic stem cells

Ferreira, Mónica S. Ventura; Mousavi, Seyed Hadi

doi:10.1016/j.nano.2018.04.017

Cited by 20 publications

(13 citation statements)

References 91 publications

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“…Polycaprolactone (PCL) is a biocompatible, biodegradable and nontoxic linear aliphatic polyester and it can be easily fabricated into thin membranes while maintaining its mechanical strength. Because of the characteristics described above, PCL has been used in various applications, including scaffolds, nanoparticles, nanofibers, composites, hydrogels and films for TE [14].…”

Section: Discussionmentioning

confidence: 99%

“…The different chemistries of the scaffold, scaffold geometry (porosities, fiber diameters, and orientation), physicochemical parameters (hydrophobicity, degradation time or protein adsorption) have an influence on HSC expansion. Also, culture condition such as contact with stromal cells or ECM, the number of initiation of CD34+ cell culture, oxygen concentration, shear stress, duration of culture, cytokine cocktail, and cell culture medium has an impact on expansion and maintenance of HSC [14].…”

Section: Discussionmentioning

confidence: 99%

“…Our electrospun fiber scaffolds are their microscale construction with greater surface-to-volume ratio and capacity to selectively enhance absorption of ECM proteins that are able to regulate communications of the cells with the microenvironment and cell interaction [14]. In 2D cell culture system, this communication capacity is low and restricted to neighboring cells, therefore, TNC and CD34+ cell expansion in 2D is lower than 3D.…”

Section: Enhanced Colony Formation Of the Cells Expanded In The Pcl-cmentioning

confidence: 99%

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Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Hadi¹,

Abroun²,

Soleimani³

et al. 2019

Int J Stem Cell Res Ther

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Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen. Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity. Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher total nucleated cells (50-fold vs. 38-fold) and CD34+ cells (20-fold vs. 2.6-fold) (p < 0.05) and compared to 2-Dimensional cell culture and before expansion had higher expression of homing and self-renewal genes and for VLA-4, hTERT and BMI-1 genes were statically significant (p = 0.0001). The expression of myeloid markers in 3-Dimensional scaffold was significantly higher than the 2-Dimensional culture system (p < 0.05). The total colony in 2-Dimensional culture was lower than 3-Dimensional culture medium (p < 0.05). Conclusion: This study demonstrated the synergistic effect between the three-dimensionality of the scaffold and collagen as an extracellular matrix protein, and the potential of this 3-Dimensional Polycaprolactone nanofiber scaffold coated with collagen for ex vivo expansion of HSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Enhanced Colony Formation Of the Cells Expanded In The Pcl-cmentioning

confidence: 99%

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Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Hadi¹,

Abroun²,

Soleimani³

et al. 2019

Int J Stem Cell Res Ther

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“…In the endosteal niche, HSC prevalently reside in a quiescent state due to high concentrations of Ca 2+ and hypoxia. The second subniche is termed the vascular niche ( Figure 2A ) where, by contrast, HSC can easily proliferate and differentiate because there is a higher level of oxygen and lower concentration of Ca 2+ in comparison with the endosteal niche ( Ferreira and Mousavi, 2018 ). Niche physical properties strongly influence HSC fate.…”

Section: Hematopoietic Stem Cells and Hematopoiesismentioning

confidence: 99%

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Zmrhal

Svoradová

Bátik

et al. 2022

Front. Cell Dev. Biol.

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Three-dimensional (3D) cell culture is attracting increasing attention today because it can mimic tissue environments and provide more realistic results than do conventional cell cultures. On the other hand, very little attention has been given to using 3D cell cultures in the field of avian cell biology. Although mimicking the bone marrow niche is a classic challenge of mammalian stem cell research, experiments have never been conducted in poultry on preparing in vitro the bone marrow niche. It is well known, however, that all diseases cause immunosuppression and target immune cells and their development. Hematopoietic stem cells (HSC) reside in the bone marrow and constitute a source for immune cells of lymphoid and myeloid origins. Disease prevention and control in poultry are facing new challenges, such as greater use of alternative breeding systems and expanding production of eggs and chicken meat in developing countries. Moreover, the COVID-19 pandemic will draw greater attention to the importance of disease management in poultry because poultry constitutes a rich source of zoonotic diseases. For these reasons, and because they will lead to a better understanding of disease pathogenesis, in vivo HSC niches for studying disease pathogenesis can be valuable tools for developing more effective disease prevention, diagnosis, and control. The main goal of this review is to summarize knowledge about avian hematopoietic cells, HSC niches, avian immunosuppressive diseases, and isolation of HSC, and the main part of the review is dedicated to using 3D cell cultures and their possible use for studying disease pathogenesis with practical examples. Therefore, this review can serve as a practical guide to support further preparation of 3D avian HSC niches to study the pathogenesis of avian diseases.

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“…Manufactured nanofibers have high stability in vivo, and their material properties are easy to optimize according to the cell type, study purpose, cell adhesion, infiltration, and porosity, which governs the transport of nutrients and oxygen [111]. On account of their mechanical, thermal, and chemical characteristics, synthetic polymers such as polyethylene terephthalate (PET), polyurethane (PU), and polyethersulfone (PES) have been employed for BM niche mimicry [112]. Although synthetic polymers intrinsically lack well-known cell-binding sites to localize signals, their exceptional flexibility in synthesis allows straightforward modification to provide such sites.…”

Section: Engineering Hsc Niches For Modulating Cell Behavior In Vitromentioning

confidence: 99%

The physical microenvironment of hematopoietic stem cells and its emerging roles in engineering applications

Zhang

et al. 2019

Stem Cell Res Ther

115

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Stem cells are considered the fundamental underpinnings of tissue biology. The stem cell microenvironment provides factors and elements that play significant roles in controlling the cell fate direction. The bone marrow is an important environment for functional hematopoietic stem cells in adults. Remarkable progress has been achieved in the area of hematopoietic stem cell fate modulation based on the recognition of biochemical factors provided by bone marrow niches. In this review, we focus on emerging evidence that hematopoietic stem cell fate is altered in response to a variety of microenvironmental physical cues, such as geometric properties, matrix stiffness, and mechanical forces. Based on knowledge of these biophysical cues, recent developments in harnessing hematopoietic stem cell niches ex vivo are also discussed. A comprehensive understanding of cell microenvironments helps provide mechanistic insights into pathophysiological mechanisms and underlies biomaterial-based hematopoietic stem cell engineering.

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Nanofiber technology in the ex vivo expansion of cord blood-derived hematopoietic stem cells

Cited by 20 publications

References 91 publications

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

The physical microenvironment of hematopoietic stem cells and its emerging roles in engineering applications

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