Nanographene oxide‐calcium phosphate to inhibit
            <scp>
              <i>Staphylococcus aureus</i>
            </scp>
            infection and support stem cells for bone tissue engineering

Wu, Shizhou; Lei, Lei; Zhang, Hui; Liu, Jin; Weir, Michael D.; Schneider, Abraham; Zhao, Liang; Li, Jun; Xu, Hockin H.K.

doi:10.1002/term.3139

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…The addition of GO could improve the antibacterial property of bone cement and prevent the formation of bacterial biofilm, represented by Staphylococcus and E. coli. Biomaterials with CP/GO/chitosan scaffolds are expected to be excellent candidates for bone tissue engineering (Wu et al, 2020;Xie et al, 2016).…”

Section: Antimicrobial Mechanisms Of Gms In Bone Tissue Engineeringmentioning

confidence: 99%

Osteoinductive and antimicrobial mechanisms of graphene‐based materials for enhancing bone tissue engineering

Zou

Jiang

et al. 2021

J Tissue Eng Regen Med

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Graphene-based materials (GMs) have great application prospects in bone tissue engineering due to their osteoinductive ability and antimicrobial activity. GMs induce osteogenic differentiation through several mechanisms and pathways in bone tissue engineering. First of all, the surface and high hardness of the porous folds of graphene or graphene oxide (GO) can generate mechanical stimulation to initiate a cascade of reactions that promote osteogenic differentiation without any chemical inducers. In addition, change of the extracellular matrix (ECM), regulation of macrophage polarization, the oncostatin M (OSM) signaling pathway, the MAPK signaling pathway, the BMP signaling pathway, the Wnt/β-catenin signaling pathway, and other pathways are involved in GMs' regulation of osteogenesis. In bone tissue engineering, GMs prevent the formation of microbial biofilms mainly through preventing microbial adhesion and killing them. The former is mainly achieved by reducing surface free energy (SFE) and increasing hydrophobicity. The latter mainly includes oxidative stress and photothermal/photodynamic effects.Graphene and its derivatives (GDs) are mainly combined with bioactive ceramic materials, metal materials and macromolecular polymers to play an antimicrobial effect in bone tissue engineering. Concentration, number of layers, and type of GDs often affect the antimicrobial activity of GMs. In this paper, we reviewed relevant osteoinductive and antimicrobial mechanisms of GMs and their applications in bone tissue engineering.

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Section: Antimicrobial Mechanisms Of Gms In Bone Tissue Engineeringmentioning

confidence: 99%

Osteoinductive and antimicrobial mechanisms of graphene‐based materials for enhancing bone tissue engineering

Zou

Jiang

et al. 2021

J Tissue Eng Regen Med

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“…For CPC-chitosan-GO paste, the GO nano powder (XFNANO Materials Tech, Nanjing, China) was mixed with the CPC-chitosan paste to obtain a final GO concentration of 50 μg/mL. The composite disks were prepared as previously described in specific molds [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“…According to our previous study [ 9 ], the capacity of CPC-chitosan scaffold with or without GO for hDPSCs support was determined by live/dead assay for 1 day, 3 days, and 5 days culture. The percentage of live hDPSCs were examined and calculated for stem cell support.…”

Section: Methodsmentioning

confidence: 99%

“…Due to the unique properties including large surface planar structure, chemical and mechanical stability, and good biocompatibility, GO has been extensively considered as a kind of promising biomaterials for antibacterial applications 8 . Our previous in vitro study identified CPC-chitosan-GO antibacterial potential for Staphylococcus aureus [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells

Weir

Lei

et al. 2021

J Orthop Surg Res

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Background Enterococcus faecalis (E. faecalis) is the most recovered species from the root canals after failed root canal treatment. Calcium phosphate bone cement (CPC) scaffold is promising for applications in endodontic treatment as a kind of root canal sealer. Graphene oxide (GO) has been extensively considered as a kind of promising nano-materials for antibacterial applications. In the present study, an injectable CPC-chitosan paste containing GO was developed for promising endodontic therapy. The antibacterial properties of this paste against E. faecalis biofilms as well as the support for human dental pulp stem cells (hDPSCs) were investigated. Methods CPC-chitosan composite with or without GO injectable scaffold was fabricated. The hDPSC growth and viability on scaffolds were investigated by live/dead assay. Antibacterial effects against E. faecalis biofilms were determined in clinical detin block samples. Results The antibacterial CPC-chitosan-GO disks had excellent hDPSC support with the percentages of live cells at around 90%. CPC-chitosan-GO also had greater antibacterial activity on E. faecalis than that of CPC-chitosan control using detin block models (p < 0.05). Conclusions The injectable CPC-chitosan-GO paste had strong effects on inhibition E. faecalis and hDPSC support, which could fill the void of adjusting paste to the defect and shaping in situ for promising endodontic therapy.

