Nanogels based on alginic aldehyde and gelatin by inverse miniemulsion technique: synthesis and characterization

Sarika, P.R.; Kumar, P.R. Anil; Raj, Deepa K.; James, Nirmala Rachel

doi:10.1016/j.carbpol.2014.11.037

Cited by 76 publications

(28 citation statements)

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“…Regarding the GCR‐GLU film, the aldehyde groups of the crosslinking agent can react with amino groups of the gelatin to form C=N groups (Schiff's base). The absorption peak of this group is usually around 1620‐1660 cm ‐1 , which might have been masked by stronger Amide‐I bands.…”

Section: Resultsmentioning

confidence: 99%

Gelatin capsule residue‐based films crosslinked with the natural agent genipin

et al. 2019

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This study focused on the development and characterization of biodegradable films based on gelatin capsule residue crosslinked with the natural agent genipin extracted from genipap (Genipa americana L.). Their potential on maintaining the quality attributes (level of peroxides, conjugated dienes and trienes and color) of extra‐virgin olive oil under accelerated storage conditions was investigated. Results showed that the genipin‐crosslinked films had a dark blue appearance. Their total soluble matter, water vapor permeability, tensile strength, ultraviolet and visible light barriers and thermal properties were improved in relation to the uncrosslinked film, whereas their moisture content and microstructure were not affected. In general, films presented comparable properties to those of glutaraldehyde‐crosslinked films. The developed films successfully protected the packed oil from primary and secondary oxidation and maintained the color over the storage period. Therefore, this study demonstrated the effectiveness of genipin on improving the properties of gelatin‐based films and the promising application of films as food packaging.

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Section: Resultsmentioning

confidence: 99%

Gelatin capsule residue‐based films crosslinked with the natural agent genipin

et al. 2019

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“…It is well known that large amounts of EVs are normally present in FBS [ 46 ]. Therefore, great attention must be paid to assure that no contaminants are introduced by the use of culture media and supplements during the cell expansion phase.…”

Section: Discussionmentioning

confidence: 99%

Pilot Production of Mesenchymal Stem/Stromal Freeze-Dried Secretome for Cell-Free Regenerative Nanomedicine: A Validated GMP-Compliant Process

Bari

Perteghella

Silvestre

et al. 2018

Cells

128

163

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In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in a validated good manufacturing practice (GMP)-compliant cell factory. Secretome was purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained a freeze-dried, “ready-off-the-shelf” and free soluble powder containing extracellular vesicles and proteins. In the freeze-dried product, a not-aggregated population of extracellular vesicles was detected by nanoparticle tracking analysis; Fourier transform infrared spectra showed the simultaneous presence of protein and lipids, while differential scanning calorimetry demonstrated that lyophilization process successfully occurred. A proteomic characterization allowed the identification of proteins involved in immune response, response to stress, cytoskeleton and metabolism. Moreover, the product was not cytotoxic up to concentrations of 25 mg/mL (on human fibroblasts, chondrocytes and nucleus pulposus cells by MTT assay) and was blood compatible up to 150 mg/mL. Finally, at concentrations between 5 and 50 mg/mL, freeze-dried secretome showed to in vitro counteract the oxidative stress damage induced by H2O2 on nucleus pulposus cells by MTT assay.

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“…One commonly applied strategy aims at the formation of (semi-)interpenetrating networks (IPNs) by cross-linking the gelatin independently from the other polymer [16,17]. Alternatively, chemically oxidized alginate has already been applied of which the aldehyde functionalities readily bind to the free amines of gelatin [18][19][20]. The current work can be situated at the interface of both strategies.…”

Section: Materials Synthesismentioning

confidence: 95%

Cross-linkable alginate-graft-gelatin copolymers for tissue engineering applications

Graulus

Mignon

Vlierberghe

et al. 2015

European Polymer Journal

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a b s t r a c tWhen it comes to failing or injured tissues and organs, patients often end up on waiting lists for tissue or even organ transplantation negatively affecting the patient's quality of life. The multidisciplinary research field of tissue engineering may offer more innovative ways to replace or ideally regenerate failing tissues and organs. A widely used material in this research field is gelatin because of its biocompatibility and interesting hydrogel forming properties. However, at body temperature gelatin's mechanical properties are greatly reduced due to the dissolution of collagen-like triple helices. With the aim to obtain materials that retain their mechanical properties at body temperature, we propose to combine sodium alginate and methacrylamide-modified gelatin (Gel-MOD) in the form of a graft copolymer to obtain a material that closely resembles the extracellular matrix. The obtained materials can be cross-linked via three distinct pathways including cation mediated, temperature mediated or via covalent bond formation after UV irradiation in the presence of a photo-initiator. The current contribution covers the synthesis of the above mentioned alginate-graft-gelatin copolymers and the characterization of the resulting hydrogels. The materials developed are highly hydrophilic, showing high gel fractions and satisfactory mechanical properties. Moreover the attained storage moduli were tunable by divalent cation addition (72-275% increase at 21°C, 42-405% increase at 40°C). One formulation was found to outperform Gel-MOD in terms of mechanical properties at 40°C, thus indicating the proposed strategy can be used to improve the mechanical properties of gelatin-based hydrogels. Moreover, in vitro biocompatibility assays indicated that cell adhesion and proliferation improves with increasing gelatin content. The present paper illustrates that the developed triple cross-linkable materials are suitable cell carriers, promising to be applied for biomedical purposes.

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Nanogels based on alginic aldehyde and gelatin by inverse miniemulsion technique: synthesis and characterization

Cited by 76 publications

References 50 publications

Gelatin capsule residue‐based films crosslinked with the natural agent genipin

Gelatin capsule residue‐based films crosslinked with the natural agent genipin

Pilot Production of Mesenchymal Stem/Stromal Freeze-Dried Secretome for Cell-Free Regenerative Nanomedicine: A Validated GMP-Compliant Process

Cross-linkable alginate-graft-gelatin copolymers for tissue engineering applications

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