Nanofibrous scaffold prepared by electrospinning of poly(vinyl alcohol)/gelatin aqueous solutions

Abstract

A series of nanofibrous scaffolds were prepared by electrospinning of poly(vinyl alcohol) (PVA)/gelatin aqueous solution. PVA and gelatin was dissolved in pure water and blended in full range, then being electrospun to prepared nanofibers, followed by being crosslinked with glutaraldehyde vapor and heat treatment to form nanofibrous scaffold. Field emission scanning electron microscope (FESEM) images of the nanofibers manifested that the fiber average diameters decreased from 290 to 90 nm with the increasing of gelatin. In vitro degradation rates of the nanofibers were also correlated with the composition and physical properties of electrospinning solutions. Cytocompatibility of the scaffolds was evaluated by cells morphology and MTT assay. The FESEM images revealed that NIH 3T3 fibroblasts spread and elongated actively on the scaffolds with spindle-like and star-type shape. The results of cell attachment and proliferation on the nanofibrous scaffolds suggested that the cytotoxicity of all samples are grade 1 or grade 0, indicating that the material had sound biosafety as biomaterials. Compared with pure PVA and gelatin scaffolds, the hybrid ones possess improved biocompatibility and controllability. These results indicate that the PVA/gelatin nanofibrous have potential as skin scaffolds or wound dressing.

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

“…It is affected by the material's hydrophilicity/hydrophobicity, nanotopography, mechanical properties, and functional groups on nanofiber surface Pelipenko et al, 2014;Yang et al, 2011). Reports on the effects of nanofibrillar topography on the rate of cell adhesion are contradictory.…”

Core‐shell nanofibers: Integrating the bioactivity of gelatin and the mechanical property of polyvinyl alcohol

“…In the electrospinning of composite nanofibers, gelatin is frequently blended with synthetic polymer . Although the composite nanofibers display an enhanced biocompatibility over their counterparts composed solely of synthetic polymers, the electrospinning of the blended solution tends to create beaded nanofibers, attributing to the low cell adhesion . Here, coaxial‐electrospinning is used to fabricate PVA and gelatin into defect‐free composite nanofibers with PVA in the core and gelatin in the shell.…”

Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration

“…Later, Yang et al . evaluated the biocompatibility of PVA-gelatin nanofibers16. Due to the non-adhesive property but biocompatibility of PVA which limit the adhesion and spreading of cells, the comparative study of PVA and gelatin-incorporated PVA nanofibers can explore the topography-induced biological effects.…”