Nanogels of Natural Polymers

Pamfil, Daniela; Vasile, Cornelia

doi:10.1007/978-981-10-6080-9_4

Cited by 14 publications

(12 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gelatin has been used for nanogels preparation for different biomedical applications [401]. In this respect, activated PEG methyl ether was grafted onto gelatin backbone to obtain the gelatin-PEG copolymer and then self-assembled to form nanogels (Figure 8) that were encapsulated with poor water soluble curcumin (CUR) for cancer treatment [402].…”

Section: Drug and Molecule Deliverymentioning

confidence: 99%

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Vasile¹,

Pamfil²,

Stoleru³

et al. 2020

Molecules

Self Cite

210

131

View full text Add to dashboard Cite

New trends in biomedical applications of the hybrid polymeric hydrogels, obtained by combining natural polymers with synthetic ones, have been reviewed. Homopolysaccharides, heteropolysaccharides, as well as polypeptides, proteins and nucleic acids, are presented from the point of view of their ability to form hydrogels with synthetic polymers, the preparation procedures for polymeric organic hybrid hydrogels, general physico-chemical properties and main biomedical applications (i.e., tissue engineering, wound dressing, drug delivery, etc.).

show abstract

Section: Drug and Molecule Deliverymentioning

confidence: 99%

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Vasile¹,

Pamfil²,

Stoleru³

et al. 2020

Molecules

Self Cite

210

131

View full text Add to dashboard Cite

show abstract

“…NGs are highly water-swollen, crosslinked, three-dimensional networks of hydrophilic or amphiphilic polymers in the size range of 10-1000 nm [13,14]. Due to their ability to swell in aqueous media, hydrophilic polymers such as PEG, polyvinyl alcohol (PVA), dendritic polyglycerol (dPG), chitosan, and alginate have been reported as polymeric precursors [15][16][17][18][19]. In many cases they show low and nonspecific interactions with blood proteins, making them promising candidates for tissue engineering, biomedical applications, drug delivery, and biotechnology [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Tunable Polyglycerol-Based Redox-Responsive Nanogels for Efficient Cytochrome C Delivery

et al. 2021

View full text Add to dashboard Cite

The sensitivity of therapeutic proteins is a challenge for their use in biomedical applications, as they are prone to degradation and opsonization, thus limiting their potential. This demands for the development of drug delivery systems shielding proteins and releasing them at the site of action. Here, we describe the synthesis of novel polyglycerol-based redox-responsive nanogels and report on their potential as nanocarrier systems for the delivery of cytochrome C (CC). This system is based on an encapsulation protocol of the therapeutic protein into the polymer network. NGs were formed via inverse nanoprecipitation using inverse electron-demand Diels–Alder cyclizations (iEDDA) between methyl tetrazines and norbornenes. Coprecipitation of CC led to high encapsulation efficiencies. Applying physiological reductive conditions of l-glutathione (GSH) led to degradation of the nanogel network, releasing 80% of the loaded CC within 48 h while maintaining protein functionality. Cytotoxicity measurements revealed high potency of CC-loaded NGs for various cancer cell lines with low IC50 values (up to 30 μg·mL−1), whereas free polymer was well tolerated up to a concentration of 1.50 mg·mL−1. Confocal laser scanning microscopy (CLSM) was used to monitor internalization of free and CC-loaded NGs and demonstrate the protein cargo’s release into the cytosol.

show abstract

“…The hydrophilic polymer can be modified with hydrophobic groups to form an amphiphilic polymer, which can self-assemble into nanogel in aqueous condition. Subsequently, the hydrophobic part of the nanogel can encapsulate the hydrophobic drugs by hydrophobic interaction [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Biocompatible Self-Assembled Nanogel Based on Hyaluronic Acid for Aqueous Solubility and Stability Enhancement of Asiatic Acid

et al. 2021

View full text Add to dashboard Cite

Asiatic acid (AA), a natural triterpene found in Centalla asiatica, possesses polypharmacological properties that can contribute to the treatment and prophylaxis of various diseases. However, its hydrophobic nature and rapid metabolic rate lead to poor bioavailability. The aim of this research was to develop a thermoresponsive nanogel from hyaluronic acid (HA) for solubility and stability enhancement of AA. Poly(N-isopropylacrylamide) (pNIPAM) was conjugated onto HA using a carbodiimide reaction followed by 1H NMR characterization. pNIPAM-grafted HA (HA-pNIPAM) nanogels were prepared with three concentrations of polymer, 0.1, 0.15 and 0.25% w/v, in water by the sonication method. AA was loaded into the nanogel by the incubation method. Size, morphology, AA loading capacity and encapsulation efficiency (EE) were analyzed. In vitro cytocompatibility was evaluated in fibroblast L-929 cells using the PrestoBlue assay. Single-dose toxicity was studied using rats. HA-pNIPAM nanogels at a 4.88% grafting degree showed reversible thermo-responsive behavior. All nanogel formulations could significantly increase AA water solubility and the stability was higher in nanogels prepared with high polymer concentrations over 180 days. The cell culture study showed that 12.5 µM AA in nanogel formulations was considered non-toxic to the L-929 cells; however, a dose-dependent cytotoxic effect was observed at higher AA-loaded concentrations. In vivo study proved the non-toxic effect of AA loaded in HA-pNIPAM nanogels compared with the control. Taken together, HA-pNIPAM nanogel is a promising biocompatible delivery system both in vitro and in vivo for hydrophobic AA molecules.

show abstract

Nanogels of Natural Polymers

Cited by 14 publications

References 162 publications

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Tunable Polyglycerol-Based Redox-Responsive Nanogels for Efficient Cytochrome C Delivery

In Vivo Biocompatible Self-Assembled Nanogel Based on Hyaluronic Acid for Aqueous Solubility and Stability Enhancement of Asiatic Acid

Contact Info

Product

Resources

About