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“…[198,199] Due to the existence of GFMs, bGBMs also present antibacterial properties. [177,[200][201][202][203][204][205][206] Moreover, the introduction of GFMs realizes the sustained release of loaded drugs and bioactive molecules. [173,182,184,[207][208][209][210] Since many articles demonstrated that the osteogenic potential of GBMs persisted even in the absence of chemical inductors, researchers tried to explore the underlying mechanotransduction.…”

Section: Osteoinductivitymentioning

confidence: 99%

Development of Graphene‐Based Materials in Bone Tissue Engineaering

et al. 2021

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Currently, general treatments of criticalsized bone defects include autografts, allografts, and the use of bone regeneration biomaterials. [1] Autograft is the gold standard approach due to its satisfying osteogenicity. But it also faces drawbacks such as an invasive surgical operation for bone harvest and scarcity of available donor sites. [2] Allograft is accompanied by the risk of disease transmission and immunological rejection. [2] Therefore, significant efforts have been devoted to finding alternative approaches for bone regeneration. Bone tissue engineering (BTE) provides a promising alternative strategy to realize higher-level restoration of bone defects. BTE exploits the elaborate combination of scaffolds, seeding cells, and biological factors to form a functional substitute or assist in situ regeneration. In most BTE research, stem cells are implanted into defective bone sites with the support of a biomaterial-based scaffold and are supposed to proliferate and differentiate into osteoblasts to promote bone regeneration. However, the performances of many current BTE systems could not meet the therapeutic expectation due to problems such as insufficient osteogenic differentiation and limited vascularization. [3][4][5] Many studies tried to improve the performance of BTE via enhancing the properties of the biomaterial-based scaffold. [6] In addition to traditional macroor microscale forms of metals, ceramics, and polymers, their nanoscale counterparts have been extensively explored for BTE due to their unique properties. [7][8][9][10] Ever since Andre Geim and Konstantin Novoselov [11] discovered a simple and feasible method for graphene isolation, graphene family materials (GFMs) have attracted great interest in regenerative medicine and tissue engineering. In BTE, bGBMs show promising potentials such as mechanical strength, high specific surface, and adsorption ability. [12][13][14][15][16][17] According to the composition, bGBMs could be categorized into GFMs and GFMs-containing composite materials (GCMs). GFMs refer to graphene and its derivatives, containing 0D graphene quantum dots (GQDs), 2D graphene (GR), graphene oxide (GO), and reduced graphene oxide (rGO), 3D graphite, etc. Among them, GQDs, GR, GO, and rGO could be obtained by chemical approaches using graphite as the starting material. GCMs are composite materials consisting of GFMs and other materials such Bone regeneration-related graphene-based materials (bGBMs) are increasingly attracting attention in tissue engineering due to their special physical and chemical properties. The purpose of this review is to quantitatively analyze mass academic literature in the field of bGBMs through scientometrics software CiteSpace, to demonstrate the rules and trends of bGBMs, thus to analyze and summarize the mechanisms behind the rules, and to provide clues for future research. First, the research status, hotspots, and frontiers of bGBMs are analyzed in an intuitively and vividly visualized way. Next, the extracted important subjects such as fabricatio...

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Nanographene oxide‐calcium phosphate to inhibit Staphylococcus aureus infection and support stem cells for bone tissue engineering

Cited by 10 publications

References 45 publications

Osteoinductive and antimicrobial mechanisms of graphene‐based materials for enhancing bone tissue engineering

Osteoinductive and antimicrobial mechanisms of graphene‐based materials for enhancing bone tissue engineering

Novel nanographene oxide-calcium phosphate cement inhibits Enterococcus faecalis biofilm and supports dental pulp stem cells

Development of Graphene‐Based Materials in Bone Tissue Engineaering

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